Gene Symbol | RNF144B |
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Entrez Gene | 255488 |
Alt Symbol | IBRDC2, PIR2, bA528A10.3, p53RFP |
Species | Human |
Gene Type | protein-coding |
Description | ring finger protein 144B |
Other Description | E3 ubiquitin-protein ligase RNF144B|IBR domain containing 2|IBR domain-containing protein 2|p53-inducible RING finger protein |
Swissprots | Q8N3R7 B3KUA8 Q7Z419 Q9BX38 Q6P4Q0 Q5TB85 B4DZI2 |
Accessions | EAW55406 EAW55407 EAW55408 EAW55409 EAW55410 Q7Z419 AB076367 BAC02434 AJ420555 AK096832 BAG53370 AK302935 BAG64094 AL832329 CAD38622 BC063311 AAH63311 EU832137 ACE87487 EU832230 ACE86800 HQ448042 ADQ32523 XM_005248984 XP_005249041 XM_005248986 XP_005249043 NM_182757 NP_877434 |
Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. {ECO:0000269|PubMed:12853982, ECO:0000269|PubMed:20300062}. |
Subcellular Location | Mitochondrion membrane {ECO:0000269|PubMed:20300062}; Single-pass membrane protein {ECO:0000269|PubMed:20300062}. Cytoplasm {ECO:0000269|PubMed:20300062}. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation. |
Tissue Specificity | Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis. {ECO:0000269|PubMed:16427630, ECO:0000269|PubMed:20300062}. |
p53RFP CRISPR/Cas9 KO Plasmid (h) - sc-406081 from Santa Cruz Biotechnology
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p53RFP HDR Plasmid (h) - sc-406081-HDR from Santa Cruz Biotechnology
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p53RFP Double Nickase Plasmid (h) - sc-406081-NIC from Santa Cruz Biotechnology
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p53RFP Double Nickase Plasmid (h2) - sc-406081-NIC-2 from Santa Cruz Biotechnology
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p53RFP CRISPR Activation Plasmid (h) - sc-406081-ACT from Santa Cruz Biotechnology
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p53RFP CRISPR Activation Plasmid (h2) - sc-406081-ACT-2 from Santa Cruz Biotechnology
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p53RFP Lentiviral Activation Particles (h) - sc-406081-LAC from Santa Cruz Biotechnology
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p53RFP Lentiviral Activation Particles (h2) - sc-406081-LAC-2 from Santa Cruz Biotechnology
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p53RFP siRNA (h) - sc-44824 from Santa Cruz Biotechnology
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p53RFP shRNA Plasmid (h) - sc-44824-SH from Santa Cruz Biotechnology
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p53RFP shRNA (h) Lentiviral Particles - sc-44824-V from Santa Cruz Biotechnology
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IBRDC2 RNAi - H00255488-R01 from Novus Biologicals
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MISSION® esiRNA - EHU077511 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN18388 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND18388 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR14091 from Sigma-Aldrich
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RNF144B siRNA (Human) - CRJ6723 from Cohesion Biosciences
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RNF144B - MBS829015 from MyBioSource
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shRNA set against Human RNF144B (NM_182757.3) - SHH396784 from Creative biogene
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RNF144B shRNA Plasmid (Human) - CHJ6723 from Cohesion Biosciences
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RNF144B miRNA 3'UTR clone - MiUTR1H-04772 from Creative biogene
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