POLK
| Gene Symbol | POLK |
|---|---|
| Entrez Gene | 51426 |
| Alt Symbol | DINB1, DINP, POLQ |
| Species | Human |
| Gene Type | protein-coding |
| Description | polymerase (DNA directed) kappa |
| Other Description | DNA polymerase kappa |
| Swissprots | Q96Q87 Q96L01 Q8IZY0 B2RBD2 Q96Q86 Q5Q9G6 Q8NB30 Q5Q9G5 Q86VJ8 Q9UBT6 Q8IZY1 Q5Q9G7 Q9UHC5 Q5Q9G8 |
| Accessions | AAP12648 BAB58975 BAB58976 CCQ43535 EAW95762 EAW95763 EAW95764 EAW95765 Q9UBT6 AB027564 BAA86943 AB209291 BAD92528 AF163570 AAF02540 AF194973 AAF23270 AF315601 AF315602 AAN15780 AF318313 AAN15781 AK026316 AK091659 BAC03714 AK314610 BAG37179 AY769929 AAV80827 AY769930 AAV80828 AY769931 AAV80829 AY769932 AAV80830 BC014955 AAH14955 BC041798 BC050718 AAH50718 XM_005248534 XP_005248591 XM_005248536 XP_005248593 XM_006714652 XP_006714715 XM_011543463 XP_011541765 XM_011543464 XP_011541766 XM_011543465 XP_011541767 XM_011543466 XP_011541768 XM_011543467 XP_011541769 XM_011543468 XP_011541770 XR_241783 XR_241784 XR_948273 NM_016218 NP_057302 |
| Function | DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. {ECO:0000269|PubMed:10620008, ECO:0000269|PubMed:11024016, ECO:0000269|PubMed:12145297, ECO:0000269|PubMed:12444249, ECO:0000269|PubMed:12952891, ECO:0000269|PubMed:14630940, ECO:0000269|PubMed:15533436}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:12414988}. Note=Detected throughout the nucleus and at replication foci. |
| Tissue Specificity | Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes. {ECO:0000269|PubMed:10518552, ECO:0000269|PubMed:10620008}. |
| Top Pathways | Fanconi anemia pathway |
DinB CRISPR/Cas9 KO Plasmid (h) - sc-405052 from Santa Cruz Biotechnology
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DinB HDR Plasmid (h) - sc-405052-HDR from Santa Cruz Biotechnology
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DinB Double Nickase Plasmid (h) - sc-405052-NIC from Santa Cruz Biotechnology
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DinB Double Nickase Plasmid (h2) - sc-405052-NIC-2 from Santa Cruz Biotechnology
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DinB CRISPR Activation Plasmid (h) - sc-405052-ACT from Santa Cruz Biotechnology
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DinB CRISPR Activation Plasmid (h2) - sc-405052-ACT-2 from Santa Cruz Biotechnology
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DinB Lentiviral Activation Particles (h) - sc-405052-LAC from Santa Cruz Biotechnology
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DinB Lentiviral Activation Particles (h2) - sc-405052-LAC-2 from Santa Cruz Biotechnology
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DinB siRNA (h) - sc-60537 from Santa Cruz Biotechnology
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DinB shRNA Plasmid (h) - sc-60537-SH from Santa Cruz Biotechnology
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DinB shRNA (h) Lentiviral Particles - sc-60537-V from Santa Cruz Biotechnology
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DNA polymerase theta RNAi - H00010721-R02 from Novus Biologicals
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MISSION® esiRNA - EHU082021 from Sigma-Aldrich
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MISSION® siRNA - SIHK1769 from Sigma-Aldrich
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MISSION® siRNA - SIHK1767 from Sigma-Aldrich
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MISSION® siRNA - SIHK1768 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17101 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17101 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08765 from Sigma-Aldrich
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POLK siRNA (Human) - CRH9755 from Cohesion Biosciences
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DNA Polymerase Kappa RNAi - H00051426-R01 from Novus Biologicals
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shRNA set against Human POLK (NM_016218.2) - SHH379260 from Creative biogene
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Human POLK siRNA - orb270257 from Biorbyt
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POLK shRNA Plasmid (Human) - CHH9755 from Cohesion Biosciences
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POLK miRNA 3'UTR clone - MiUTR3H-07857 from Creative biogene
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