ARHGAP35

Gene Symbol ARHGAP35
Entrez Gene 2909
Alt Symbol GRF-1, GRLF1, P190-A, P190A, p190ARhoGAP, p190RhoGAP
Species Human
Gene Type protein-coding
Description Rho GTPase activating protein 35
Other Description glucocorticoid receptor DNA binding factor 1|glucocorticoid receptor DNA-binding factor 1|glucocorticoid receptor repression factor 1|rho GAP p190A|rho GTPase-activating protein 35
Swissprots Q9C0E1 A7E2A4 Q14452 Q9NRY4
Accessions EAW57450 EAW57451 EAW57452 EAW57453 Q9NRY4 AB051509 BAB21813 AF159851 AAF80386 AK096123 AK097314 AK298356 BAG60600 AL831914 BC003514 BC065491 BC131565 AAI31566 BC150257 AAI50258 BE871213 BM855708 CA429690 CX163309 M73077 AAA58618 XM_011526873 XP_011525175 XR_935811 NM_004491 NP_004482 NM_024342
Function Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'- GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor. {ECO:0000269|PubMed:1894621}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
Top Pathways Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

p190-A RhoGAP (D8Q6C) Rabbit mAb - 12164 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8Q6C
Western blot analysis of extracts from various cell lines using p190-A RhoGAP (D8Q6C) Rabbit mAb.

p190-A RhoGAP Antibody - 2513 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using p190-A RhoGAP Antibody.

p190-A RhoGAP (C59F7) Rabbit mAb - 2860 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C59F7
Western blot analysis of extracts from various cell types using p190-A RhoGAP (C59F7) Rabbit mAb.

GRF-1 (N-13) - sc-131536 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GRF-1 (N-13): sc-131536. Western blot analysis of GRF-1 expression in c4 whole cell lysate (A) and MCF7 nuclear extract (B).

GRF-1 (C-14) - sc-131534 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

GRF-1 (H-200) - sc-367517 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRF-1 (H-200): sc-367517. Western blot analysis of GRF-1 expression in c4 whole cell lysate.

GRF-1 (2389D3a) - sc-81094 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
GRF-1 (2389D3a): sc-81094. Western blot analysis of human recombinant GRLF1 fusion protein.

Anti-GRLF1 (phospho Y1087) antibody - ab203274 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling GRLF1 with ab203274 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining

Anti-GRLF1 (phospho Y1087) antibody - ab138355 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-GRLF1 (phospho Y1087) antibody (ab138355) at 1/500 dilution + K562 cell extract at 30 µg

Anti-GRLF1 (phospho Y1105) antibody - ab182636 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRLF1 (phospho Y1105) antibody (ab182636) at 1/500 dilutionLane 1 : Mouse brain tissue lysate with immunizing peptideLane 2 : Mouse brain tissue lysate

Anti-GRLF1 (phospho Y1105) antibody - ab195833 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRLF1 (phospho Y1105) antibody (ab195833) at 1/500 dilutionLane 1 : EGF treated 293 whole cell lysate with antigen-specific peptideLane 2 : EGF treated 293 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-GRLF1 (phospho Y1105) antibody - ab55339 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab55339, at 1/50 dilution, staining GRLF1 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising phosphopeptide.

Anti-GRLF1 antibody - ab85950 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRLF1 antibody (ab85950) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-GRLF1 antibody - ab134726 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-GRLF1 antibody (ab134726) at 1/500 dilution + MCF7 cell lysate at 30 µg

Anti-GRLF1 antibody [2389D3a] - ab58674 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2389D3a
Anti-GRLF1 antibody [2389D3a] (ab58674) + immunising recombinant protein

Anti-ARHGAP35 / GRLF1 Antibody - LS-C74014 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGAP35 / GRLF1 Antibody - LS-C66598 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-ARHGAP35 / GRLF1 Antibody (aa1200-1250) - LS-C125692 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGAP35 / GRLF1 Antibody (clone 2389D3a) - LS-C184572 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2389D3a

Anti-ARHGAP35 / GRLF1 Antibody (phospho-Tyr1087) - LS-C291499 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ARHGAP35 / GRLF1 Antibody (aa1071-1120) - LS-C199022 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain tissue, using GRF-1 Antibody. The sample on the right was incubated with synthetic peptide.

Anti-ARHGAP35 / GRLF1 Antibody (clone 2389D3a) - LS-C342854 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2389D3a
Western blot analysis of immunized recombinant protein, using anti-GRLF1 monoclonal antibody.

Anti-ARHGAP35 / GRLF1 Antibody (clone 4D4-F8) - LS-C197357 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D4-F8
Detection limit for recombinant GST tagged GRLF1 is 0.1 ng/ml as a capture antibody.

Anti-ARHGAP35 / GRLF1 Antibody (phospho-Tyr1087) - LS-C178061 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of p-CD318 (Y707) pAb in extracts from K562 cells.

Anti-ARHGAP35 / GRLF1 Antibody (aa1032-1081) - LS-C199874 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from MCF7 cells, using GRF-1 antibody.
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