RBX1

Gene Symbol RBX1
Entrez Gene 9978
Alt Symbol BA554C12.1, RNF75, ROC1
Species Human
Gene Type protein-coding
Description ring-box 1, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase RBX1|RING box protein 1|RING finger protein 75|RING-box protein 1|ZYP protein|regulator of cullins 1
Swissprots Q9D1S2 B2RDY1 Q9Y254 Q8N6Z8 Q9WUK9 P62877
Accessions CAT16469 CDM22122 EAW60402 EAW60403 P62877 AF085906 AF140598 AAD29715 AF142059 AAD30146 AK090764 AK315722 BAG38078 AY099360 AAM21718 BC001466 AAH01466 BC017370 AAH17370 CR456560 CAG30446 CU013160 CAK54591 CU013448 CAK54890 DA353856 DN602016 NM_014248 NP_055063
Function E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11027288}. Nucleus {ECO:0000269|PubMed:11027288}.
Tissue Specificity Widely expressed.
Top Pathways Wnt signaling pathway, Nucleotide excision repair, Renal cell carcinoma, Circadian rhythm, Oocyte meiosis

RBX1 (D3J5I) Rabbit mAb - 11922 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3J5I
Western blot analysis of extracts from various cell lines using RBX1 (D3J5I) Rabbit mAb.

RBX1 Antibody - 4397 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, Hep G2, and HCT 116 cells using RBX1 Antibody.

Rbx1 (C-15) - sc-5200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rbx1 (C-15): sc-5200. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells, cells in granular layer and cells in molecular layer.

Rbx1 (H-65) - sc-366959 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rbx1 (H-65): sc-366959. Western blot analysis of Rbx1 expression in HeLa (A), Hep G2 (B), Jurkat (C), MCF7 (D), HT-1080 (E) and HCT-116 (F) whole cell lysates.

Rbx1 (I-16) - sc-5202 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Rbx1 (N-16) - sc-5201 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-ROC1 antibody - ab86862 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ROC1 antibody (ab86862) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ROC1 antibody - ab2977 from Abcam

Prices $390.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab2977 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2977, 5&microg/ml) overnight at +4&degC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43&microM.

Anti-ROC1 antibody - ab116253 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab116253, at a 1/500 dilution, staining ROC1 in Formalin-fixed, Paraffin-embedded Human brain (cerebellum) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ROC1 antibody - ab140251 from Abcam

Prices $370.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-ROC1 antibody - ab169035 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-ROC1 antibody [EPR6849(B)] - ab124950 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6849(B)
All lanes : Anti-ROC1 antibody [EPR6849(B)] (ab124950) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.Secondarystandard HRP labelled goat anti-rabbit at 1/2000 dilution

Anti-ROC1 antibody [EPR6850(B)] - ab133565 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6850(B)
Overlay histogram showing HeLa cells stained with ab133565 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133565, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-ROC1 antibody, prediluted - ab15517 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15517 staining ROC1 in human tonsil by Immunohistochemistry (FFPE-sections).

Anti-RBX1 / ROC1 Antibody (C-Terminus) - LS-C30557 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody (C-Terminus) - LS-C38940 from LifeSpan Bioscience

Prices $455.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody (C-Terminus) - LS-C131934 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody (aa94-108, Azide-free) - LS-C88599 from LifeSpan Bioscience

Prices $465.00, $740.00
Sizes 500 µg, 1 mg
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody (N-Terminus) - LS-C131935 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody (aa94-108) - LS-C88598 from LifeSpan Bioscience

Prices $255.00, $435.00, $680.00
Sizes 100 µg, 500 µl, 1 mg
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody (C-Terminus) - LS-C189188 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody - LS-C341353 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RBX1 / ROC1 Antibody (N-Terminus, clone EPR6849(B)) - LS-C138574 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6849(B)
Western blot analysis on (A) HepG2, (B) Jurkat, and (C) HeLa cell lysates using anti-RBX1 antibody.

Anti-RBX1 / ROC1 Antibody (C-Terminus) IHC-plus™ - LS-B5298 from LifeSpan Bioscience

Prices $395.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
Anti-RBX1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5298 dilution 1:50.

Anti-RBX1 / ROC1 Antibody - LS-C335186 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using RBX1 antibody.
Search more