E2F7

Gene Symbol E2F7
Entrez Gene 144455
Alt Symbol -
Species Human
Gene Type protein-coding
Description E2F transcription factor 7
Other Description E2F-7|transcription factor E2F7
Swissprots Q96AV8 B5MED9 B2RMR7 B3KUP8 A6NC74 B3KTZ5
Accessions EAW97338 EAW97339 EAW97340 Q96AV8 AI380315 AK023043 AK096316 BAG53257 AK097677 BAG53510 BC016658 AAH16658 BC017481 BC136366 AAI36367 BC136367 AAI36368 DB159890 XM_011537966 XP_011536268 XM_011537967 XP_011536269 XM_011537968 XP_011536270 XM_011537969 XP_011536271 NM_203394 NP_976328
Function Atypical E2F transcription factor that participates in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'- TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1. Acts as a regulator of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-3' and mediating repression of G1/S-regulated genes. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Also involved in DNA damage response: up-regulated by p53/TP53 following genotoxic stress and acts as a downstream effector of p53/TP53-dep
Subcellular Location Nucleus {ECO:0000269|PubMed:15133492}.

E2F-7 (H-300) - sc-66870 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

E2F-7 (N-20) - sc-32574 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
E2F-7 (N-20): sc-32574. Western blot analysis of E2F-7 expression in NTERA-2 cl.D1 (A), HeLa (B) and Jurkat (C) whole cell lysates.

E2F-7 (R-17) - sc-32575 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-E2F7 antibody - ab56022 from Abcam

Prices $379.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-E2F7 antibody (ab56022) at 0.5 µg/ml + SP2/0 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-E2F7 antibody - ab106832 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-E2F7 antibody (ab106832) at 0.1 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-E2F7 antibody - ab123864 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-E2F7 antibody (ab123864) at 1/100 dilution + A2058 cell line lysate at 35 µg

Anti-E2F7 antibody - N-terminal - ab166727 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa591-619, AP) - LS-C234828 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa591-619, APC) - LS-C234829 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa591-619, Biotin) - LS-C234830 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa591-619, FITC) - LS-C234831 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa53-66) - LS-C112753 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
E2F7 antibody (0.1 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-E2F7 Antibody (aa1-50) - LS-C289739 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa591-619, PE) - LS-C234834 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa591-619, Azide-free, HRP) - LS-C249883 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa861-911) - LS-C289742 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa590-619) - LS-C320995 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-E2F7 Antibody (aa591-619) - LS-C167600 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
E2F7 Antibody western blot of A2058 cell line lysates (35 ug/lane). The E2F7 antibody detected the E2F7 protein (arrow).

Anti-E2F7 Antibody (aa108-157) - LS-C31453 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
SP2/0 Cell Lysate

Anti-E2F7 Antibody - LS-C348746 from LifeSpan Bioscience

Prices $590.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
The antibody recognizes E2F7 isoform 2 (E2F7-A), but not E2F7 isoform 1 (E2F7-B). Whole cell extracts (10 ug) from Saos-2 cells transfected with either mock pcDNA3 (None), pcDNA3-HA-E2F7-A (E2F7-A), or pcDNA3-HAE2F7- B (E2F7-B) were fractionated on an 8% SDS-PAGE, transferred to nitrocellulose membranes, and probed with a previously described anti-E2F7 antibody (Control) (1) or the anti-E2F7-A antibody. Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. As shown in panel B, the antibody recognizes E2F7-A, but not E2F7-B.

Anti-E2F7 Antibody - LS-C348747 from LifeSpan Bioscience

Prices $590.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
The antibody recognizes E2F7 isoform 1 (E2F7-B), but not E2F7 isoform 2 (E2F7-A). Whole cell extracts (10 ug) from Saos-2 cells transfected with either mock pcDNA3 (None), pcDNA3-HA-E2F7-A (E2F7-A), or pcDNA3-HAE2F7-B (E2F7-B) were fractionated on an 8% SDS-PAGE, transferred to nitrocellulose membranes, and probed with a previously described anti-E2F7 antibody (Control) (1) or the anti-E2F7antibody. Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. As shown in panel B, the antibody recognizes E2F7-B, but not E2F7-A.

Anti-E2F7 Antibody - LS-C348749 from LifeSpan Bioscience

Prices $590.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
The anti-E2F7 antibody is able to supershift DNA-protein complexes that contain either E2F7-A or E2F7-B. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from Saos-2 cells transfected with either pcDNA3-HA-E2F7-A (A) or pcDNA3-HA-E2F7-B (B) were analyzed by EMSA. Extracts were preincubated in the presence of an antibody to the HA tag, the anti-E2F7-B antibody, the anti-E2F7 antibody, or the corresponding pre-immune serum (PI). The arrow indicates the position of the HA-E2F7-A- or HA-E2F7-B-containing complexes, whereas arrowheads indicate the position of super-shifted complexes.

E2F7 Antibody - NBP1-52649 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: E2F7 Antibody [NBP1-52649] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.   Affinity purified rabbit anti-E2F7 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  E2F7 was also immunoprecipitated by rabbit anti-E2F7 antibody NBP1-52648, which recognizes an upstream epitopes.

E2F7 Antibody - NBP1-52648 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: E2F7 Antibody [NBP1-52648] -  Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-E2F7 antibody NBP1-52648 used for IP at 6 ug/mg lysate. E2F7 was also immunoprecipitated by rabbit anti-E2F7 antibody NBP1-52649, which recognizes a downstream epitope. For blotting immunoprecipitated E2F7, NBP1-52649 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

E2F7 Antibody - NBP1-26399 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: E2F7 Antibody [NBP1-26399] - (0.3ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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