Gene Symbol | RNF43 |
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Entrez Gene | 54894 |
Alt Symbol | RNF124, URCC |
Species | Human |
Gene Type | protein-coding |
Description | ring finger protein 43 |
Other Description | E3 ubiquitin-protein ligase RNF43 |
Swissprots | B7Z5D5 Q68DV7 B7Z443 B7Z5J5 Q65ZA4 Q6AI04 Q9NXD0 A8K4R2 |
Accessions | EAW94458 EAW94459 Q68DV7 AB081837 BAD51435 AK000322 BAA91085 AK291027 BAF83716 AK296769 BAH12429 AK298789 BAH12871 AK299024 BAH12931 AK309297 BC109028 AAI09029 CR627423 CAH10510 CR749257 CAH18113 DA457410 DA723235 XM_011524954 XP_011523256 XM_011524955 XP_011523257 XM_011524956 XP_011523258 NM_001305544 NP_001292473 NM_001305545 NP_001292474 NM_017763 NP_060233 |
Function | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. {ECO:0000269|PubMed:18313049, ECO:0000269|PubMed:22575959, ECO:0000269|PubMed:22895187}. |
Subcellular Location | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note=According to a report, may be secreted. {ECO:0000269|PubMed:15492824}. |
Tissue Specificity | Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines. {ECO:0000269|PubMed:15492824, ECO:0000269|PubMed:18313049}. |
RNF43 CRISPR/Cas9 KO Plasmid (h) - sc-403797 from Santa Cruz Biotechnology
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RNF43 HDR Plasmid (h) - sc-403797-HDR from Santa Cruz Biotechnology
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RNF43 Double Nickase Plasmid (h) - sc-403797-NIC from Santa Cruz Biotechnology
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RNF43 Double Nickase Plasmid (h2) - sc-403797-NIC-2 from Santa Cruz Biotechnology
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RNF43 CRISPR Activation Plasmid (h) - sc-403797-ACT from Santa Cruz Biotechnology
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RNF43 CRISPR Activation Plasmid (h2) - sc-403797-ACT-2 from Santa Cruz Biotechnology
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RNF43 Lentiviral Activation Particles (h) - sc-403797-LAC from Santa Cruz Biotechnology
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RNF43 Lentiviral Activation Particles (h2) - sc-403797-LAC-2 from Santa Cruz Biotechnology
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RNF43 siRNA (h) - sc-94164 from Santa Cruz Biotechnology
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RNF43 shRNA Plasmid (h) - sc-94164-SH from Santa Cruz Biotechnology
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RNF43 shRNA (h) Lentiviral Particles - sc-94164-V from Santa Cruz Biotechnology
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RNF43 RNAi - H00054894-R01 from Novus Biologicals
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MISSION® esiRNA - EHU058181 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN18438 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND18438 from Sigma-Aldrich
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RNF43 siRNA (Human) - CRJ0304 from Cohesion Biosciences
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RNF43 - MBS8237874 from MyBioSource
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shRNA set against Human RNF43 (NM_017763.4) - SHH397372 from Creative biogene
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shRNA set against Human RNF43(NM_017763.4) - SHR187464 from Creative biogene
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Human RNF43 siRNA - orb270805 from Biorbyt
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RNF43 shRNA Plasmid (Human) - CHJ0304 from Cohesion Biosciences
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RNF43 miRNA 3'UTR clone - MiUTR1H-08905 from Creative biogene
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