CLNS1A

Gene Symbol CLNS1A
Entrez Gene 1207
Alt Symbol CLCI, CLNS1B, ICln
Species Human
Gene Type protein-coding
Description chloride channel, nucleotide-sensitive, 1A
Other Description chloride channel regulatory protein|chloride channel, nucleotide sensitive 1A|chloride conductance regulatory protein ICln|chloride ion current inducer protein|i(Cln)|methylosome subunit pICln|reticulocyte pICln|reticulocyte protein ICln
Swissprots P54105 Q9NRD3 B2RCS9 Q9NRD2 Q0VDK6
Accessions AAF76858 AAF76859 AAF76861 EAW75032 P54105 AA757599 AF005422 AAB61444 AF026003 AAB88806 AK315259 BAG37676 BC119634 AAI19635 BC119635 AAI19636 BT019907 AAV38710 DA004269 U17899 AAC50111 U53454 AAB03316 X91788 CAA62902 NM_001293 NP_001284
Function Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also indirectly participate in cellular volume control by activation of a swelling-induced chloride conductance pathway. {ECO:0000269|PubMed:10330151, ECO:0000269|PubMed:11713
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:18984161}. Nucleus {ECO:0000269|PubMed:18984161}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:18984161}. Note=A small fraction is also associated with the cytoskeleton.
Top Pathways RNA transport

pICln (C-5) - sc-393525 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pICln (C-5): sc-393525. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

pICln (D-1) - sc-271454 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
plCln (D-1): sc-271454. Western blot analysis of plCln expression in Hep G2 (A) and ECV304 (B) whole cell lysates.

pICln (Q-20) - sc-22128 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
pICln (Q-20): sc-22128. Western blot analysis of pICln expression in Hep G2 whole cell lysate.

pICln (G-1) - sc-271327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
pICln (G-1): sc-271327. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

pICln (FL-237) - sc-32948 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
pICln (FL-237): sc-32948. Western blot analysis of pICln expression in non-transfected 293T: sc-117752 (A), mouse pICln transfected 293T: sc-127333 (B) and Hep G2 (C) whole cell lysates.

pICln (N-20) - sc-22127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
pICln (N-20): sc-22127. Western blot analysis of pICln expression in non-transfected 293T: sc-117752 (A) and mouse pICln transfected 293T: sc-127333 (B) whole cell lysates.

Anti-CLNS1A antibody [2187C2a] - ab53667 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2187C2a
Staining of immunized recombinant protein using anti-CLNS1A antibody [2187C2a](ab53667).

Anti-CLNS1A antibody - C-terminal - ab192907 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CLNS1A antibody - C-terminal (ab192907) at 0.1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : TCMK-1 whole cell lysateLane 5 : NIH3T3 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-CLNS1A antibody - C-terminal - ab196744 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CLNS1A antibody - C-terminal (ab196744) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : Jurkat cell extractLane 3 : K562 cell extractLane 4 : A549 cell extractLane 5 : HuvEc cell extractLane 6 : HeLa cell extractLane 7 : HeLa cell extract with synthesized peptide

Anti-CLNS1A Antibody (aa138-187) - LS-C135258 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CLNS1A antibody LS-C135258 Western blot of Mouse Spleen lysate.

Anti-CLNS1A Antibody (clone 2187C2a) - LS-C184496 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2187C2a
Western blot of immunized recombinant protein using CLNS1A antibody.

Anti-CLNS1A Antibody (C-Terminus) - LS-C99240 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-CLNS1A Antibody (Internal) - LS-C99241 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CLNS1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-CLNS1A Antibody (clone 2187C2a) - LS-C342535 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2187C2a
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CLNS1A mAb.

Anti-CLNS1A Antibody - LS-C79406 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-CLNS1A Antibody (aa184-233) - LS-C291341 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

CLNS1A Antibody - NBP2-33958 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CLNS1A Antibody - NBP2-33964 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CLNS1A Antibody [NBP2-33964] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

CLNS1A Antibody - NBP1-69004 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CLNS1A Antibody [NBP1-69004] - Mouse Spleen lysate, concentration 0.2-1 ug/ml.

CLNS1A Antibody - 26570002 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: CLNS1A Antibody [26570002] - Moderate cytoplasmic and occasional membranous positivity was observed in most normal tissues. Muscle, cells of CNS except Purkinje cells and lymphoid tissues were negative. Malignant tissues displayed in most cases staining patterns similar to the corresponding normal tissue. Image and statement courtesy of the Human Protein Atlas (HPA).

CLNS1A Antibody - NBP2-33959 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: CLNS1A Antibody [NBP2-33959] - Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.

CLNS1A Polyclonal Antibody - bs-9851R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CLNS1A (151-164) antibody produced in rabbit - SAB1104635 from Sigma-Aldrich

Prices $285.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CLNS1A was separated on SDS-PAGE, blotted with Anti-CLNS1A (151-164) (<B>Cat. No. SAB1104635</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + CLNS1A immunizing peptide (human, 151-164)

Anti-CLNS1A antibody produced in rabbit - HPA032045 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CLNS1A: <B>Cat. No. HPA032045</B>: Immunohistochemical staining of Human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Anti-CLNS1A antibody produced in rabbit - HPA031708 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CLNS1A: <B>Cat. No. HPA031708</B>: Immunohistochemical staining of Human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
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