Gene Symbol | AGO3 |
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Entrez Gene | 192669 |
Alt Symbol | EIF2C3 |
Species | Human |
Gene Type | protein-coding |
Description | argonaute RISC catalytic component 3 |
Other Description | argonaute 3|eukaryotic translation initiation factor 2C, 3|hAgo3|protein argonaute-3 |
Swissprots | B1ALI0 Q5TA55 Q9H1U6 Q9H9G7 |
Accessions | EAX07394 EAX07395 Q9H9G7 AA251146 AK022827 BAB14262 AK027796 BAB55374 AK292870 BAF85559 AK302289 BAG63629 AK303929 BAG64857 BC025769 BC066888 AAH66888 BC144409 BC156434 AAI56435 BC172480 AAI72480 BF438817 BF979526 BX104163 XM_005270575 XP_005270632 XM_005270576 XP_005270633 XM_011540879 XP_011539181 XM_011540880 XP_011539182 XM_011540881 XP_011539183 XM_011540882 XP_011539184 NM_024852 NP_079128 NM_177422 NP_803171 |
Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. {ECO:0000255|HAMAP- Rule:MF_03032, ECO:0000269|PubMed:18771919, ECO:0000269|PubMed:23064648}. |
Subcellular Location | Cytoplasm, P-body {ECO:0000255|HAMAP- Rule:MF_03032, ECO:0000269|PubMed:16081698}. |
eIF2C3 CRISPR/Cas9 KO Plasmid (h) - sc-401908 from Santa Cruz Biotechnology
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eIF2C3 HDR Plasmid (h) - sc-401908-HDR from Santa Cruz Biotechnology
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eIF2C3 Double Nickase Plasmid (h) - sc-401908-NIC from Santa Cruz Biotechnology
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eIF2C3 Double Nickase Plasmid (h2) - sc-401908-NIC-2 from Santa Cruz Biotechnology
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eIF2C3 CRISPR Activation Plasmid (h) - sc-401908-ACT from Santa Cruz Biotechnology
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eIF2C3 CRISPR Activation Plasmid (h2) - sc-401908-ACT-2 from Santa Cruz Biotechnology
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eIF2C3 Lentiviral Activation Particles (h) - sc-401908-LAC from Santa Cruz Biotechnology
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eIF2C3 Lentiviral Activation Particles (h2) - sc-401908-LAC-2 from Santa Cruz Biotechnology
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eIF2C3 siRNA (h) - sc-44410 from Santa Cruz Biotechnology
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eIF2C3 shRNA Plasmid (h) - sc-44410-SH from Santa Cruz Biotechnology
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eIF2C3 shRNA (h) Lentiviral Particles - sc-44410-V from Santa Cruz Biotechnology
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Argonaute 3 RNAi - H00192669-R01 from Novus Biologicals
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Argonaute 3 RNAi - H00192669-R02 from Novus Biologicals
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MISSION® esiRNA - EHU101691 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7751 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7751 from Sigma-Aldrich
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AGO3 siRNA (Human) - CRJ6157 from Cohesion Biosciences
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AGO3 - MBS8231669 from MyBioSource
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shRNA set against Human EIF2C3 (NM_177422.2) - SHH284337 from Creative biogene
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AGO3 shRNA Plasmid (Human) - CHJ6157 from Cohesion Biosciences
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EIF2C3 miRNA 3'UTR clone - MiUTR3H-09169 from Creative biogene
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EIF2C3 miRNA 3'UTR clone - MiUTR3H-09170 from Creative biogene
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