RRP8

Gene Symbol RRP8
Entrez Gene 23378
Alt Symbol KIAA0409, NML
Species Human
Gene Type protein-coding
Description ribosomal RNA processing 8, methyltransferase, homolog (yeast)
Other Description RRP8 methyltransferase homolog|cerebral protein 1|nucleomethylin|ribosomal RNA-processing protein 8
Swissprots O43159 Q7KZ78 Q9BVM6
Accessions EAW68691 O43159 AB007869 BAA23705 BC001071 AAH01071 BX417992 D87060 BAB46916 JF432519 ADZ15736 R51624 XM_011519955 XP_011518257 XR_930858 XR_930859 XR_930860 NM_015324 NP_056139
Function Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys- 9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. {ECO:0000269|PubMed:18485871}.
Subcellular Location Nucleus, nucleolus {ECO:0000269|PubMed:11790298, ECO:0000269|PubMed:12429849, ECO:0000269|PubMed:18485871}. Note=Localizes at rDNA locus.

Nucleomethylin Antibody - 4789 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Nucleomethylin Antibody.

RRP8 (K-20) - sc-248500 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-HUCE1 antibody - ab75006 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HUCE1 antibody (ab75006) at 1/500 dilutionLane 1 : extracts from MCF-7 cellsLane 2 : extracts from MCF-7 cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-HUCE1 antibody - ab89478 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-HUCE1 antibody (ab89478) at 1 µg/ml + HeLa cell lysate at 50 µg

Anti-HUCE1 antibody [EPR10645(B)] - ab155989 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10645(B)
All lanes : Anti-HUCE1 antibody [EPR10645(B)] (ab155989) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-RRP8 Antibody (aa89-138) IHC-plus™ - LS-B11561 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-RRP8 Antibody (N-Terminus) - LS-C131113 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RRP8 Antibody (aa406-456) - LS-C290844 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RRP8 Antibody - LS-C308622 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-RRP8 Antibody - LS-C332539 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using RRP8 antibody.

Anti-RRP8 Antibody (aa1-50) - LS-C290841 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RRP8 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RRP8 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-RRP8 Antibody (aa100-149) - LS-C170504 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RRP8 Antibody (aa271-320) - LS-C119843 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from MCF-7 cells, using HUCE1 Antibody. The lane on the right is treated with the synthesized peptide.

HUCE1 Antibody - NBP1-89618 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: HUCE1 Antibody [NBP1-89618] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.

HUCE1 Antibody - NBP1-30507 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: HUCE1 Antibody [NBP1-30507] - Analysis of Cerebral 1 pAb extracts from MCF-7 cells.

HUCE1 Antibody - H00023378-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: HUCE1 Antibody [H00023378-B01P] - Analysis of KIAA0409 expression in HeLa.

Anti-RRP8 antibody produced in rabbit - HPA038487 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RRP8: <B>Cat. No. HPA038487</B>: Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Anti-KIAA0409 (RRP8) (281-295) antibody produced in rabbit - SAB1101505 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-KIAA0409 (RRP8) (281-295) (RB): <B>Cat. No. SAB1101505</B>: Immunofluorescence of HUVEC cells using Anti-KIAA0409 (RRP8) (281-295) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-HUCE1 antibody produced in rabbit - SAB4500977 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-HUCE1: <B>Cat. No. SAB4500977</B>: Immunoblotting analysis of extracts from MCF-7 cells, using Anti-HUCE1 antibody. The lane on the left was treated with the Anti-HUCE1 antibody. The lane on the right (negative control) was treated with both Anti-HUCE1 antibody and the synthesized immunogen peptide.

Anti-KIAA0409 antibody produced in mouse - SAB1407206 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-KIAA0409: <B>Cat. No. SAB1407206</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~50.7 kDa using HeLa lysate.

Anti-KIAA0409 (RRP8) (176-190) antibody produced in rabbit - SAB1101504 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-KIAA0409 (RRP8) (176-190) (RB): <B>Cat. No. SAB1101504</B>: Immunofluorescence of HUVEC cells using Anti-KIAA0409 (RRP8) (176-190) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

RRP8 Antibody - N-terminal region (ARP68566_P050) - ARP68566_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RRP8 Antibody - N-terminal region (ARP68567_P050) - ARP68567_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RRP8 Antibody (OAAF03478) - OAAF03478 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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