TRIM33

Gene Symbol TRIM33
Entrez Gene 51592
Alt Symbol ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA
Species Human
Gene Type protein-coding
Description tripartite motif containing 33
Other Description E3 ubiquitin-protein ligase TRIM33|RET-fused gene 7 protein|TIF1-gamma|ectodermin homolog|protein Rfg7|transcriptional intermediary factor 1 gamma|tripartite motif-containing 33
Swissprots Q9C017 Q5TG74 Q9UPN9 Q9UJ79 Q5TG72 O95855 Q5TG73
Accessions EAW56599 EAW56600 EAW56601 Q9UPN9 AB029036 BAA83065 AB075517 AF119043 AAD17259 AF220136 AAG53509 AF220137 AAG53510 AJ132948 CAB55313 AK021491 BAB13834 AK023434 BAG51192 AK057487 AK301997 BAG63401 AL080216 CN267696 XM_005270936 XP_005270993 XM_005270937 XP_005270994 XM_011541568 XP_011539870 NM_015906 NP_056990 NM_033020 NP_148980
Function Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade). {ECO:0000250, ECO:0000269|PubMed:10022127, ECO:0000269|PubMed:15820681, ECO:0000269|PubMed:16751102, ECO:0000269|PubMed:19135894}.
Subcellular Location Nucleus {ECO:0000269|PubMed:15820681, ECO:0000269|PubMed:16751102, ECO:0000269|PubMed:19135894}. Note=In discrete nuclear dots resembling nuclear bodies. {ECO:0000250}.
Tissue Specificity Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

TRIM33 (E1N2Z) Rabbit mAb - 13387 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1N2Z
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® TRIM33 siRNA I #13487 (+), or SignalSilence ® siRNA II #13503 (+), using TRIM33 (E1N2Z) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). The TRIM33 (E1N2Z) Rabbit mAb confirms silencing of TRIM33, while the GAPDH (D16H11) XP ® Rabbit mAb is used as a loading control.

TRIM33 Antibody - 8972 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

TIF1γ (C-19) - sc-69040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TIF1γ (H-106) - sc-68424 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TIF1γ (H-106): sc-68424. Western blot analysis of TIF1γ expression in SW480 whole cell lysate.

TIF1γ (XX-19) - sc-101179 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
TIF1γ (XX-19): sc-101179. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing nuclear localization.

Anti-TIF1 gamma antibody - ab72193 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling TIF1 gamma with ab72193 at 1/200 (1µg/ml). Detection: DAB.

Anti-TIF1 gamma antibody - ab47062 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab47062 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47062, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

Anti-TIF1 gamma antibody - ab84455 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate adenocarcinoma using 1/250 ab84455, followed by DAB staining.

Anti-TIF1 gamma antibody - ab57172 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
TIF1 gamma antibody (ab57172) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.

Anti-TIF1 gamma antibody - ab86314 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-TIF1 gamma antibody (ab86314) at 1 µg/ml + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-TIF1 gamma antibody [16G9] - ab33475 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 16G9
Anti-TIF1 gamma antibody [16G9] (ab33475) at 2 µg/ml + Recombinant TIF1 gamma at 20 µg

Anti-TIF1 gamma antibody [3H7-C8-B9] - ab151266 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3H7-C8-B9
All lanes : Anti-TIF1 gamma antibody [3H7-C8-B9] (ab151266) at 1/1000 dilutionLane 1 : MCF7 nuclear extractLane 2 : HeLa nuclear extract

Anti-TIF1 gamma antibody - C-terminal - ab170789 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-TIF1 gamma antibody - C-terminal (ab170789) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

Anti-TRIM33 / TIF1-Gamma Antibody (aa1-50) - LS-C89720 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (C-Terminus) - LS-C89722 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (Internal) - LS-C209946 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (Internal) - LS-C210006 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (C-Terminus, AP) - LS-C218122 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (C-Terminus, APC) - LS-C218123 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (C-Terminus, Biotin) - LS-C218124 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (aa1095-1125) - LS-C160188 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
TRIM33 Antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The TRIM33 antibody detected the TRIM33 protein (arrow).

Anti-TRIM33 / TIF1-Gamma Antibody (C-Terminus, FITC) - LS-C218125 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (C-Terminus, Azide-free, HRP) - LS-C218126 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM33 / TIF1-Gamma Antibody (aa820-849) - LS-C160189 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
TRIM33 Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The TRIM33 antibody detected the TRIM33 protein (arrow).

Anti-TRIM33 / TIF1-Gamma Antibody (C-Terminus, PE) - LS-C218128 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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