SLX4

Gene Symbol SLX4
Entrez Gene 84464
Alt Symbol BTBD12, FANCP, MUS312
Species Human
Gene Type protein-coding
Description SLX4 structure-specific endonuclease subunit
Other Description BTB (POZ) domain containing 12|BTB/POZ domain-containing protein 12|SLX4 structure-specific endonuclease subunit homolog|structure-specific endonuclease subunit SLX4
Swissprots Q8TF15 Q69YT8 Q96JP1 Q8IY92
Accessions EAW85345 EAW85346 EAW85347 Q8IY92 AB058687 BAB47413 AB075867 BAB85573 AK095411 AK122775 AL442083 CAH10659 BC029952 BC036335 BC104222 BC104223 BC112989 BC112990 BE887716 BM541728 XM_011522715 XP_011521017 NM_032444 NP_115820
Function Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure- specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81- EME1 endonuclease and promotes the cleavage of 3'-fl
Subcellular Location Nucleus {ECO:0000269|PubMed:19596235, ECO:0000269|PubMed:19596236}. Note=Localizes to sites of DNA dammage.
Top Pathways Fanconi anemia pathway

BTBD12 (S-12) - sc-107169 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

BTBD12 (H-39) - sc-135225 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BTBD12 (H-39): sc-135225. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear and cytoplasmic localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Anti-SLX4 antibody - ab112044 from Abcam

Prices $428.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Staining of Human SLX4 in a Formalin/PFA-fixed paraffin-embedded section of Human Prostate Carcinoma using ab112044 at a dilution of 1/250. Detection: DAB staining

Anti-SLX4 antibody - ab100997 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SLX4 antibody (ab100997) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SLX4 antibody - ab169114 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-SLX4 antibody (ab169114) at 1 µg/mlLane 1 : SLX4 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-SLX4 antibody - C-terminal - ab189591 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SLX4 antibody - C-terminal (ab189591) at 1/500 dilution (incubated for 2 hours at room temperature)Lane 1 : Mouse thymusLane 2 : Hela cell lysateLane 3 : Jurkat cell lysate

Anti-SLX4 antibody - N-terminal - ab189614 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SLX4 antibody - N-terminal (ab189614) at 1/500 dilution + Jurkat total cell extract

Anti-SLX4 Antibody (aa1650-1700) - LS-C289020 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SLX4 Antibody (aa1784-1834) - LS-C286850 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human BTBD12 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 used at a dilution of 1:250.

Anti-SLX4 Antibody (aa100-149) - LS-C135640 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SLX4 antibody LS-C135640 Western blot of ACHN lysate.

Anti-SLX4 Antibody (aa1784-1834) - LS-C289023 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Detection of Human BTBD12 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 used at a dilution of 1:1000 (1 ug/ml).

BTBD12 Antibody - NBP1-28680 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: BTBD12 Antibody [NBP1-28680] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human breast infiltrating duct carcinoma using BTBD12 antibody at 1:250 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counter-staining of the cellular nuclei with hematoxylin. This BTBD12 antibody generated a strong and very specific nuclear staining in a subset of cancerous cells in the tested section.

BTBD12 Antibody - H00084464-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: BTBD12 Antibody [H00084464-B01P] - Analysis of BTBD12 expression in transfected 293T cell line by BTBD12 polyclonal antibody. Lane 1: BTBD12 transfected lysate(126.61 KDa). Lane 2: Non-transfected lysate.

BTBD12 Antibody - NBP1-28679 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: BTBD12 Antibody [NBP1-28679] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTBD12 antibody NBP1-28679 used for IP at 10 ug/mg lysate. BTBD12 was also immunoprecipitated by rabbit anti-BTBD12 antibody NBP1-28680, which recognizes a downstream epitope. For blotting immunoprecipitated BTBD12, NBP1-28680 was used at 1 ug/ml.

BTBD12 Antibody - NBP1-71955 from Novus Biologicals

Host Rabbit
Clonality Polyclonal
Immunohistochemistry: BTBD12 Antibody [NBP1-71955]-FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 used at a dilution of 1:250

BTBD12 Antibody - NBP2-39070 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: BTBD12 Antibody [NBP2-39070] - Staining of human cervix, uterine shows moderate nucleolar positivity in squamous epithelial cells.

Anti-BTBD12 antibody produced in mouse - SAB1401894 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-BTBD12, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1401894</B>: Antibody concentration: 0.1 - 5.0 μg/mL. Specific band of ~126.61 - kDa using BTBD12 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

BTBD12 antibody - N-terminal region (ARP59857_P050) - ARP59857_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

BTBD12 antibody - 70R-9912 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

BTBD12 antibody - H00084464-B01P from Acris Antibodies

Prices $360.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot analysis of BTBD12 expression in transfected 293T cell line (H00084464-T02) by BTBD12 MaxPab polyclonal antibody. Lane 1: BTBD12 transfected lysate(126.61 KDa). Lane 2: Non-transfected lysate.

BTBD12 Antibody - GWB-MV760D from Genway Biotech

Prices $368.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SLX4 (full length) polyclonal antibody - DPABH-07724 from Creative Diagnostics

Host Mouse
Clonality Polyclonal

BTBD12 Antibody - A302-269A from Bethyl Labs

Prices $99.00, $339.00
Sizes 10 µl (1 mg/ml), 100 µl (1 mg/ml)
Host Rabbit
Clonality Polyclonal

BTBD12 Antibody - A302-270A from Bethyl Labs

Prices $99.00, $339.00
Sizes 10 µl (1 mg/ml), 100 µl (1 mg/ml)
Host Rabbit
Clonality Polyclonal

BTBD12 IHC Antibody - IHC-00643 from Bethyl Labs

Prices $360.00
Sizes 100 µl (50+ slides)
Host Rabbit
Clonality Polyclonal
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