SF3B6

Gene Symbol SF3B6
Entrez Gene 51639
Alt Symbol CGI-110, HSPC175, Ht006, P14, SAP14, SAP14a, SF3B14, SF3B14a
Species Human
Gene Type protein-coding
Description splicing factor 3b, subunit 6, 14kDa
Other Description SF3b 14 kDa subunit|pre mRNA branch site protein p14|pre-mRNA branch site protein p14|spliceosome-associated protein, 14 kDa subunit|spliceosome-associated protein, 14-kDa|splicing factor 3B subunit 6|splicing factor 3B, 14 kDa subunit
Swissprots Q9Y3B4 Q53TM1
Accessions AAY14664 EAX00767 Q9Y3B4 AF151868 AAD34105 AF161523 AAF29138 AF184213 AAG09698 AF401310 AAK94041 AK000621 AK311118 AK311908 BAG34849 BC015463 AAH15463 BG200650 JF432474 ADZ15691 NM_016047 NP_057131
Function Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively. {ECO:0000269|PubMed:16432215}.
Subcellular Location Nucleus {ECO:0000305}.
Top Pathways Spliceosome

SAP 14 (A-12) - sc-161212 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SAP 14 (Q-14) - sc-161213 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SAP 14 (T-13) - sc-161214 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-SF3B14 antibody - ab74676 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab74676 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74676 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SF3B6 / SF3B14 Antibody (C-Terminus) - LS-C80376 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Placenta cell lysate. Antibody concentration: 2.0 ug/ml. Gel concentration: 6%-18%.

Anti-SF3B6 / SF3B14 Antibody (N-Terminus) - LS-C31803 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
SF3B1 antibody LS-C31803 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.

Anti-SF3B6 / SF3B14 Antibody (aa131-180) - LS-C31739 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
B: Jurkat Cell Lysate

Anti-SF3B6 Antibody (aa200-250) - LS-C75689 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-SF3B6 Antibody (aa100-150) - LS-C38994 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-SF3B6 / SF3B14 Antibody (aa90-139) - LS-C31740 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
SF3B1 antibody LS-C31740 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.

Anti-SF3B6 / SF3B14 Antibody (aa76-125) - LS-C120241 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HepG2 cells, using SF3B14 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-SF3B6 / SF3B14 Antibody (N-Terminus) - LS-C110571 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SF3B14 antibody LS-C110571 Western blot of THP-1 cell lysate.

SF3B14 Antibody - NBP1-87431 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SF3B14 Antibody [NBP1-87431] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

SF3B14 Antibody - NBP1-87430 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SF3B14 Antibody - NBP1-57226 from Novus Biologicals

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: SF3B14 Antibody [NBP1-57226] - THP-1 cell lysate, concentration 0.2-1 ug/ml.

Anti-SF3B14 antibody produced in rabbit - AV40829 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: placenta (<B>Cat. No. AV40829</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 2 μg/mL in cell type placenta

Anti-SF3B14 antibody produced in rabbit - HPA034829 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-AC008073.5: <B>Cat. No. HPA034829</B>: Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Anti-SF3B14 antibody produced in rabbit - HPA034830 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-AC008073.5: <B>Cat. No. HPA034830</B>: Immunohistochemical staining of human placenta shows strong nuclear positivity in decidual cells.

Anti-SF3B14, (N-terminal) antibody produced in rabbit - SAB2103789 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SF3B14, antibody produced in rabbit: <B>Cat. No. SAB2103789</B>: Immunoblotting analysis of SF3B14 in THP-1 cell lysate with SF3B14 anitbody at 1 μg/mL.

Anti-SF3B14, C-Terminal antibody produced in rabbit - SAB4502154 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SF3B14, C-Terminal: <B>Cat. No. SAB4502154</B>: Immunoblotting analysis of extracts from HepG2 cells, using Anti-SF3B14, C-Terminal antibody. The lane on the left was treated with the Anti-SF3B14, C-Terminal antibody. The lane on the right (negative control) was treated with both Anti-SF3B14, C-Terminal antibody and the synthesized immunogen peptide.

SF3B14 antibody - N-terminal region (ARP40828_P050) - ARP40828_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SF3B14 antibody - middle region (ARP40829_P050) - ARP40829_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SAP14-Antibody - AP51508 from Abgent, a WuXi AppTec company

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SAP14 Antibodyat 1:1000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

SF3B14 Antibody (OAAF04050) - OAAF04050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

SF3B14 antibody - 70R-4706 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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