DROSHA
| Gene Symbol | DROSHA |
|---|---|
| Entrez Gene | 29102 |
| Alt Symbol | ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN |
| Species | Human |
| Gene Type | protein-coding |
| Description | drosha, ribonuclease type III |
| Other Description | RNase III|drosha, double-stranded RNA-specific endoribonuclease|nuclear RNase III Drosha|p241|protein Drosha|putative protein p241 which interacts with transcription factor Sp1|putative ribonuclease III|ribonuclease 3|ribonuclease III, nuclear|ribonuclease type III, nuclear |
| Swissprots | Q9Y4Y0 Q7Z5V2 Q86YH0 Q9Y2V9 E7EMP9 Q9NRR4 Q9NW73 |
| Accessions | CBM42910 CBV35643 EAX10767 EAX10768 EAX10769 EAX10770 Q9NRR4 AA917625 AB209501 BAD92738 AF116910 AAD29637 AF189011 AAF80558 AJ242976 CAB45133 AK001121 BAA91511 AK026563 AK308093 BC024261 BC041162 AAH41162 BC054003 AAH54003 BX647724 DB065470 XM_005248291 XP_005248348 XM_005248292 XP_005248349 XM_005248293 XP_005248350 XM_005248294 XP_005248351 XM_011514033 XP_011512335 NM_001100412 NP_001093882 NM_013235 NP_037367 |
| Function | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. {ECO:0000269|PubMed:10948199, ECO:0000269|PubMed:14508493, ECO:0000269|PubMed:15531877, ECO:0000269|PubMed:15565168, ECO:0000269|PubMed:15574589, ECO:0000269|PubMed:15589161, ECO:0000269|PubMed:16751099, ECO:0000269|PubMed:16906129, ECO:0000269|PubMed:17159994}. |
| Subcellular Location | Nucleus. Nucleus, nucleolus. Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. |
| Tissue Specificity | Ubiquitous. {ECO:0000269|PubMed:10948199}. |
| Top Pathways | Ribosome biogenesis in eukaryotes, Proteoglycans in cancer |
RNase III Drosha CRISPR/Cas9 KO Plasmid (h) - sc-401639 from Santa Cruz Biotechnology
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RNase III Drosha HDR Plasmid (h) - sc-401639-HDR from Santa Cruz Biotechnology
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RNase III Drosha Double Nickase Plasmid (h) - sc-401639-NIC from Santa Cruz Biotechnology
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RNase III Drosha Double Nickase Plasmid (h2) - sc-401639-NIC-2 from Santa Cruz Biotechnology
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RNase III Drosha CRISPR Activation Plasmid (h) - sc-401639-ACT from Santa Cruz Biotechnology
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RNase III Drosha CRISPR Activation Plasmid (h2) - sc-401639-ACT-2 from Santa Cruz Biotechnology
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RNase III Drosha Lentiviral Activation Particles (h) - sc-401639-LAC from Santa Cruz Biotechnology
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RNase III Drosha Lentiviral Activation Particles (h2) - sc-401639-LAC-2 from Santa Cruz Biotechnology
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RNase III Drosha siRNA (h) - sc-44080 from Santa Cruz Biotechnology
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RNase III Drosha shRNA Plasmid (h) - sc-44080-SH from Santa Cruz Biotechnology
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RNase III Drosha shRNA (h) Lentiviral Particles - sc-44080-V from Santa Cruz Biotechnology
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Drosha RNAi - H00029102-R01 from Novus Biologicals
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MISSION® esiRNA - EHU070741 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7493 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7493 from Sigma-Aldrich
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DROSHA siRNA (Human) - CRH9193 from Cohesion Biosciences
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DROSHA - MBS8213023 from MyBioSource
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shRNA set against Human DROSHA (NM_013235.4) - SHH281097 from Creative biogene
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shRNA set against Human RNASEN(NM_013235.4) - SHH220614 from Creative biogene
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DROSHA shRNA Plasmid (Human) - CHH9193 from Cohesion Biosciences
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DROSHA miRNA 3'UTR clone - MiUTR1H-08864 from Creative biogene
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DROSHA miRNA 3'UTR clone - MiUTR3H-10252 from Creative biogene
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