PHF1

Gene Symbol	PHF1
Entrez Gene	5252
Alt Symbol	MTF2L2, PCL1, PHF2, TDRD19C, hPHF1
Species	Human
Gene Type	protein-coding
Description	PHD finger protein 1
Other Description	hPCl1\|polycomb-like protein 1\|tudor domain containing 19C
Swissprots	O60929 Q5SU08 B1AZX2 O43189 B1AZX3 Q5SU07 Q96KM7
Accessions	CAA16158 CAA16159 CAC38366 CAC38367 EAX03729 EAX03730 O43189 AF029678 AAC52062 AF052205 AAC13273 AK225913 AK295412 BAG58363 AK299162 BAG61210 AK316499 BAH14870 BC008834 AAH08834 CB242267 DA065298 DA872118 DQ896419 ABM87418 EU176431 ABW03882 XM_011514662 XP_011512964 XM_011514663 XP_011512965 XM_011514664 XP_011512966 XM_011514665 XP_011512967 XM_011514666 XP_011512968 XM_011514667 XP_011512969 XM_011514668 XP_011512970 XM_011514669 XP_011512971 XM_011514670 XP_011512972 XM_011514671 XP_011512973 XM_011548442 XP_011546744 XM_011548443 XP_011546745 XM_011548444 XP_011546746 XM_011548445 XP_011546747 XM_011548446 XP_011546748 XM_011548447 XP_011546749 XM_011548448 XP_011546750 XM_011548449 XP_011546751 XM_011548450 XP_011546752 XM_011548451 XP_011546753 XR_926249 XR_926250 XR_953080 XR_953081 NM_002636 NP_002627 NM_024165 NP_077084 NR_027692
Function	Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18285464 and PubMed:23273982). According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2 (PubMed:23142980). Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53. {ECO:0000269\|PubMed:18086877, ECO:0000269\|PubMed:18285464, ECO:0000269\|Pu
Subcellular Location	Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Co- localizes with NEK6 in the centrosome.
Tissue Specificity	Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.

PHF1 (H-40) - sc-98763 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

PHF1 (P-12) - sc-107933 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

PHF1 (P-12): sc-107933. Western blot analysis of PHF1 expression in A-431 (A) and HeLa (B) nuclear extracts.

PHF1 (S-14) - sc-107934 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

PHF1 (SK-J6) - sc-101107 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

PHF1 (SK-J6): sc-101107. Western blot analysis of PHF1 expression in A-431 whole cell lysate.

PHF1 (864C6a) - sc-130646 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

PHF1 (864C6a): sc-130646. Western blot analysis of human recombinant PHF1 fusion protein.

Anti-PHF1 antibody - ab70094 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-PHF1 antibody (ab70094) at 1/500 dilutionLane 1 : HeLa cell extractsLane 2 : HeLa cell extracts with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-PHF1 antibody [864C6a] - ab70728 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	864C6a

Anti-PHF1 antibody [864C6a] (ab70728) + immunising recombinant protein

Anti-PHF1 antibody [EPR14222] - ab184951 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR14222

All lanes : Anti-PHF1 antibody [EPR14222] (ab184951) at 1/20000 dilutionLane 1 : 293 lysateLane 2 : Jurkat lysateLane 3 : MCF7 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PHF1 Antibody - LS-C74823 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal

Anti-PHF1 Antibody (C-Terminus) - LS-C144885 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PHF1 Antibody (clone 864C6a) - LS-C184669 from LifeSpan Bioscience

Prices	$335.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	864C6a

Anti-PHF1 Antibody (Lys381) - LS-C192031 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PHF1 Antibody (aa421-470) - LS-C31286 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PHF1 Antibody (N-Terminus) - LS-C80269 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PHF1 Antibody (C-Terminus) - LS-C80270 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

NTERA2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PHF1 Antibody (aa345-394) - LS-C118676 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from HeLa cells, using PHF1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-PHF1 Antibody (clone 2D3) - LS-C197891 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2D3

PHF1 monoclonal antibody (M01), clone 2D3 Western Blot analysis of PHF1 expression in A-431.

Anti-PHF1 Antibody (clone 864C6a) - LS-C342699 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	864C6a

Left : Western blot analysis of immunized recombinant protein, using anti-PHF1 monoclonal antibody. Right : CBB staining of immunized recombinant protein.

Anti-PHF1 Antibody (aa164-197) - LS-C344267 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Western blot of lysate from mouse testis tissue lysate with PHF1 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

PHF1 Antibody (2G7) - H00005252-M04 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2G7

Western Blot: PHF1 Antibody (2G7) [H00005252-M04] - Analysis of PHF1 expression in PC-12 (Cat # L012V1).

PHF2 Antibody - NBP1-83080 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: PHF2 Antibody [NBP1-83080] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.

PHF1 Antibody - NBP1-82613 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PHF1 Antibody [NBP1-82613] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)

PHF2 Antibody - NB300-162 from Novus Biologicals

Prices	$229.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: PHF2 Antibody [NB300-162] - Analysis of PHF2 in placenta lysate using this antibody at 1:500 dilution.

PHF1 Antibody (4F5) - H00005252-M05 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4F5

Western Blot: PHF1 Antibody (4F5) [H00005252-M05] - Western Blot analysis of PHF1 expression in A-431 ( Cat # L015V1 ).

PHF1 Antibody (4D8) - H00005252-M02 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4D8

Western Blot: PHF1 Antibody (4D8) [H00005252-M02] - PHF1 monoclonal antibody (M02), clone 4D8 Analysis of PHF1 expression in K-562.

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