PAK1

Gene Symbol PAK1
Entrez Gene 5058
Alt Symbol PAKalpha
Species Human
Gene Type protein-coding
Description p21 protein (Cdc42/Rac)-activated kinase 1
Other Description STE20 homolog, yeast|alpha-PAK|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)|p65-PAK|serine/threonine-protein kinase PAK 1
Swissprots O75561 Q13153 Q13567 Q32M53 Q32M54 Q86W79
Accessions EAW75026 EAW75027 EAW75028 EAW75029 Q13153 AF071884 AAC24716 AK055228 BAG51489 AK293098 BAF85787 AK295344 BAH12038 AK296006 BAH12236 BC050377 BC109298 BC109299 AAI09300 BE501896 BE502192 BI793123 BM451179 DA393732 EU832767 ACE87778 GQ129310 ACT64444 U24152 AAA65441 U51120 AAC50590 XM_011545080 XP_011543382 XM_011545081 XP_011543383 XM_011545082 XP_011543384 XM_011545083 XP_011543385 XM_011545084 XP_011543386 NM_001128620 NP_001122092 NM_002576 NP_002567
Function Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1,
Subcellular Location Cytoplasm. Cell junction, focal adhesion. Cell membrane. Cell projection, ruffle membrane. Note=Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin.
Top Pathways Proteoglycans in cancer, Fc gamma R-mediated phagocytosis, Chemokine signaling pathway, cAMP signaling pathway, Axon guidance

Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody - 2601 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from guinea pig neutrophils stimulated with 1 µM fMLP for indicated times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (Provided by Drs. Qian Zhan and John Badwey, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts.)

Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody - 2605 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP (1 µM ) for the indicated times, using Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody.

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody - 2606 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells cotransfected with activated cdc42 and GST-PAK2 (lane 1), transfected with GST-PAK2 alone (lane 2) or activated cdc42 alone (lane 3), using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody (upper) or PAK1/2/3 Antibody #2604 (lower). This antibody detects GST-PAK2 signal only when it is cotransfected with activated cdc42, indicating that this antibody is phospho-specific.

PAK1 Antibody - 2602 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1 Antibody.

PAK1/2/3 Antibody - 2604 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1/2/3 Antibody.

αPAK (A-6) - sc-166887 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
αPAK (A-6): sc-166887. Western blot analysis of αPAK expression in SJRH30 (A) and NIH/3T3 (B) whole cell lysates.

αPAK (C-19) - sc-881 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
αPAK (C-19): sc-881. Immunofluorescence staining of methanol-fixed A-431 cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue (B) showing cytoskeletal staining.

αPAK (H-300) - sc-11394 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
αPAK (H-300): sc-11394. Western blot analysis of αPAK expression in SJRH30 whole cell lysate.

αPAK (N-20) - sc-882 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
αPAK (N-20): sc-882. Western blot analysis of αPAK expression in mouse brain (A) and rat brain (B) extracts.

α/β/γPAK (M-83) - sc-48826 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-αPAK (17.Thr 423) - sc-135755 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-αPAK (66.Thr 423) - sc-135754 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-αPAK (Thr 423) - sc-12925 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-αPAK (Ser 199/204) - sc-33531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-αPAK (Thr 212) - sc-101772 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-αPAK (Thr 212): sc-101772. Western blot analysis of phosphorylated αPAK expression in untreated (A) and forskolin-treated (B) 293 whole cell lysates.

p-α/β/γPAK (Thr 402) - sc-135684 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
p-α/β/γPAK (Thr 402): sc-135684. Western blot analysis of α/β/γPAK phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat hippocampal tissue extracts.

Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody - ab5247 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PAK1 + PAK2 + PAK3 (phospho S144 + S141 + S139) antibody - ab63513 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab63513 at a 1:50 dilution, staining PAK1 + PAK2 + PAK3 in human brain tissueLeft image un-treated.Right image treated with phosphopeptide.

Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody - ab30577 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilutionLane 1 : untreated rat hippocampal lysateLane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control)

Anti-PAK1 + PAK2 + PAK3 (phospho T423 + T402 + T421) antibody - ab62155 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab62155) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody - ab2477 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.

Anti-PAK1 + PAK2 + PAK3 antibody - ab196834 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PAK1 + PAK2 + PAK3 with ab196834 at 1/50 dilution (left) and synthesized peptide (right).

Anti-PAK1 + PAK2 + PAK3 antibody - ab131547 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labelling PAK1 + PAK2 + PAK3 with ab131547 at 1/100 dilution.

Anti-PAK1 + PAK2 + PAK3 antibody - ab59248 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59248 at 1/50 dilution staining PAK1 + PAK2 + PAK3 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunizing peptide.

Anti-PAK1 + PAK2 + PAK3 antibody - ab63259 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of PAK1, PAK2 and PAK3 expression in paraffin-embedded human brain tissue using 1/50 ab63259. Left image: untreated. Right image: sample treated with immunising peptide.
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