MAGEA3

Gene Symbol MAGEA3
Entrez Gene 4102
Alt Symbol CT1.3, HIP8, HYPD, MAGE3, MAGEA6
Species Human
Gene Type protein-coding
Description melanoma antigen family A3
Other Description MAGE-3 antigen|antigen MZ2-D|cancer/testis antigen 1.3|cancer/testis antigen family 1, member 3|melanoma antigen family A, 3|melanoma-associated antigen 3
Swissprots Q6FHI6 P43357
Accessions AAA17446 P43357 AK223519 BAD97239 AK292384 BAF85073 BC000340 AAH00340 BC005963 AAH05963 BC011744 AAH11744 BC016803 AAH16803 BC017389 AAH17389 CR541767 CAG46566 CR541768 CAG46567 CR541774 CAG46573 CR542020 CAG46817 DQ891850 ABM82776 DQ895040 ABM85966 XM_005274676 XP_005274733 XM_006724818 XP_006724881 XM_011531160 XP_011529462 XM_011531161 XP_011529463 NM_005362 NP_005353
Function Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. {ECO:0000269|PubMed:20864041}.
Tissue Specificity Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.

MAGE-A3/6 (F-14) - sc-33233 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MAGE-A3/6 (C-13) - sc-33234 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAGE-A3/6 (C-13): sc-33234. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts and Leydig cells.

MAGE-A3/6 (H-42) - sc-367226 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

MAGE-A3/6 (G-23) - sc-130810 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
MAGE-A3/6 (G-23): sc-130810. Western blot analysis of MAGE-A3/6 expression in A549 whole cell lysate.

Anti-MAGEA3 antibody - ab38496 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab38496 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38496 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-MAGEA3 antibody - ab115332 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab115332 at 10ug/ml staining MAGEA3 in Human testis tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-MAGEA3 antibody [1H1] - ab140678 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H1
All lanes : Anti-MAGEA3 antibody [1H1] (ab140678) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MAGEA3 cDNALysates/proteins at 5 µg per lane.

Anti-MAGEA3 antibody [2B1] - ab140789 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B1
All lanes : Anti-MAGEA3 antibody [2B1] (ab140789) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY MAGEA3 cDNALysates/proteins at 5 µg per lane.

Anti-MAGEA3 antibody - C-terminal - ab199152 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MAGEA3 with ab199152 at 1/50 dilution.

Anti-MAGEA3 Antibody - LS-C308854 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MAGEA3 Antibody (clone 2F10) - LS-C175476 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F10
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-MAGEA3 mouse monoclonal antibody.

Anti-MAGEA3 Antibody - LS-C311349 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MAGEA3 Antibody (clone 2E11) - LS-C175505 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E11
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA3.

Anti-MAGEA3 Antibody - LS-C311351 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MAGEA3 Antibody (clone 3B2) - LS-C175483 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3B2
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAGEA3 mouse monoclonal antibody.

Anti-MAGEA3 Antibody (aa285-314) - LS-C322801 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAGEA3 Antibody (aa298-314) IHC-plus™ - LS-B884 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MAGEA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B884 concentration 10 ug/ml.

Anti-MAGEA3 Antibody (clone 4E2) IHC-plus™ - LS-B10016 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 4E2
Anti-MAGEA3 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10016 concentration 10 ug/ml.

Anti-MAGEA3 Antibody (clone 6D10) - LS-C197641 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D10
Western Blot analysis of MAGEA3 expression in transfected 293T cell line by MAGEA3 monoclonal antibody (M01), clone 6D10.Lane 1: MAGEA3 transfected lysate (Predicted MW: 34.7 KDa).Lane 2: Non-transfected lysate.

Anti-MAGEA3 Antibody (clone 1G9) IHC-plus™ - LS-B11677 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G9
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-MAGEA3 Antibody (clone 1A9) - LS-C337054 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A9
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAGEA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-MAGEA3 Antibody - LS-C89504 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MAGEA3 Antibody (clone 24B6) - LS-C175485 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 24B6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA3.

Anti-MAGEA3 Antibody (aa285-314, AP) - LS-C237356 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MAGEA3 Antibody (aa285-314, APC) - LS-C237357 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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