| Gene Symbol | MGAT5B |
|---|---|
| Entrez Gene | 146664 |
| Alt Symbol | GnT-IX, GnT-VB |
| Species | Human |
| Gene Type | protein-coding |
| Description | mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B |
| Other Description | N-acetylglucosaminyl-transferase Vb|N-acetylglucosaminyltransferase IX|alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B|alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase B|beta(1,6)-N-acetylglucosaminyltransferase V|glcNAc-T Vb|hGnTVb|mannoside acetylglucosaminyltransferase 5B|mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isoenzyme B |
| Swissprots | Q76D04 Q96LS2 Q766X5 Q6P3S8 Q6P6B3 Q3V5L5 |
| Accessions | EAW89457 EAW89458 EAW89459 EAW89460 Q3V5L5 AB109185 BAC84969 AB114297 BAD02406 AB235153 BAE44474 AK123189 AW191036 BC036948 BC041577 BC062354 AAH62354 BC063862 AAH63862 BC144361 BC156823 AAI56824 HG509473 HG509474 HG509475 XM_005257065 XP_005257122 XM_006721707 XP_006721770 XM_011524348 XP_011522650 XM_011524349 XP_011522651 XM_011524350 XP_011522652 XM_011524351 XP_011522653 XM_011524352 XP_011522654 XM_011524353 XP_011522655 XM_011524354 XP_011522656 XR_934377 NM_001199172 NP_001186101 NM_144677 NP_653278 NM_198955 NP_945193 |
| Function | Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N- acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAcbeta1,2-Manalpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan. Also acts on the GlcNAcbeta1,2-Manalpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan. Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway. {ECO:0000269|PubMed:12941944, ECO:0000269|PubMed:14617637, ECO:0000269|PubMed:14623122, ECO:0000269|PubMed:16606368, ECO:0000269|PubMed:16857188}. |
| Subcellular Location | Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. |
| Tissue Specificity | Predominantly expressed in brain. Expressed in all area of the adult and fetal brain Also expressed at much lower level in testis, spleen and thymus. {ECO:0000269|PubMed:12941944, ECO:0000269|PubMed:14623122}. |
| Top Pathways | N-Glycan biosynthesis, Other types of O-glycan biosynthesis |
GnT-VB CRISPR/Cas9 KO Plasmid (h) - sc-414584 from Santa Cruz Biotechnology
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GnT-VB HDR Plasmid (h) - sc-414584-HDR from Santa Cruz Biotechnology
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GnT-VB Double Nickase Plasmid (h) - sc-414584-NIC from Santa Cruz Biotechnology
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GnT-VB Double Nickase Plasmid (h2) - sc-414584-NIC-2 from Santa Cruz Biotechnology
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GnT-VB CRISPR Activation Plasmid (h) - sc-414584-ACT from Santa Cruz Biotechnology
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GnT-VB CRISPR Activation Plasmid (h2) - sc-414584-ACT-2 from Santa Cruz Biotechnology
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GnT-VB Lentiviral Activation Particles (h) - sc-414584-LAC from Santa Cruz Biotechnology
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GnT-VB Lentiviral Activation Particles (h2) - sc-414584-LAC-2 from Santa Cruz Biotechnology
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GnT-VB siRNA (h) - sc-94051 from Santa Cruz Biotechnology
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GnT-VB shRNA Plasmid (h) - sc-94051-SH from Santa Cruz Biotechnology
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GnT-VB shRNA (h) Lentiviral Particles - sc-94051-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU030631 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN13279 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND13279 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR09674 from Sigma-Aldrich
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MGAT5B siRNA (Human) - CRJ5481 from Cohesion Biosciences
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shRNA set against Human Mgat5b (NM_144677.2) - SHH341423 from Creative biogene
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shRNA set against Human MGAT5B(NM_144677.2) - SHH187535 from Creative biogene
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shRNA set against Human MGAT5B(NM_198955.1) - SHH187553 from Creative biogene
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MGAT5B shRNA Plasmid (Human) - CHJ5481 from Cohesion Biosciences
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MGAT5B miRNA 3'UTR clone - MiUTR1H-06246 from Creative biogene
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MGAT5B miRNA 3'UTR clone - MiUTR1H-06247 from Creative biogene
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