NAMPT

Gene Symbol NAMPT
Entrez Gene 10135
Alt Symbol 1110035O14Rik, PBEF, PBEF1, VF, VISFATIN
Species Human
Gene Type protein-coding
Description nicotinamide phosphoribosyltransferase
Other Description NAmPRTase|pre-B cell-enhancing factor|pre-B-cell colony enhancing factor 1|pre-B-cell colony-enhancing factor 1
Swissprots P43490 A4D0Q9 Q3KQV0 A4D0R0 Q8WW95
Accessions AAF19249 AAQ96862 CAM12699 CAN36300 CAV30744 CBX74360 CBX78820 EAL24399 EAL24400 EAW83382 EAW83383 EAW83384 P43490 AI299587 AK023341 AK292851 BAF85540 AK304085 BAH14102 AL832518 CAH56351 BC020691 BC072439 AAH72439 BC106046 AAI06047 BI859562 HQ447548 ADQ32034 L29050 U02020 AAA17884 XM_005250100 XP_005250157 XM_011515707 XP_011514009 NM_005746 NP_005737 NM_182790
Function Catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity). {ECO:0000250|UniProtKB:Q99KQ4, ECO:0000269|PubMed:24130902}.
Subcellular Location Nucleus {ECO:0000269|PubMed:24130902}. Cytoplasm {ECO:0000250|UniProtKB:Q99KQ4}. Secreted {ECO:0000269|PubMed:21741723}. Note=Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum. {ECO:0000269|PubMed:21741723, ECO:0000269|PubMed:24130902}.
Tissue Specificity Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Top Pathways Nicotinate and nicotinamide metabolism

PBEF (E-3) - sc-393444 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBEF (E-3): sc-393444. Western blot analysis of PBEF expression in HL-60 (A), LADMAC (B) and 293T (C) whole cell lysates.

PBEF (F-5) - sc-393443 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBEF (F-5): sc-393443. Western blot analysis of PBEF expression in HL-60 (A), LADMAC (B) and 293T (C) whole cell lysates.

PBEF (F-8) - sc-393510 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBEF (F-8): sc-393510. Western blot analysis of PBEF expression in HL-60 whole cell lysate.

PBEF (H-11) - sc-166946 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBEF (H-11): sc-166946. Western blot analysis of PBEF expression in non-transfected: sc-117752 (A) and mouse PBEF transfected: sc-122402 (B) 293T whole cell lysates.

PBEF (1D3A12) - sc-130058 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBEF (1D3A12): sc-130058. Western blot analysis of PBEF expression in HL-60 whole cell lysate.

PBEF (C-16) - sc-46439 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PBEF (C-20) - sc-46440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PBEF (C-20): sc-46440. Western blot analysis of PBEF expression in HL-60 (A), LADMAC (B) and human PBL (C) whole cell lysates.

PBEF (E-10) - sc-166866 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PBEF (E-10): sc-166866. Western blot analysis of PBEF expression in non-transfected 293T: sc-117752 (A), mouse PBEF transfected 293T: sc-122402 (B) and HL-60 (C) whole cell lysates.

PBEF (H-300) - sc-67020 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PBEF (H-300): sc-67020. Western blot analysis of PBEF expression in HL-60 whole cell lysate.

Anti-Visfatin [14A5] antibody - ab192738 from Abcam

Prices $384.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone 14A5
Immunohistochemical analysis of mouse spleen frozen sections labeling Visfatin with ab192738 at 10 μg/ml (Green) in distinct spleen cell populations (CD11c positive and negative). Secondary Ab: anti-Rat-Alexa488, anti- CD11c-Alexa568.

Anti-Visfatin antibody - ab45890 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Visfatin antibody (ab45890) at 1/250 dilutionLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Visfatin antibody - ab24149 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Visfatin antibody (ab24149) + Human adipose tissue lysateObserved band size : 52 kDa (why is the actual band size different from the predicted?)

Anti-Visfatin antibody - ab58640 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Visfatin antibody (ab58640) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS bufferSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Visfatin antibody - ab184007 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Visfatin antibody (ab184007) at 1/1000 dilution + K562 whole cell lysate at 30 µg

Anti-Visfatin antibody - ab151235 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PBEF with ab151235 at 1/500 dilution. Lower image is co-stained with Hoeschst 33342.

Anti-Visfatin antibody - ab166593 from Abcam

Prices $370.00
Sizes 150 µg
Host Chicken
Clonality Polyclonal

Anti-Visfatin antibody - ab84092 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PBEF by Immunohistochemistry.Sample: FFPE section of Human fetal membrane.Antibody: ab84092 used at a dilution of 1/500.Detection: DAB.

Anti-Visfatin antibody - ab113660 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Visfatin antibody - ab167105 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Visfatin antibody - ab72128 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Visfatin antibody (ab72128) at 1/5000 dilutionLane 1 : Pre immune seraLane 2 : Human skeletal muscle lysateLane 3 : Mouse skeletal muscle lysateLane 4 : Rat skeletal muscle lysate

Anti-Visfatin antibody [1D3A12] - ab66887 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1D3A12
All lanes : Anti-Visfatin antibody [1D3A12] (ab66887) at 1/500 dilutionLane 1 : Truncated PBEF-His recombinant protein (aa338-479;16kDa) Lane 2 : Whole cell lysate from COS-7 cell line transfected with full lenght GFP-PBEF (27kDa+56kDa; aa1-491)

Anti-Visfatin antibody [4D5] - ab71505 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D5
Anti-Visfatin antibody [4D5] (ab71505) at 1/1000 dilution + Whole cell lysate from human HEK 293T cell line at 30 µgSecondaryGoat anti-mouse antibody conjugated to HRPdeveloped using the ECL technique

Anti-Visfatin antibody [4E11C9] - ab59106 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4E11C9
Lane 1 : markerLane 2 : Anti-Visfatin antibody [4E11C9] (ab59106) at 1/200 dilutionLane 1 : As aboveLane 2 : truncated PBEF recombinant protein

Anti-Visfatin antibody (Biotin) - ab113615 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Visfatin antibody (Biotin) - ab166595 from Abcam

Prices $370.00
Sizes 150 µg
Host Chicken
Clonality Polyclonal
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