EOGT

Gene Symbol EOGT
Entrez Gene 285203
Alt Symbol AER61, AOS4, C3orf64, EOGT1
Species Human
Gene Type protein-coding
Description EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase
Other Description AER61 glycosyltransferase|EGF domain-specific O-linked N-acetylglucosamine transferase|EGF-O-GlcNAc transferase|extracellular O-linked N-acetylglucosamine transferase
Swissprots Q6ZTV0 Q6P985 L7X1M5 B4DFH5 A8K2U1 Q5NDL2 Q6MZY0
Accessions Q5NDL2 AA709285 AJ868234 CAI30569 AK023140 AK091089 BAG52283 AK123213 AK126187 BAC86479 AK290356 BAF83045 AK294101 BAG57436 AK304102 BAG65005 AL833467 BC028935 AAH28935 BC060887 AAH60887 BX640821 CAE45897 BX648818 DA856791 HQ448550 ADQ33029 KC347596 AGC92970 XM_005264743 XP_005264800 XM_011533599 XP_011531901 XM_011533600 XP_011531902 XM_011533601 XP_011531903 XM_011533602 XP_011531904 XM_011533603 XP_011531905 XM_011533604 XP_011531906 XM_011533605 XP_011531907 NM_001278689 NP_001265618 NM_173654 NP_775925 NR_103826
Function Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N- acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains. {ECO:0000269|PubMed:23671640}.
Subcellular Location Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138}.
Top Pathways Other types of O-glycan biosynthesis

EOGT (S-13) - sc-99824 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
C3orf64 (S-13): sc-99824. Western blot analysis of C3orf64 expression in SK-N-SH (A) and IMR-32 (B) whole cell lysates.

EOGT (N-14) - sc-99823 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-AER61 antibody - ab69389 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-AER61 antibody (ab69389) at 1/500 dilutionLane 1 : AER61 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-AER61 antibody [EPR12944] - ab190693 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12944
All lanes : Anti-AER61 antibody [EPR12944] (ab190693) at 1/1000 dilutionLane 1 : Neuro-2a cell lysateLane 2 : U87-MG cell lysateLane 3 : HeLa cell lysateLane 4 : Caco 2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-EOGT Antibody - LS-C306125 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-EOGT Antibody (C-Terminus) - LS-C82562 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

EOGT/AER61 Antibody - NBP1-90224 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EOGT/AER61 Antibody [NBP1-90224] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp

EOGT/AER61 Antibody - NBP1-57931 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EOGT/AER61 Antibody [NBP1-57931] -  Human Brain lysate, concentration 0.2-1 ug/ml.

EOGT/AER61 Antibody - H00285203-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

AER61 Polyclonal Antibody - bs-9096R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes;  Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-AER61 Polyclonal Antibody, Unconjugated (bs-9096R) at 1:500 overnight at 4°C, followed by a conjugated secondary  for 90 minutes and DAB staining

Anti-C3ORF64 antibody produced in rabbit - AV49111 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: fetal brain (<B>Cat. No. AV49111</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1 μg/mL in cell type fetal brain

Anti-EOGT antibody produced in rabbit - HPA019460 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-C3orf64: <B>Cat. No. HPA019460</B>: Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Anti-AER61 antibody produced in mouse - SAB1408482 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-AER61: <B>Cat. No. SAB1408482</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~48.7 kDa using C3orf64 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

C3orf64 antibody - C-terminal region (ARP49111_P050) - ARP49111_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

C3ORF64 antibody, C-term - GTX46130 from GeneTex

Prices $319.00
Sizes 50 μg
Host Rabbit
Clonality Polyclonal
Anti-C3ORF64 antibody, C-term used in Western blot. GTX46130

C3ORF64 antibody - 70R-5375 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

EOGT / AER61 (Isoform 1+3) antibody - AP43600PU-N from Acris Antibodies

Prices $340.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Brain; WB Suggested Anti-C3orf64 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; C3orf64 antibody - C-terminal region (AP43600PU-N) in Human Brain cells using Western Blot

EOGT / AER61 antibody - H00285203-B01P from Acris Antibodies

Prices $360.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot analysis of C3orf64 expression in transfected 293T cell line (H00285203-T02) by C3orf64 MaxPab polyclonal antibody.  Lane 1: AER61 transfected lysate(48.73 KDa). Lane 2: Non-transfected lysate.

C3orf64 Antibody - 30-506 from ProSci

Prices $315.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Antibody used in WB on Human Brain at 0.2-1 ug/ml.

C3orf64 Antibody - GWB-MR404H from Genway Biotech

Prices $368.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

AER61, Polyclonal Antibody - MBS645199 from MyBioSource

Prices $580.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-C3ORF64 monoclonal antibody - DCABH-14827 from Creative Diagnostics

Host Rabbit
Clonality Monoclonal

AER61 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-9096R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

AER61 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-9096R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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