CDKAL1

Gene Symbol CDKAL1
Entrez Gene 54901
Alt Symbol -
Species Human
Gene Type protein-coding
Description CDK5 regulatory subunit associated protein 1-like 1
Other Description tRNA-t(6)A37 methylthiotransferase|threonylcarbamoyladenosine tRNA methylthiotransferase
Swissprots Q6P385 Q9NXB3 A8K6S0 Q5VV42 Q6ZR27
Accessions CAK32475 EAW55425 EAW55426 EAW55427 Q5VV42 AK000349 BAA91102 AK024151 AK074342 AK128546 BAC87494 AK291735 BAF84424 AK309457 AK310219 BC064145 AAH64145 BC121020 AAI21021 BC121021 AAI21022 XM_006715128 XP_006715191 XM_011514718 XP_011513020 XM_011514719 XP_011513021 XR_926265 XR_926266 XR_926267 NM_017774 NP_060244
Function Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. {ECO:0000269|PubMed:20584901}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:23048041}; Single-pass membrane protein {ECO:0000269|PubMed:23048041}. Note=Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
Tissue Specificity Expressed in pancreatic islets. {ECO:0000269|PubMed:23048041}.

CDKAL1 (D-13) - sc-135456 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
CDKAL1 (D-13): sc-135456. Western blot analysis of CDKAL1 expression in Hep G2 (A), U-2 OS (B) and Jurkat (C) whole cell lysates.

CDKAL1 (E-9) - sc-393447 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CDKAL1 (E-9): sc-393447. Western blot analysis of CDKAL1 expression in Hep G2 (A), U-2 OS (B) and Jurkat (C) whole cell lysates and human skeletal muscle (D) and human pancreas (E) tissue extracts.

CDKAL1 (H-300) - sc-368043 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CDKAL1 (H-300): sc-368043. Western blot analysis of CDKAL1 expression in Hep G2 whole cell lysate.

CDKAL1 (S-13) - sc-135459 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-CDKAL1 antibody - ab74020 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab74020 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74020 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CDKAL1 antibody - ab68045 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CDKAL1 antibody (ab68045) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-CDKAL1 antibody - ab154512 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-CDKAL1 antibody (ab154512) at 1/2000 dilution + NIH-3T3 whole cell lysate at 30 µg

Anti-CDKAL1 antibody - ab172139 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CDKAL1 antibody - ab136140 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CDKAL1 antibody (ab136140) at 1/500 dilution + HUVEC cell lysate at 30 µg

Anti-CDKAL1 antibody [EPR11621] - ab168390 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11621
All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Raji cell lysateLane 3 : MDA-MB-435 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-CDKAL1 antibody [EPR11621-26] - ab169531 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11621-26
All lanes : Anti-CDKAL1 antibody [EPR11621-26] (ab169531) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : Raji cell lysateLane 4 : MDA-MB-435 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution

Anti-CDKAL1 Antibody (Internal) - LS-C112768 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CDKAL1 Antibody (aa15-64) - LS-C291788 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CDKAL1 Antibody - LS-C341857 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CDKAL1 Antibody (N-Terminus) - LS-C345315 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of CDKAL1 Antibody with human Jurkat Whole Cell lysate.

Anti-CDKAL1 Antibody - LS-C334277 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using CDKAL1 antibody.

CDKAL1 Antibody - NBP1-32796 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CDKAL1 Antibody [NBP1-32796] - A. 30 ug Jurkat whole cell lysate/extract, B. 30 ug Raji whole cell lysate/extract, C. 30 ug K562 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.

CDKAL1 Antibody - NBP1-83660 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CDKAL1 Antibody [NBP1-83660] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissue

CDKAL1 Antibody - 44070002 from Novus Biologicals

Prices $299.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ELISA: CDKAL1 Antibody [44070002]

CDKAL1 Antibody - NBP1-36943 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

MAP 1A/1B LC3 A/B Polyclonal Antibody - bs-4309R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti MAP LC3 Beta Polyclonal Antibody, Unconjugated (bs-4309R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

CDKAL1 Polyclonal Antibody - bs-7997R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti CDKAL1 Polyclonal Antibody, Unconjugated (bs-7997R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 65kD. Observed band size: 65kD.

Anti-CDKAL1 antibody produced in rabbit - HPA014374 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CDKAL1: <B>Cat. No. HPA014374</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-CDKAL1 antibody produced in rabbit - SAB2700427 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CDKAL1: <B>Cat. No. SAB2700427</B>: Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using CDKAL1 antibody at 1:500 dilution.

CDKAL1 Antibody - N-terminal region (ARP73784_P050) - ARP73784_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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