ID3

Gene Symbol ID3
Entrez Gene 3399
Alt Symbol HEIR-1, bHLHb25
Species Human
Gene Type protein-coding
Description inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Other Description DNA-binding protein inhibitor ID-3|ID-like protein inhibitor HLH 1R21|class B basic helix-loop-helix protein 25|helix-loop-helix protein HEIR-1|inhibitor of differentiation 3
Swissprots Q02535 O75641 A8K1T8
Accessions CAA51827 EAW95060 EAW95061 Q02535 A17546 CAA01342 A17548 CAA01343 AK225965 AK290003 BAF82692 BC003107 AAH03107 BM921819 BP193075 BT006791 AAP35437 D28449 BAA05815 DQ892504 ABM83430 DQ895539 ABM86465 DQ895717 ABM86643 X66924 CAA47360 X69111 CAA48862 NM_002167 NP_002158
Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. {ECO:0000269|PubMed:8437843}.
Subcellular Location Nucleus.
Tissue Specificity Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Top Pathways TGF-beta signaling pathway, Signaling pathways regulating pluripotency of stem cells

ID3 (D16D10) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 12038 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D16D10
Flow cytometric analysis of Ramos cells using ID3 (D16D10) Rabbit mAb (Alexa Fluor® 647 Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red).

ID3 (D16D10) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 11997 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D16D10
Flow cytometric analysis of Ramos cells using ID3 (D16D10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red).

ID3 (D16D10) Rabbit mAb - 9837 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16D10
Western blot analysis of extracts from various cell lines using ID3 (D16D10) Rabbit mAb.

Id3 (H-70) - sc-13046 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Id3 (C-20) - sc-490 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Id3 (C-20): sc-490. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Id3 (2B11) - sc-56712 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Id3 (4i234) - sc-71311 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-ID3 antibody - ab41834 from Abcam

Prices $378.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Ab41834 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Anti-ID3 antibody - ab55269 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ID3 antibody - ab86017 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ID3 antibody (ab86017) at 1/1000 dilutionLane 1 : Uninduced (negative control) culture of E.coliLane 2 : Induced culture of E.coliSecondaryAnti-Rabbit IgG HRP conjugated at 1/1000 dilution

Anti-ID3 antibody - ab131712 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ID3 antibody [10D3] - ab118032 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10D3
ab118032 staining ID3 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab118032 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouset polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. ID3 expression can be observed in Muller cells.See Abreview

Anti-ID3 antibody [2B11] - ab36501 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2B11
Overlay histogram showing HeLa cells stained with ab36501 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36501, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ID3 antibody, prediluted - ab41835 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Ab41835 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Anti-ID3 Antibody (clone 5C7) - LS-C114798 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5C7
IHC of paraffin-embedded thyroid tissue using anti-ID3 mouse monoclonal antibody. (Dilution 1:50).

Anti-ID3 Antibody (clone 15F4) - LS-C114800 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 15F4
IHC of paraffin-embedded pancreas tissue using anti-ID3 mouse monoclonal antibody. (Dilution 1:50).

Anti-ID3 Antibody (clone 15E3) - LS-C114835 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 15E3
IHC of paraffin-embedded prostate tissue using anti-ID3 mouse monoclonal antibody. (Dilution 1:50).

Anti-ID3 Antibody (aa1-119, clone 2B11) - LS-C20009 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B11

Anti-ID3 Antibody (aa1-119) - LS-C83533 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-ID3 Antibody (N-Terminus) - LS-C30560 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T Cell Lysate

Anti-ID3 Antibody (aa58-107) - LS-C146079 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ID3 antibody LS-C146079 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml.

Anti-ID3 Antibody (clone 4D1) - LS-C114799 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4D1
Anti-ID3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3.

Anti-ID3 Antibody (N-Terminus) - LS-C146382 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ID3 antibody LS-C146382 Western blot of 293T Cell lysate. Antibody concentration 1 ug/ml.

Anti-ID3 Antibody (aa86-115, AP) - LS-C222830 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ID3 Antibody (aa86-115, APC) - LS-C222831 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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