RAPGEF4

Gene Symbol RAPGEF4
Entrez Gene 11069
Alt Symbol 2, CAMP-GEFII, CGEF2, EPAC, EPAC 2, EPAC2, Nbla00496
Species Human
Gene Type protein-coding
Description Rap guanine nucleotide exchange factor (GEF) 4
Other Description RAP guanine-nucleotide-exchange factor (GEF) 4|cAMP-regulated guanine nucleotide exchange factor II|exchange factor directly activated by cAMP 2|exchange protein directly activated by cAMP 2|putative protein product of Nbla00496|rap guanine nucleotide exchange factor 4
Swissprots Q8WZA2 B7Z912 Q8IXK6 B7Z3T6 O95636 B7Z283 B2R7R3 Q8TAA4
Accessions AAY15071 AAY24186 CDM22142 EAX11167 EAX11168 EAX11169 Q8WZA2 AB027471 BAB72179 AB075513 BAE45759 AB209681 BAD92918 AK294433 BAH11764 AK294445 BAH11769 AK294914 BAH11923 AK294997 BAH11944 AK295002 BAH11946 AK296340 BAH12322 AK302732 BAH13791 AK304278 BAH14148 AK310373 AK311165 AK311500 AK311575 AK311697 AK313084 BAG35910 AK316014 BAH14385 AK316072 BAH14443 BC024004 AAH24004 BC040183 AAH40183 BI546558 BQ184791 DB471672 DC335305 DQ890549 ABM92166 DQ894011 ABM84937 KJ534924 AHW56564 KJ534925 AHW56565 KJ534978 AHW56618 U78516 AAD03422 XM_005246246 XP_005246303 XM_006712205 XP_006712268 XM_011510505 XP_011508807 NM_001100397 NP_001093867 NM_001282899 NP_001269828 NM_001282900 NP_001269829 NM_001282901 NP_001269830 NM_007023 NP_008954
Function Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA- independent exocytosis through interaction with RIMS2 (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
Tissue Specificity Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.
Top Pathways Leukocyte transendothelial migration, Adrenergic signaling in cardiomyocytes, cAMP signaling pathway, Rap1 signaling pathway

GABP-β1/2 (E-1) - sc-271531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GABP-β1/2 (E-1): sc-271531. Western blot analysis of GABP-β1/2 expression in Jurkat whole cell lysate.

GABP-β1/2 (E-7) - sc-271571 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GABP-β1/2 (E-7): sc-271571. Western blot analysis of GABP-β1/2 expression in Jurkat whole cell lysate (A) and HeLa nuclear extract (B).

TFIIIB90-1/2/3/5 (H-40) - sc-98583 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TFIIIB90-1/2/3/5 (H-40): sc-98583. Western blot analysis of TFIIIB90-1 expression in non-transfected: sc-117752 (A) and human TFIIIB90-1 transfected: sc-111635 (B) 293T whole cell lysates and Hep G2 nuclear extract (C).

p-Akt1/2/3 (Ser 473)-R - sc-7985-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of Akt1 phosphorylation in A-431 cells treated with EGF. Blots were probed with Akt1 (C-20): sc-1618 (A), p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate unphosphorylated peptide (B) and p-Akt1/2/3 (Ser 473): sc-7985-R preincubated with cognate phosphorylated peptide (C).

p-Akt1/2/3 (Thr 308)-R - sc-16646-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
p-Akt1/2/3 (Thr 308)-R: sc-16646-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing nuclear localization.

p-Rsk-1/2/4 (Ser 363)-R - sc-17033-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of Rsk-1/2/4 phosphorylation in HeLa whole cell lysate (A-C). Blots were probed with p-Rsk-1/2/4 (Ser 363)-R: sc-17033-R preincubated with cognate phosphorylated (A) and unphosphorylated (B) peptide and Rsk-1 (C-21): sc-231 (C).

p-4E-BP1/2/3 (Thr 45)-R - sc-23767-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal

p-4E-BP1/2/3 (Thr 45)-R - sc-18090-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected 293T: sc-117752 (A) and human 4E-BP1 transfected 293T: sc-116590 (B) whole cell lysates and HeLa nuclear extract (C).

Epac2 (A-7) - sc-28326 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Epac2 (A-7): sc-28326. Western blot analysis of Epac2 expression in rat brain tissue extract.

Epac2 (C-6) - sc-390690 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Epac2 (C-6): sc-390690. Western blot analysis of Epac2 expression in human skeletal muscle tissue extract.

Epac2 (H-220) - sc-25633 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Epac2 (H-220): sc-25633. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing membrane localization.

Epac2 (N-15) - sc-26364 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Epac2 (M-18) - sc-9383 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Epac2 (M-18): sc-9383. Western blot analysis of Epac2 expression in rat cerebellum (A) and rat brain (B) tissue extract.

Anti-Epac2 antibody - ab21238 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab21238 staining Epac2 in the bronchial surface epithelial 16HBE14o- cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were rinsed twice with phosphate-buffered saline (PBS) and then fixed with 4% paraformaldehyde for 30 minutes at 4°C, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes and blocked with 10% fetal calf serum (FCS) in PBS for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 10% FCS) overnight at 4°C. An Alex Fluor®568-conjugated Goat anti-rabbit IgG was used as the secondary antibody. The corresponding image taken in brightfield is shown on the right.

Anti-Epac2 antibody - ab124189 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of Epac2 in mouse liver tissue with ab124189 at 20 ug/mL.

Anti-Epac2 antibody - ab172679 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Epac2 antibody (ab172679) at 1 µg/mlLane 1 : Epac2 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.SecondaryLane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutionLane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 2500 developed using the ECL technique

Anti-Epac2 antibody - ab56863 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56863 Epac2 antibody at 1ug/ml.

Anti-Epac2 antibody [EPR15246] - ab193665 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15246
All lanes : Anti-Epac2 antibody [EPR15246] (ab193665) at 1/5000 dilutionLane 1 : Rat brain lysateLane 2 : Mouse brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Epac2 antibody - N-terminal - ab189974 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Epac2 antibody - N-terminal (ab189974) at 1 µg/mlLane 1 : Rat liver tissue lysateLane 2 : Rat liver tissue lysate with immunizing peptide

Anti-Bone Proteoglycan 1/2 (BGN/DCN Antibody (aa4-17) - LS-C27307 from LifeSpan Bioscience

Prices $420.00
Sizes 1 ml
Host Sheep
Clonality Polyclonal

Anti-Cytochrome P450 CYP1A1/2 Antibody (aa49-98) - LS-C291852 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Cytochrome P450 CYP1A1/2 Antibody (Arg98) - LS-C176851 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Western blot of CYP1A1/2 (R98) pAb in extracts from RAW264.7 cells.

Anti-Cytochrome P450 CYP1A1/2 Antibody (aa71-120) - LS-C119486 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Western blot of extracts from COS7/RAW264.7/HT-29 cells, using Cytochrome P450 1A1/2 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-Gi Alpha 1/2 Antibody (aa345-354) IHC-plus™ - LS-B750 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Gi Alpha 1/2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B750 concentration 10 ug/ml.

Anti-RAPGEF4 / EPAC2 Antibody (N-Terminus) IHC-plus™ - LS-B9590 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RAPGEF4 / EPAC2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9590 concentration 5 ug/ml.
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