RNF128
| Gene Symbol | RNF128 |
|---|---|
| Entrez Gene | 79589 |
| Alt Symbol | GRAIL |
| Species | Human |
| Gene Type | protein-coding |
| Description | ring finger protein 128, E3 ubiquitin protein ligase |
| Other Description | E3 ubiquitin-protein ligase RNF128|gene related to anergy in lymphocytes protein |
| Swissprots | A0PJI4 Q6PH80 Q9H5E4 Q6ZTJ8 Q8TEB7 Q96RF3 |
| Accessions | EAX02737 Q8TEB7 AF394689 AAK77554 AK027169 BAB15682 AK074264 BAB85033 AK126553 BAC86589 BC012931 BC030951 AAH30951 BC036901 BC056677 AAH56677 BC063404 AAH63404 BI600046 HQ448134 ADQ32615 NM_024539 NP_078815 NM_194463 NP_919445 |
| Function | E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys- 48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals. {ECO:0000269|PubMed:12705856, ECO:0000269|PubMed:22016387}. |
| Subcellular Location | Endomembrane system {ECO:0000250}; Single- pass membrane protein {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:12705856, ECO:0000269|PubMed:22016387}. Cytoplasm, perinuclear region {ECO:0000250}. Note=Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity). {ECO:0000250}. |
GRAIL CRISPR/Cas9 KO Plasmid (h) - sc-407089 from Santa Cruz Biotechnology
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GRAIL HDR Plasmid (h) - sc-407089-HDR from Santa Cruz Biotechnology
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GRAIL Double Nickase Plasmid (h) - sc-407089-NIC from Santa Cruz Biotechnology
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GRAIL Double Nickase Plasmid (h2) - sc-407089-NIC-2 from Santa Cruz Biotechnology
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GRAIL CRISPR Activation Plasmid (h) - sc-407089-ACT from Santa Cruz Biotechnology
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GRAIL CRISPR Activation Plasmid (h2) - sc-407089-ACT-2 from Santa Cruz Biotechnology
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GRAIL Lentiviral Activation Particles (h) - sc-407089-LAC from Santa Cruz Biotechnology
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GRAIL Lentiviral Activation Particles (h2) - sc-407089-LAC-2 from Santa Cruz Biotechnology
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GRAIL siRNA (h) - sc-90884 from Santa Cruz Biotechnology
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GRAIL shRNA Plasmid (h) - sc-90884-SH from Santa Cruz Biotechnology
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GRAIL shRNA (h) Lentiviral Particles - sc-90884-V from Santa Cruz Biotechnology
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GRAIL/RNF128 RNAi - H00079589-R02 from Novus Biologicals
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MISSION® esiRNA - EHU084411 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN18378 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND18378 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08981 from Sigma-Aldrich
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RNF128 siRNA (Human) - CRJ2411 from Cohesion Biosciences
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GRAIL/RNF128 RNAi - H00079589-R01 from Novus Biologicals
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RNF128 - MBS8216440 from MyBioSource
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shRNA set against Human Rnf12 (NM_194463.1) - SHH396600 from Creative biogene
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shRNA set against Human RNF128(NM_194463.1) - SHR185032 from Creative biogene
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shRNA set against Human RNF128(NM_024539.3) - SHR185034 from Creative biogene
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shRNA set against Human RNF128(NM_194463.1) - SHR185376 from Creative biogene
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shRNA set against Human RNF128(NM_024539.3) - SHR185392 from Creative biogene
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RNF128 shRNA Plasmid (Human) - CHJ2411 from Cohesion Biosciences
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