RFX3

Gene Symbol RFX3
Entrez Gene 5991
Alt Symbol -
Species Human
Gene Type protein-coding
Description regulatory factor X, 3 (influences HLA class II expression)
Other Description DNA binding protein RFX3|transcription factor RFX3
Swissprots Q95HL5 D3DRH9 Q5JTL7 Q6NW13 Q95HL6 D3DRH8 Q5JTL8 Q8WTU4 P48380 A8K0H5
Accessions EAW58794 EAW58795 EAW58796 EAW58797 EAW58798 EAW58799 P48380 AI811824 AK127804 AK289540 BAF82229 AK310956 AW152407 BC022191 AAH22191 BC067778 AAH67778 BM666287 BU170404 DB036180 DB461398 X76092 CAA53706 XM_006716840 XP_006716903 XM_006716842 XP_006716905 XM_006716843 XP_006716906 XM_006716844 XP_006716907 XM_006716845 XP_006716908 XM_006716847 XP_006716910 XM_011517998 XP_011516300 XM_011517999 XP_011516301 XM_011518000 XP_011516302 XM_011518001 XP_011516303 NM_001282116 NP_001269045 NM_001282117 NP_001269046 NM_002919 NP_002910 NM_134428 NP_602304
Function Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left- right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal c
Subcellular Location Nucleus {ECO:0000305}.

RFX3 (T-17) - sc-10662 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RFX3 (T-17): sc-10662. Western blot analysis of RFX3 expression in HeLa (A) and K-562 (B) nuclear extracts.

RFX3 (T-18) - sc-10663 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RFX3 (T-18): sc-10663. Western blot analysis of RFX3 expression in HeLa (A) and K-562 (B) nuclear extracts and Jurkat (C) whole cell lysate.

Anti-RFX3 antibody - ab92670 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RFX3 antibody (ab92670) at 1/500 dilutionLane 1 : extracts from RAW264.7 cells Lane 2 : extracts from COLO cellsLane 3 : extracts from RAW264.7 cells with immunizing peptide at 5 µgLysates/proteins at 10 µg per lane.

Anti-RFX3 antibody - ab154765 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RFX3 antibody (ab154765) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-RFX3 antibody - ab112962 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RFX3 antibody (ab112962) at 1/100 dilution + MDA-MB435 cell line lysate at 35 µg

Anti-RFX3 antibody - ab67683 from Abcam

Prices $359.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-RFX3 antibody (ab67683) at 1/500 dilutionLane 1 : RFX3 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-RFX3 antibody [3F7] - ab140523 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F7
All lanes : Anti-RFX3 antibody [3F7] (ab140523) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY RFX3 cDNALysates/proteins at 5 µg per lane.

Anti-RFX3 antibody - C-terminal - ab139493 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue labeling RFX3, using ab139493 at 10 µg/ml.

Anti-RFX3 Antibody (aa661-690, AP) - LS-C223878 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (aa661-690, Biotin) - LS-C223880 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (aa661-690, APC) - LS-C223879 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (aa661-690, Azide-free, HRP) - LS-C223881 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (aa661-690, PE) - LS-C223883 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (aa661-690, FITC) - LS-C223882 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (aa660-690) - LS-C324632 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (aa694-739) - LS-C313917 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFX3 Antibody (clone 3F7) - LS-C174579 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFX3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFX3.

Anti-RFX3 Antibody (aa661-690) - LS-C162559 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RFX3 Antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The RFX3 antibody detected the RFX3 protein (arrow).

Anti-RFX3 Antibody (N-Terminus) - LS-C201229 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human HeLa. RFX3 antibody dilution 1.0 ug/ml.

Anti-RFX3 Antibody (aa641-690) IHC-plus™ - LS-B7703 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RFX3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7703 dilution 10 ug/ml.

RFX3 Antibody - NBP1-86301 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RFX3 Antibody [NBP1-86301] - Analysis in control (vector only transfected HEK293T lysate) and rFX3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

RFX3 Antibody - H00005991-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: RFX3 Antibody [H00005991-B01P] - Analysis of RFX3 expression in transfected 293T cell line by RFX3 polyclonal antibody. Lane 1: RFX3 transfected lysate(82.39 KDa). Lane 2: Non-transfected lysate.

RFX3 Antibody - NBP1-31658 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RFX3 Antibody [NBP1-31658] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.

Anti-RFX3 (281-294) antibody produced in rabbit - SAB1100413 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-RFX3 (281-294), <B>Cat. No. SAB1100413</B>: Immunofluorescence of HUVEC cells using Anti-RFX3 (281-294), SAB1100413 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-RFX3, C-Terminal antibody produced in rabbit - SAB4502628 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RFX3: <B>Cat. No. SAB4502628</B>: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-RFX3 antibody. Cells on the left were treated with the Anti-RFX3 antibody. Cells on the right (negative control) were treated with both Anti-RFX3 antibody and the synthesized immunogen peptide.
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