Gene Symbol | SETDB1 |
---|---|
Entrez Gene | 9869 |
Alt Symbol | ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21 |
Species | Human |
Gene Type | protein-coding |
Description | SET domain, bifurcated 1 |
Other Description | ERG-associated protein with SET domain|ERG-associated protein with a SET domain, ESET|H3-K9-HMTase 4|histone H3-K9 methyltransferase 4|histone-lysine N-methyltransferase SETDB1|histone-lysine N-methyltransferase, H3lysine-9 specific 4|lysine N-methyltransferase 1E|tudor domain containing 21 |
Swissprots | Q96GM9 Q5SZE0 A6NEW2 Q5SZD9 Q15047 Q5SZE7 Q5SZD8 |
Accessions | CBJ06473 CBJ06474 EAW53503 EAW53504 EAW53505 EAW53506 EAW53507 EAW53508 Q15047 AI582266 AK095075 AK302461 BAG63752 AK308335 BC009362 AAH09362 BC028671 AAH28671 D31891 BAA06689 DA418593 DB062762 XM_005245641 XP_005245698 NM_001145415 NP_001138887 NM_001243491 NP_001230420 NM_012432 NP_036564 |
Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. {ECO:0000269|PubMed:12869583, ECO:0000269|PubMed:14536086, ECO:0000269|PubMed:15327775, ECO:0000269|PubMed:17952062}. |
Subcellular Location | Nucleus. Chromosome. Note=Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin. |
Tissue Specificity | Widely expressed. High expression in testis. |
Top Pathways | Signaling pathways regulating pluripotency of stem cells, Lysine degradation |
ESET CRISPR/Cas9 KO Plasmid (h) - sc-401325 from Santa Cruz Biotechnology
|
||||||||
ESET HDR Plasmid (h) - sc-401325-HDR from Santa Cruz Biotechnology
|
||||||||
ESET Double Nickase Plasmid (h) - sc-401325-NIC from Santa Cruz Biotechnology
|
||||||||
ESET Double Nickase Plasmid (h2) - sc-401325-NIC-2 from Santa Cruz Biotechnology
|
||||||||
ESET CRISPR Activation Plasmid (h) - sc-401325-ACT from Santa Cruz Biotechnology
|
||||||||
ESET CRISPR Activation Plasmid (h2) - sc-401325-ACT-2 from Santa Cruz Biotechnology
|
||||||||
ESET Lentiviral Activation Particles (h) - sc-401325-LAC from Santa Cruz Biotechnology
|
||||||||
ESET Lentiviral Activation Particles (h2) - sc-401325-LAC-2 from Santa Cruz Biotechnology
|
||||||||
ESET siRNA (h) - sc-45659 from Santa Cruz Biotechnology
|
||||||||
ESET shRNA Plasmid (h) - sc-45659-SH from Santa Cruz Biotechnology
|
||||||||
ESET shRNA (h) Lentiviral Particles - sc-45659-V from Santa Cruz Biotechnology
|
||||||||
SETDB1 RNAi - H00009869-R01 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU046261 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN19174 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND19174 from Sigma-Aldrich
|
||||||||
SETDB1 siRNA (Human) - CRH6618 from Cohesion Biosciences
|
||||||||
SETDB1 - MBS8202235 from MyBioSource
|
||||||||
SETDB1 shRNA Plasmid (Human) - CHH6618 from Cohesion Biosciences
|
||||||||
SETDB1 miRNA 3'UTR clone - MiUTR3H-06768 from Creative biogene
|
||||||||
SETDB1 miRNA 3'UTR clone - MiUTR3H-06769 from Creative biogene
|