PHLPP2

Gene Symbol PHLPP2
Entrez Gene 23035
Alt Symbol PHLPPL
Species Human
Gene Type protein-coding
Description PH domain and leucine rich repeat protein phosphatase 2
Other Description PH domain leucine-rich repeat-containing protein phosphatase 2
Swissprots Q9Y2E3 Q9NV17 A1L374 Q6ZVD8
Accessions EAW59225 EAW59226 Q6ZVD8 AB023148 BAA76775 AK001661 AK001854 BAA91943 AK124678 BAC85924 AM393067 CAL37945 AM393542 CAL38419 BC035267 AAH35267 BC129927 AAI29928 BC129983 AAI29984 BX647823 BX648157 BX648394 NM_001289003 NP_001275932 NM_015020 NP_055835
Function Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser- 657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. {ECO:0000269|PubMed:17386267, ECO:0000269|PubMed:18162466, ECO:0000269|PubMed:19079341}.
Subcellular Location Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Note=In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
Tissue Specificity In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). {ECO:0000269|PubMed:19079341}.
Top Pathways PI3K-Akt signaling pathway

PHLPPL (T-17) - sc-137663 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PHLPPL (T-17): sc-137663. Western blot analysis of PHLPPL expression in HCT 116 (A) and COLO 320DM (B) whole cell lysates.

PHLPPL (Y-14) - sc-137664 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PHLPPL (Y-14): sc-137664. Western blot analysis of PHLPPL expression in HeLa (A), HEK293 (B) and K-562 (C) whole cell lysates.

Anti-PHLPP2 antibody - ab71973 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PHLPP2 antibody (ab71973) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg

Anti-PHLPP2 antibody - ab62830 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-PHLPP2 antibody (ab62830) at 0.1 µg/ml + Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate at 35 µg

Anti-PHLPP2 antibody - ab77665 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin fixed and paraffin embedded human colon carcinoma tissue stained with ab77665, at a 1/10 dilution, followed by treatment with a peroxidase conjugated secondary antibody and DAB staining.

Anti-PHLPP2 antibody - N-terminal - ab153918 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PHLPP2 with ab153918 at 1/500 dilution. Lower image is co-stained with Hoechst 33342.

Anti-PHLPP2 / PHLPPL Antibody (C-Terminus) - LS-C287530 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PHLPP2 / PHLPPL Antibody (C-Terminus) IHC-plus™ - LS-B6340 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-PHLPP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6340 dilution 1:50.

Anti-PHLPP2 / PHLPPL Antibody (aa4-31) - LS-C97139 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PHLPP2 Antibody western blot of HL-60 cell line lysates (15 ug/lane). The PHLPP2 antibody detected the PHLPP2 protein (arrow).

Anti-PHLPP2 / PHLPPL Antibody (aa1307-1320) - LS-C55275 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.1 ug/ml) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PHLPP2 / PHLPPL Antibody (aa1295-1323) - LS-C163578 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PHLPP2 Antibody IHC of formalin-fixed and paraffin-embedded colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PHLPP2 / PHLPPL Antibody (clone SP220) - LS-C210438 from LifeSpan Bioscience

Prices $530.00
Sizes 500 µl
Host Rabbit
Clonality Monoclonal
Clone SP220
Immunohistochemistry of Human Normal Prostate stained with anti-PHLLP2 antibody

Anti-PHLPP2 / PHLPPL Antibody (C-Terminus) IHC-plus™ - LS-B8861 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PHLPP2 / PHLPPL antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PHLPP2 Antibody - NB100-1812 from Novus Biologicals

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PHLPP2 Antibody [NB100-1812] - Detection of Human and Mouse PHLPPL on HeLa whole cell lysate using NB100-1812. PHLPPL was also IPed, albeit less efficiently, using another rabbit anti-PHLPPL antibody.

PHLPP2 Antibody - NBP2-19803 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PHLPP2 Antibody - NB100-61668 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

PHLPP2 Antibody - 38180002 from Novus Biologicals

Prices $299.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ELISA: PHLPP2 Antibody [38180002]

Anti-PHLPP2 antibody produced in goat - SAB2500786 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-PHLPP2: <B>Cat. No. SAB2500786</B>: (0.1 μg/mL) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PHLPP2, Rabbit Monoclonal Antibody - SAB5500161 from Sigma-Aldrich

Prices $247.00
Sizes 100 µl
Clonality Monoclonal
Clone SP220
<B>Immunohistochemistry</B><BR/>Anti-PHLPP2: <B>Cat. No. SAB5500161</B>: Immunohistochemistry of PHLPP2 in Human prostate adenocarcinoma tissue with PHLPP2 antibody.

Anti-PHLPP2 (N-terminal) antibody produced in rabbit - SAB2701381 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-PHLPP2 (N-terminal): <B>Cat. No. SAB2701381</B>: Immunofluorescence analysis of paraformaldehyde-fixed A431, using PHLPP2 antibody at 1:200 dilution.

Anti-PHLPP2 antibody produced in rabbit - HPA048598 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PHLPP2:<B>Cat. No. HPA048598</B>: Immunohistochemical staining of human spleen shows strong positivity in a subset of cells in the red pulp.

Anti-PHLPP2 antibody produced in rabbit - SAB3501016 from Sigma-Aldrich

Prices $341.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>PHLPP2 Antibody: <B>Cat. No. SAB3501016</B>: Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 μg/mL in (Lane A) the presence and (Lane B) the absence of blocking peptide.

PHLPP2-Antibody-C-term - AP7799b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of PHLPP2 Antibody (C-term) (Cat. #AP7799b) in MDA-MB231 cell line lysates (35ug/lane).PHLPP2 (arrow) was detected using the purified Pab.

PHLPP2-Antibody-N-term - AP7799a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
PHLPP2 Antibody (N-term) (Cat. #AP7799a) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the PHLPP2 antibody detected the PHLPP2 protein (arrow).

PHLPP2-Antibody - AF1821a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF1821a (0.1 µg/ml) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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