SF3A3

Gene Symbol SF3A3
Entrez Gene 10946
Alt Symbol PRP9, PRPF9, SAP61, SF3a60
Species Human
Gene Type protein-coding
Description splicing factor 3a, subunit 3, 60kDa
Other Description SAP 61|pre-mRNA splicing factor SF3a (60kD)|spliceosome associated protein 61|spliceosome-associated protein 61|splicing factor 3A subunit 3|splicing factor 3a, subunit 3, 60kD
Swissprots D3DPT5 Q5VT87 Q12874 Q15460
Accessions EAX07304 EAX07305 EAX07306 Q12874 AI492088 AK026887 AK128438 BAG54674 AK222554 BAD96274 AK291181 BAF83870 AK292116 BAF84805 AK301424 BAG62952 BC002395 AAH02395 BC011523 AAH11523 BM702776 BM905122 CA443595 DQ891120 ABM82046 EU176559 ABW03360 N67022 N91991 U08815 AAA19625 XM_005270390 XP_005270447 NM_006802 NP_006793
Function Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Subcellular Location Nucleus speckle.
Tissue Specificity Ubiquitous.
Top Pathways Spliceosome

SAP 61 (A-5) - sc-376266 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SAP 61 (A-5): sc-376266. Western blot analysis of SAP 61 expression in A-431 whole cell lysate.

SAP 61 (C-20) - sc-68786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SAP 61 (H-3) - sc-393673 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SAP 61 (H-3): sc-393673. Western blot analysis of SAP 61 expression in A-431 (A), HeLa (B), Raji (C), 293T (D), Jurkat (E) and NIH/3T3 (F) whole cell lysates.

SAP 61 (G-4) - sc-374464 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SAP 61 (G-4): sc-374464. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

SAP 61 (H-270) - sc-292431 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SAP 61 (H-270): sc-292431. Western blot analysis of SAP 61 expression in HeLa (A), 293T (B) and Raji (C) whole cell lysates.

SAP 61 (N-19) - sc-68788 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 61 (N-19): sc-68788. Western blot analysis of SAP 61 expression in A-431 whole cell lysate.

SAP 61 (P-17) - sc-68789 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 61 (P-17): sc-68789. Western blot analysis of SAP 61 expression in 293T whole cell lysate.

Anti-SF3A3 antibody - ab56823 from Abcam

Prices $370.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal

Anti-SF3A3 antibody [EPR10020] - ab156873 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10020
ab156873 showing +ve staining in Human normal brain.

Anti-SF3A3 antibody [EPR10021(B)] - ab140605 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10021(B)
All lanes : Anti-SF3A3 antibody [EPR10021(B)] (ab140605) at 1/1000 dilutionLane 1 : Fetal liver cell lysateLane 2 : HeLa cell lysateLane 3 : Raji cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SF3A3 / SF3a60 Antibody (aa451-501) - LS-C289241 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SF3A3 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SF3A3 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-SF3A3 / SF3a60 Antibody (aa451-501) - LS-C286915 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse SF3A3 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-SF3A3 used at a dilution of 1:250.

Anti-SF3A3 / SF3a60 Antibody (aa75-125) - LS-C289240 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SF3A3 / SF3a60 Antibody (aa341-390) - LS-C80364 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-SF3A3 / SF3a60 Antibody - LS-C335463 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using SF3A3 antibody.

SF3A3 Antibody - NBP1-87212 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: SF3A3 Antibody [NBP1-87212] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.

SF3A3 Antibody - NBP1-57424 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

SF3A3 Antibody - NBP2-38200 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SF3A3 Antibody [NBP2-38200] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG

SF3A3 Antibody - NBP2-20325 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SF3A3 Antibody [NBP2-20325] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:1000.

SF3A3 Antibody (2E9-2B7) - H00010946-M01A from Novus Biologicals

Prices $349.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 2E9-2B7
Western Blot: SF3A3 Antibody (2E9-2B7) [H00010946-M01A] - SF3A3 monoclonal antibody (M01), clone 2E9-2B7 Western Blot analysis of SF3A3 expression in A-431 ( Cat # L015V1 ).

Anti-SF3A3 antibody produced in rabbit - HPA032054 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SF3A3: <B>Cat. No. HPA032054</B>: Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.

Anti-SF3A3 antibody produced in rabbit - AV40684 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: 721_B_lymphoblast (<B>Cat. No. AV40684</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.25 μg/mL in cell type 721_B_lymphoblast

Monoclonal Anti-SF3A3 antibody produced in mouse - WH0010946M1 from Sigma-Aldrich

Prices $347.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 2E9-2B7
<B>Immunoblotting</B><BR/>Monoclonal Anti-SF3A3, <B>Cat. No. WH0010946M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~58.8 kDa using A-431 lysate.

Anti-SF3A3 antibody produced in rabbit - SAB2102121 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SF3A3: <B>Cat. No.SAB2102121</B>: SF3A3 in Fetal Muscle tissue lysate with SF3A3 antibody at 1.0 μg/mL.

SF3A3 antibody - middle region (ARP40684_P050) - ARP40684_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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