SPIRE1
| Gene Symbol | SPIRE1 |
|---|---|
| Entrez Gene | 56907 |
| Alt Symbol | Spir-1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | spire-type actin nucleation factor 1 |
| Other Description | protein spire homolog 1|spire actin nucleation factor 1|spire family actin nucleation factor 1|spire homolog 1 |
| Swissprots | A8K2B5 Q9ULT4 J3KQR5 Q9NQ71 Q1RMD4 J3KQ50 Q8NDP1 Q08AE8 |
| Accessions | EAX01544 EAX01545 EAX01546 Q08AE8 AB032961 BAA86449 AJ277587 CAB96370 AK025433 AK090999 AK290180 BAF82869 AK301711 BAG63182 AL833817 CAD38680 BC016825 AAH16825 BC115005 AAI15006 BC125206 AAI25207 BC125207 AAI25208 DB056921 DB506330 DR001109 XM_005258122 XP_005258179 XM_011525700 XP_011524002 XM_011525701 XP_011524003 XM_011525702 XP_011524004 XM_011525703 XP_011524005 NM_001128626 NP_001122098 NM_001128627 NP_001122099 NM_020148 NP_064533 |
| Function | Acts as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis. {ECO:0000269|PubMed:11747823, ECO:0000269|PubMed:21620703}. |
| Subcellular Location | Cytoplasm, cytoskeleton {ECO:0000269|PubMed:11747823}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:11747823}. Cell membrane {ECO:0000269|PubMed:11747823}; Peripheral membrane protein {ECO:0000269|PubMed:11747823}; Cytoplasmic side {ECO:0000269|PubMed:11747823}. Cytoplasmic vesicle membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion (By similarity). Punctate spots in perinuclear region and cytoplasm, colocalized with Rab11. {ECO:0000250}. |
| Top Pathways | Dorso-ventral axis formation |
Spir-1 CRISPR/Cas9 KO Plasmid (h) - sc-405257 from Santa Cruz Biotechnology
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Spir-1 HDR Plasmid (h) - sc-405257-HDR from Santa Cruz Biotechnology
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Spir-1 Double Nickase Plasmid (h) - sc-405257-NIC from Santa Cruz Biotechnology
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Spir-1 Double Nickase Plasmid (h2) - sc-405257-NIC-2 from Santa Cruz Biotechnology
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Spir-1 CRISPR Activation Plasmid (h) - sc-405257-ACT from Santa Cruz Biotechnology
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Spir-1 CRISPR Activation Plasmid (h2) - sc-405257-ACT-2 from Santa Cruz Biotechnology
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Spir-1 Lentiviral Activation Particles (h) - sc-405257-LAC from Santa Cruz Biotechnology
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Spir-1 Lentiviral Activation Particles (h2) - sc-405257-LAC-2 from Santa Cruz Biotechnology
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Spir-1 siRNA (h) - sc-76567 from Santa Cruz Biotechnology
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Spir-1 shRNA Plasmid (h) - sc-76567-SH from Santa Cruz Biotechnology
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Spir-1 shRNA (h) Lentiviral Particles - sc-76567-V from Santa Cruz Biotechnology
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SPIRE1 RNAi - H00056907-R01 from Novus Biologicals
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MISSION® esiRNA - EHU050371 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20494 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20494 from Sigma-Aldrich
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SPIRE1 siRNA (Human) - CRJ1213 from Cohesion Biosciences
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SPIRE1 - MBS8201191 from MyBioSource
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shRNA set against Human SPIRE1 (NM_020148.2) - SHH416908 from Creative biogene
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shRNA set against Human SPIRE1(NM_020148.2) - SHL029592 from Creative biogene
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SPIRE1 shRNA Plasmid (Human) - CHJ1213 from Cohesion Biosciences
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SPIRE1 miRNA 3'UTR clone - MiUTR1H-09853 from Creative biogene
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