NPLOC4

Gene Symbol NPLOC4
Entrez Gene 55666
Alt Symbol NPL4
Species Human
Gene Type protein-coding
Description nuclear protein localization 4 homolog (S. cerevisiae)
Other Description nuclear protein localization protein 4 homolog
Swissprots Q8N3J1 Q9NWR5 Q9H8V2 Q8TAT6 Q9P229 Q9H964
Accessions EAW89661 EAW89662 EAW89663 Q8TAT6 AB040932 BAA96023 AK000664 BAA91314 AK023046 BAB14372 AK023272 BAB14499 AK024398 AK074322 AK294473 BAG57700 AK311741 AL133629 AL834300 CAD38971 BC013406 BC025930 AAH25930 BU542911 CK903591 DA998574 DC322260 DQ587272 DQ587568 DQ895356 ABM86282 HY116973 HY179409 XM_011524979 XP_011523281 XM_011524980 XP_011523282 XM_011524981 XP_011523283 XM_011524982 XP_011523284 XR_934501 XR_934502 NM_017921 NP_060391 NR_130139 NR_130140
Function The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}. Endoplasmic reticulum {ECO:0000250}. Nucleus {ECO:0000250}. Note=Associated with the endoplasmic reticulum and nuclear. {ECO:0000250}.
Tissue Specificity Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver. {ECO:0000269|PubMed:11574150}.
Top Pathways Protein processing in endoplasmic reticulum

NPL4 Antibody - 13489 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using NPL4 Antibody.

Npl4 (D-1) - sc-365796 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Npl4 (D-1): sc-365796. Western blot analysis of Npl4 expression in SK-BR-3 whole cell lysate.

Npl4 (H-300) - sc-134746 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Npl4 (H-300): sc-134746. Western blot analysis of Npl4 expression in SK-N-MC (A) and SH-SY5Y (B) whole cell lysates.

Npl4 (K-20) - sc-49463 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Npl4 (N-20) - sc-49464 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Npl4 (N-20): sc-49464. Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.

Anti-NPL4 antibody - ab101226 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NPL4 antibody (ab101226) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 4 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NPL4 antibody - ab172808 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NPL4 antibody (ab172808) at 1 µg/mlLane 1 : NPL4 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Rabbit IgG (H+L), peroxidase conjugate at 1/7500 dilutiondeveloped using the ECL technique

Anti-NPL4 antibody - ab68020 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-NPL4 antibody (ab68020) at 1/500 dilution + human spleen tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-NPL4 antibody - ab176383 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of NPL4 in immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176383 at 6 µg/mg lysate for IP. Lane 2 represents control IgG IP. An anti-NPL4 antibody was used at 0.1 µg/ml for subsequent western blot detection.Detection: Chemiluminescence with exposure time of 3 minutes.

Anti-NPL4 antibody - C-terminal - ab173087 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NPL4 antibody - C-terminal (ab173087) at 1/100 dilution + CEM cell line lysate at 35 µg

Anti-NPL4 antibody - N-terminal - ab177445 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NPL4 antibody - N-terminal (ab177445) at 1 µg/ml + Mouse testis lysate at 10 µg

Anti-NPLOC4 Antibody (aa456-484, APC) - LS-C264618 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa456-484, Biotin) - LS-C264619 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa456-484, AP) - LS-C264617 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa456-484, Azide-free, HRP) - LS-C264621 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa456-484, FITC) - LS-C264620 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa525-575) - LS-C290748 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa456-484, PE) - LS-C264622 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa455-484) - LS-C323414 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa96-145) - LS-C170369 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-NPLOC4 Antibody (aa75-125) - LS-C290747 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse NPL4 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NPL4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-NPLOC4 Antibody (aa456-484) - LS-C158418 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NPLOC4 Antibody western blot of CEM cell line lysates (35 ug/lane). The NPLOC4 antibody detected the NPLOC4 protein (arrow).

NPLOC4 Antibody - NBP1-82166 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NPLOC4 Antibody [NBP1-82166] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).

NPLOC4 Antibody - NBP1-82165 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NPLOC4 Antibody - H00055666-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
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