| Gene Symbol | ACER2 |
|---|---|
| Entrez Gene | 340485 |
| Alt Symbol | ALKCDase2, ASAH3L |
| Species | Human |
| Gene Type | protein-coding |
| Description | alkaline ceramidase 2 |
| Other Description | alkCDase 2|alkaline CDase 2|ceramide hydrolase|haCER2 |
| Swissprots | A2A3R8 Q5VZR7 Q569G5 Q5QJU3 Q71RD2 |
| Accessions | EAW58634 Q5QJU3 AF370405 AAQ15241 AK123581 BAG53917 AY312516 AAQ85132 BC092487 AAH92487 XM_005251447 XP_005251504 XM_005251448 XP_005251505 XM_011517856 XP_011516158 XM_011517857 XP_011516159 XM_011517858 XP_011516160 XM_011517859 XP_011516161 XR_242505 XR_242506 XR_242507 XR_929240 XR_929241 NM_001010887 NP_001010887 |
| Function | Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)- ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)- ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate. {ECO:0000269|PubMed:16940153, E |
| Subcellular Location | Golgi apparatus membrane {ECO:0000269|PubMed:16940153, ECO:0000269|PubMed:20089856}; Multi- pass membrane protein {ECO:0000269|PubMed:16940153, ECO:0000269|PubMed:20089856}. |
| Tissue Specificity | Highly expressed in placenta. {ECO:0000269|PubMed:16940153}. |
| Top Pathways | Sphingolipid signaling pathway, Sphingolipid metabolism |
ASAH3L CRISPR/Cas9 KO Plasmid (h) - sc-415541 from Santa Cruz Biotechnology
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ASAH3L HDR Plasmid (h) - sc-415541-HDR from Santa Cruz Biotechnology
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ASAH3L Double Nickase Plasmid (h) - sc-415541-NIC from Santa Cruz Biotechnology
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ASAH3L Double Nickase Plasmid (h2) - sc-415541-NIC-2 from Santa Cruz Biotechnology
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ASAH3L CRISPR Activation Plasmid (h) - sc-415541-ACT from Santa Cruz Biotechnology
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ASAH3L CRISPR Activation Plasmid (h2) - sc-415541-ACT-2 from Santa Cruz Biotechnology
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ASAH3L Lentiviral Activation Particles (h) - sc-415541-LAC from Santa Cruz Biotechnology
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ASAH3L Lentiviral Activation Particles (h2) - sc-415541-LAC-2 from Santa Cruz Biotechnology
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ASAH3L siRNA (h) - sc-92953 from Santa Cruz Biotechnology
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ASAH3L shRNA Plasmid (h) - sc-92953-SH from Santa Cruz Biotechnology
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ASAH3L shRNA (h) Lentiviral Particles - sc-92953-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU113321 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2716 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2716 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR10433 from Sigma-Aldrich
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ACER2 siRNA (Human) - CRJ7348 from Cohesion Biosciences
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ACER2 - MBS8229945 from MyBioSource
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shRNA set against Human ACER2 (NM_001010887.2) - SHH230242 from Creative biogene
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shRNA set against Human ASAH3L(NM_001010887.2) - SHG029341 from Creative biogene
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Human ACER2 siRNA - orb277849 from Biorbyt
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ACER2 shRNA Plasmid (Human) - CHJ7348 from Cohesion Biosciences
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ACER2 miRNA 3'UTR clone - MiUTR1H-00618 from Creative biogene
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