CHFR
| Gene Symbol | CHFR |
|---|---|
| Entrez Gene | 55743 |
| Alt Symbol | RNF116, RNF196 |
| Species | Human |
| Gene Type | protein-coding |
| Description | checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase |
| Other Description | E3 ubiquitin-protein ligase CHFR|RING finger protein 196 |
| Swissprots | Q9NVD5 Q9NRT4 B4DZ77 B4E2L6 Q9NT32 Q96SL3 Q96EP1 A6NEN5 |
| Accessions | EAW54809 EAW54810 EAW54811 EAW54812 EAW54813 EAW54814 EAW54815 Q96EP1 AF170724 AAF91084 AK001658 BAA91817 AK027687 BAB55297 AK054917 AK090948 AK097671 BAG53509 AK302785 BAG63989 AK304333 BAG65178 AL137561 CAB70812 BC012072 AAH12072 BP313700 DQ599878 DQ892232 ABM83158 DQ895433 ABM86359 NM_001161344 NP_001154816 NM_001161345 NP_001154817 NM_001161346 NP_001154818 NM_001161347 NP_001154819 NM_018223 NP_060693 |
| Function | E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. { |
| Subcellular Location | Nucleus, PML body {ECO:0000269|PubMed:11912157, ECO:0000269|PubMed:15467728, ECO:0000269|PubMed:18172500}. |
| Tissue Specificity | Ubiquitous. {ECO:0000269|PubMed:10935642}. |
Chfr CRISPR/Cas9 KO Plasmid (h) - sc-408259 from Santa Cruz Biotechnology
|
||||||||
Chfr HDR Plasmid (h) - sc-408259-HDR from Santa Cruz Biotechnology
|
||||||||
Chfr Double Nickase Plasmid (h) - sc-408259-NIC from Santa Cruz Biotechnology
|
||||||||
Chfr Double Nickase Plasmid (h2) - sc-408259-NIC-2 from Santa Cruz Biotechnology
|
||||||||
Chfr CRISPR Activation Plasmid (h) - sc-408259-ACT from Santa Cruz Biotechnology
|
||||||||
Chfr CRISPR Activation Plasmid (h2) - sc-408259-ACT-2 from Santa Cruz Biotechnology
|
||||||||
Chfr Lentiviral Activation Particles (h) - sc-408259-LAC from Santa Cruz Biotechnology
|
||||||||
Chfr Lentiviral Activation Particles (h2) - sc-408259-LAC-2 from Santa Cruz Biotechnology
|
||||||||
Chfr siRNA (h) - sc-37567 from Santa Cruz Biotechnology
|
||||||||
Chfr shRNA Plasmid (h) - sc-37567-SH from Santa Cruz Biotechnology
|
||||||||
Chfr shRNA (h) Lentiviral Particles - sc-37567-V from Santa Cruz Biotechnology
|
||||||||
CHFR RNAi - H00055743-R01V from Novus Biologicals
|
||||||||
CHFR RNAi - H00055743-R01 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU090571 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN6052 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6052 from Sigma-Aldrich
|
||||||||
CHFR siRNA (Human) - CRJ0881 from Cohesion Biosciences
|
||||||||
CHFR - MBS8236289 from MyBioSource
|
||||||||
shRNA set against Human CHFR (NM_018223.2) - SHH262985 from Creative biogene
|
||||||||
Human CHFR siRNA - orb271382 from Biorbyt
|
||||||||
CHFR shRNA Plasmid (Human) - CHJ0881 from Cohesion Biosciences
|
||||||||
CHFR miRNA 3'UTR clone - MiUTR3H-07453 from Creative biogene
|
||||||||
CHFR miRNA 3'UTR clone - MiUTR3H-07454 from Creative biogene
|
||||||||
CHFR miRNA 3'UTR clone - MiUTR3H-07455 from Creative biogene
|
||||||||
CHFR miRNA 3'UTR clone - MiUTR3H-07456 from Creative biogene
|