DBF4B

Gene Symbol DBF4B
Entrez Gene 80174
Alt Symbol ASKL1, CHIFB, DRF1, ZDBF1B
Species Human
Gene Type protein-coding
Description DBF4 zinc finger B
Other Description ASK-like protein 1|DBF4 homolog B|Dbf4-related factor 1|activator of S-phase kinase-like protein 1|chiffon homolog B|protein DBF4 homolog B|zinc finger, DBF-type containing 1B
Swissprots Q96B19 Q9H912 Q8NFT6 Q8TEX0 D3DX56
Accessions EAW51581 EAW51582 EAW51583 EAW51584 EAW51585 EAW51586 EAW51587 Q8NFT6 AF448801 AAM73808 AF465820 AAL75985 AK023149 BAB14431 AK125771 BAG54245 AK295296 BAG58278 AK303282 BAG64361 AW290902 AY358101 AAQ88468 BC016158 AAH16158 BC026999 BC033660 BM476186 DA475446 XM_006722098 XP_006722161 XM_006722099 XP_006722162 XM_006722100 XP_006722163 XM_006722101 XP_006722164 XM_006722102 XP_006722165 XM_006722104 XP_006722167 XM_006722105 XP_006722168 XM_006722106 XP_006722169 XM_011525277 XP_011523579 XM_011525278 XP_011523580 XM_011525279 XP_011523581 XM_011525280 XP_011523582 XM_011525281 XP_011523583 XM_011525282 XP_011523584 XM_011525283 XP_011523585 XM_011525284 XP_011523586 NM_025104 NP_079380 NM_145663 NP_663696 NR_036623
Function Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. {ECO:0000269|PubMed:12065429, ECO:0000269|PubMed:15668232, ECO:0000269|PubMed:17062569}.
Subcellular Location Nucleus {ECO:0000269|PubMed:12065429, ECO:0000269|PubMed:15668232}. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction.
Tissue Specificity Widely expressed. Highly expressed in testis. {ECO:0000269|PubMed:12065429}.

Dbf4b (D-17) - sc-164150 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dbf4b (D-17): sc-164150. Western blot analysis of Dbf4b expression in Jurkat (A) and MDA-MB-435 (B) whole cell lysates.

Anti-DBF4B antibody - ab69154 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-DBF4B antibody (ab69154) at 1/500 dilutionLane 1 : DBF4B transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-DBF4B antibody - ab119997 from Abcam

Prices $242.00
Sizes 200 µl
Host Goat
Clonality Polyclonal

Anti-DBF4B antibody - N-terminal - ab177911 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-DBF4B antibody - N-terminal (ab177911) at 1 µg/ml + HeLa whole cell lysate at 10 µg

Anti-DBF4B antibody - N-terminal - ab179394 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-DBF4B antibody - N-terminal (ab179394) at 1 µg/ml + NCI-H226 whole cell lysate at 10 µg

Anti-DBF4B / DRF1 Antibody (aa193-207) - LS-C154848 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-DBF4B / DRF1 Antibody - LS-C307892 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DBF4B / DRF1 Antibody (aa201-300) - LS-C307923 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-DBF4B / DRF1 Antibody (N-Terminus) - LS-C186950 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human HeLa. DBF4B antibody dilution 1.0 ug/ml.

Anti-DBF4B / DRF1 Antibody (aa82-131) - LS-C186951 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of NCI-H226 Whole Cell. DCAF10 antibody dilution 1.0 ug/ml.

DBF4B Antibody - NBP2-13900 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DBF4B Antibody - H00080174-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: DBF4B Antibody [H00080174-B01] - analysis of DRF1 expression in transfected 293T cell line by DRF1 MaxPab polyclonal antibody (B01). Lane 1: DRF1 transfected lysate (18.40 KDa). Lane 2: Non-transfected lysate.

DBF4B Antibody (1A7) - H00080174-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
Sandwich ELISA: DBF4B Antibody (1A7) [H00080174-M01] - Detection limit for recombinant GST tagged DBF4B is 1 ng/ml as a capture antibody.

DBF4B Antibody - H00080174-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

DBF4B Antibody - H00080174-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: DBF4B Antibody [H00080174-B01P] - Analysis of DBF4B expression in transfected 293T cell line by DBF4B polyclonal antibody. Lane 1: DRF1 transfected lysate(18.7 KDa). Lane 2: Non-transfected lysate.

DBF4B Polyclonal Antibody - bs-5748R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DBF4B antibody produced in mouse - SAB1401839 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-DBF4B, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1401839</B>: Antibody concentration: 0.1 - 5.0 μg/mL. Specific band of ~18.7 kDa using DBF4B transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-DBF4B antibody produced in rabbit - HPA048465 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DBF4B:<B>Cat. No. HPA048465</B>: Immunohistochemical staining of human rectum shows strong cytoplasmic positivity with a granular pattern in glandular cells.

Monoclonal Anti-DBF4B antibody produced in mouse - SAB1403376 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A7
<B>Immunoblotting</B><BR/>Monoclonal Anti-DBF4B: <B>Cat. No. SAB1403376</B>: Antibody concentration: 1 μg/mL. Specific band of ~37 kDa using immunogen protein lysate.

DBF4B Antibody - N-terminal region (ARP70104_P050) - ARP70104_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DBF4B Antibody - N-terminal region (ARP70105_P050) - ARP70105_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DBF4B-aa193-207-Antibody-internal-region - AF3529a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

DBF4B antibody - GTX120796 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-DBF4B antibody used in ICC/IF. GTX120796

Anti-DBF4B Polyclonal Antibody (OAAI00521) - OAAI00521 from Aviva Systems Biology

Prices $360.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Goat anti-DBF4B (aa193-207) middle region Antibody (OAEB02781) - OAEB02781 from Aviva Systems Biology

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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