NPC1

Gene Symbol NPC1
Entrez Gene 4864
Alt Symbol NPC
Species Human
Gene Type protein-coding
Description Niemann-Pick disease, type C1
Other Description Niemann-Pick C1 protein
Swissprots O15118 Q9P130 B4DET3
Accessions AAD48006 AAK25791 EAX01158 O15118 AB209048 BAD92285 AB971140 BAQ08368 AF002020 AAB63982 AI242474 AK293779 BAG57194 AK295715 BAG58559 AK308491 BC063302 AAH63302 BP217319 CA438246 DR159421 XM_005258277 XP_005258334 XM_005258278 XP_005258335 XM_005258279 XP_005258336 XM_006722479 XP_006722542 XM_011526015 XP_011524317 NM_000271 NP_000262
Function Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of ne
Subcellular Location Late endosome membrane {ECO:0000269|PubMed:17897319}; Multi-pass membrane protein {ECO:0000269|PubMed:17897319}. Lysosome membrane {ECO:0000269|PubMed:17897319}; Multi-pass membrane protein {ECO:0000269|PubMed:17897319}.
Top Pathways Lysosome

NPC1 (G-1) - sc-271335 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NPC1 (G-1): sc-271335. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

NPC1 (C-21) - sc-18201 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NPC1 (E-9) - sc-271334 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NPC1 (E-9): sc-271334. Western blot analysis of NPC1 expression in ES-2 whole cell lysate.

NPC1 (H-115) - sc-20152 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

NPC1 (A-20) - sc-18200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Niemann Pick C1 antibody - ab55706 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab55706 Niemann Pick C1 antibody at 1ug/ml.

Anti-Niemann Pick C1 antibody - ab108921 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Niemann Pick C1 was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to Niemann Pick C1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab108921.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 142kDa; Niemann Pick C1, non specific bands - 55kDa: We are unsure as to the identity of this extra band.

Anti-Niemann Pick C1 antibody - ab106534 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Niemann Pick C1 antibody (ab106534) at 1 µg/ml + Human kidney tissue lysate at 15 µg

Anti-Niemann Pick C1 antibody - ab116038 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Niemann Pick C1 antibody (ab116038) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

Anti-Niemann Pick C1 antibody - ab177233 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Niemann Pick C1 antibody (ab177233) at 0.5 µg/ml + HEK293 cell lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-Niemann Pick C1 antibody - ab36983 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab36983 at a 1/800 dilution staining Niemann Pick C1 in mouse 3T3-L1 cells by Immunocytochemistry/ Immunofluorescence, incubated for 12 hours at 4°C. Fixed in methanol. Blocked with 1% BSA for 1 hour 30 minutes at room temperature. Secondary used at 1/800 dilution monoclonal Goat anti-rabbit conjugated to Alexa Fluor 568 (red). Nucleus was stained with DAPI (blue).Note localization of Niemann Pick C1 around the nucleus and in cytosolic vesicles.See Abreview

Anti-Niemann Pick C1 antibody [8D10B6] - ab181458 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8D10B6
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling Niemann Pick C1 with ab181458 at 1/200 dilution, with DAB staining.

Anti-Niemann Pick C1 antibody [EPR5209] - ab134113 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5209
All lanes : Anti-Niemann Pick C1 antibody [EPR5209] (ab134113) at 1/10000 dilution (purified)Lane 1 : HepG2 cell lysateLane 2 : THP-1 cell lysateLane 3 : HEK293 cell lysateLane 4 : PC-3 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-NPC / NPC1 Antibody (aa34-174, clone 8D10B6) - LS-C285835 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8D10B6
IHC of paraffin-embedded striated muscle tissues using NPC1 mouse monoclonal antibody with DAB staining.

Anti-NPC / NPC1 Antibody IHC-plus™ - LS-B3437 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Anti-NPC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3437 concentration 10 ug/ml.

Anti-NPC / NPC1 Antibody (C-Terminus) IHC-plus™ - LS-B6977 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6977 concentration 5 ug/ml.

Anti-NPC / NPC1 Antibody (C-Terminus) - LS-C73562 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (C-Terminus, DY550) - LS-C142215 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (C-Terminus, HRP) - LS-C142214 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (C-Terminus, Biotin) - LS-C142211 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (C-Terminus, DY488) - LS-C142213 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (C-Terminus, DY650) - LS-C142212 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (aa591-620, FITC) - LS-C264604 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (aa591-620, AP) - LS-C264601 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPC / NPC1 Antibody (aa591-620, PE) - LS-C264605 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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