ARHGEF18

Gene Symbol ARHGEF18
Entrez Gene 23370
Alt Symbol P114-RhoGEF
Species Human
Gene Type protein-coding
Description Rho/Rac guanine nucleotide exchange factor (GEF) 18
Other Description 114 kDa Rho-specific guanine nucleotide exchange factor|P114-RHO-GEF|Rho-specific guanine nucleotide exchange factor p114|Rho/Rac guanine nucleotide exchange factor 18|SA-RhoGEF|p114RhoGEF|rho guanine nucleotide exchange factor 18|septin-associated RhoGEF
Swissprots Q6ZSZ5 A8MV62 Q6DD92 O60274 B5ME81
Accessions Q6ZSZ5 AA609742 AA829883 AB011093 BAA25447 AK074372 AK127045 BAC86801 BC008016 BC014994 BC077721 AAH77721 BQ055909 BX404969 DB075637 XM_005272464 XP_005272521 XM_006722705 XP_006722768 XM_006722706 XP_006722769 XM_006722708 XP_006722771 XM_006722709 XP_006722772 XM_011527835 XP_011526137 XM_011527836 XP_011526138 XM_011527837 XP_011526139 XM_011527838 XP_011526140 XM_011527839 XP_011526141 XM_011527840 XP_011526142 XM_011527841 XP_011526143 NM_001130955 NP_001124427 NM_015318 NP_056133
Function Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. {ECO:0000269|PubMed:11085924, ECO:0000269|PubMed:14512443, ECO:0000269|PubMed:15558029}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15558029}. Note=Colocalizes with actin stress fibers.
Tissue Specificity Expressed in all tissues tested with highest expression in kidney and pancreas. Weakly or not expressed in liver, skeletal muscle and testis. {ECO:0000269|PubMed:11085924, ECO:0000269|PubMed:14512443, ECO:0000269|PubMed:15558029}.

ARHGEF18 (Q-14) - sc-169960 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ARHGEF18 (Q-14): sc-169960. Western blot analysis of ARHGEF18 expression in RT-4 (A), HEK293 (B) and HeLa (C) whole cell lysates.

Anti-p114RhoGEF antibody - ab10152 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-p114RhoGEF antibody - ab96520 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab96520 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96520 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-p114RhoGEF antibody [GT1972] - ab184614 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT1972
All lanes : Anti-p114RhoGEF antibody [GT1972] (ab184614) at 1/1000 dilutionLane 1 : Neuro2A whole cell lysate/extractsLane 2 : GL261 whole cell lysate/extractsLane 3 : C8D30 whole cell lysate/extractsLane 4 : NIH-3T3 whole cell lysate/extractsLane 5 : C2C12 whole cell lysate/extractsLysates/proteins at 30 µg per lane.

Anti-p114RhoGEF antibody - N-terminal - ab173626 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-p114RhoGEF antibody - N-terminal (ab173626) at 1/100 dilution + 293 cell line lysate at 35 µg

Anti-ARHGEF18 Antibody IHC-plus™ - LS-B11401 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ARHGEF18 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11401 concentration 20 ug/ml.

Anti-ARHGEF18 Antibody (aa1160-1173) - LS-C55221 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-ARHGEF18 Antibody (aa91-119, APC) - LS-C251678 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 Antibody (aa91-119, AP) - LS-C251677 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 Antibody (aa91-119, Biotin) - LS-C251679 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 Antibody (aa91-119, Azide-free, HRP) - LS-C251680 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 Antibody (aa91-119, FITC) - LS-C251681 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 Antibody (aa91-119, PE) - LS-C251682 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 Antibody (aa90-119) - LS-C319581 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 Antibody (Biotin) - LS-C210720 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Rho guanine nucleotide exchange factor 18 antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 129 kDa. Observed band size: 30 kDa Additional bands at: 60 kDa. We are unsure as to the identity of this extra band.

Anti-ARHGEF18 Antibody (FITC) - LS-C210719 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Rho guanine nucleotide exchange factor 18 antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 129 kDa. Observed band size: 30 kDa Additional bands at: 60 kDa. We are unsure as to the identity of this extra band.

Anti-ARHGEF18 Antibody (aa91-119) - LS-C159109 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ARHGEF18 Antibody western blot of 293 cell line lysates (35 ug/lane). The ARHGEF18 antibody detected the ARHGEF18 protein (arrow).

Anti-ARHGEF18 Antibody (HRP) - LS-C211597 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Rho guanine nucleotide exchange factor 18 antibody at 2 ug/ml. Lane 1: EC109whole cell lysate. Lane 2: 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 129 kDa. Observed band size: 30 kDa Additional bands at: 60 kDa. We are unsure as to the identity of this extra band.

Anti-ARHGEF18 Antibody (aa770-990) - LS-C185475 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of methanol-fixed A431 using ARHGEF18 antibody at 1:200 dilution.

p114RhoGEF Antibody - NBP1-92238 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: p114RhoGEF Antibody [NBP1-92238] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4

p114RhoGEF Antibody - NBP1-32816 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: p114RhoGEF Antibody [NBP1-32816] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE; antibody diluted at 1:1000.

ARHGEF18 Polyclonal Antibody - bs-7144R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF18 antibody produced in rabbit - SAB2700252 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-ARHGEF18: <B>Cat. No. SAB2700252</B>: Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18 antibody at 1:200 dilution.

ANTI-ARHGEF18 (N-TERM) antibody produced in rabbit - SAB1304679 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ARHGEF18 (N-term): <B>Cat. No. SAB1304679</B>: ARHGEF18 Antibody (N-term) western blot analysis in HEK_293 cell line lysates (35 μg/lane).

Anti-ARHGEF18 antibody produced in rabbit - HPA042689 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ARHGEF18: <B>Cat. No. HPA042689</B>: Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
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