TRIT1

Gene Symbol	TRIT1
Entrez Gene	54802
Alt Symbol	IPT, MOD5
Species	Human
Gene Type	protein-coding
Description	tRNA isopentenyltransferase 1
Other Description	IPP transferase\|IPPT\|IPTase\|hGRO1\|isopentenyl-diphosphate:tRNA isopentenyltransferase\|tRNA dimethylallyltransferase, mitochondrial\|tRNA isopentenylpyrophosphate transferase
Swissprots	Q3T7B5 Q9NXT7 Q5QPK5 Q96L45 Q96FJ3 Q6IAC9 Q9H3H1 Q5QPK6 A1A4X7
Accessions	EAX07252 EAX07253 Q9H3H1 AF074918 AAG31324 AK000068 BAA90923 AK074222 AK223478 BAD97198 AK296453 BAG59101 AK304759 BAG65514 AK307910 AY052768 AAL14107 AY303390 AAP60111 AY702933 AAW63394 AY702934 AAW63395 AY702935 AAW63396 AY702936 AAW63397 AY702937 AAW63398 AY702938 AAW63399 AY702939 AAW63400 AY702940 AAW63401 AY702941 AAW63402 AY702942 AAW63403 AY702943 AAW63404 AY702944 AAW63405 AY702945 AAW63406 AY702946 AAW63407 AY702947 BC010741 AAH10741 BC107569 AAI07570 BC128155 AAI28156 BC128156 AAI28157 CR457226 CAG33507 DA031689 XM_005270954 XP_005271011 XM_006710706 XP_006710769 XR_246278 XR_946671 XR_946672 NM_017646 NP_060116
Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). {ECO:0000269\|PubMed:11111046}.
Subcellular Location	Isoform 5: Cytoplasm {ECO:0000305}.

MOD5 (I-20) - sc-54535 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

MOD5 (Y-19) - sc-54536 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-TRIT1 antibody - ab87166 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TRIT1 antibody (ab87166) at 1 µg/ml + Human fetal heart lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-TRIT1 antibody - ab71843 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-TRIT1 antibody (ab71843) at 1/500 dilutionLane 1 : TRIT1 transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-TRIT1 antibody [EPR12517] - ab174308 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR12517

Flow cytometric analysis of permeabilized Raji cells labeling TRIT1 with ab174308 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Anti-TRIT1 Antibody (C-Terminus) - LS-C30387 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TRIT1 Antibody (aa20-69) - LS-C145481 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

TRIT1 antibody LS-C145481 Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml.

Anti-TRIT1 Antibody (aa179-228) - LS-C101417 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

TRIT1 antibody LS-C101417 Western blot of Fetal Heart lysate.

TRIT1 Antibody - NBP1-82210 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: TRIT1 Antibody [NBP1-82210] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4

TRIT1 Antibody - NBP1-80013 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: TRIT1 Antibody [NBP1-80013] - Titration: 0.2-1 ug/ml, Positive Control: Human heart.

TRIT1 Antibody - NBP2-20727 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: TRIT1 Antibody [NBP2-20727] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

TRIT1 Antibody - H00054802-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: TRIT1 Antibody [H00054802-B01] - analysis of TRIT1 expression in transfected 293T cell line by TRIT1 MaxPab polyclonal antibody (B01). Lane 1: TRIT1 transfected lysate (52.70 KDa). Lane 2: Non-transfected lysate.

TRIT1 Antibody - H00054802-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-TRIT1 antibody produced in mouse - SAB1409466 from Sigma-Aldrich

Prices	$347.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-TRIT1: <B>Cat. No. SAB1409466</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~51.4 kDa using TRIT1 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-TRIT1 antibody produced in rabbit - SAB2103155 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-TRIT1, antibody produced in rabbit: <B>Cat. No. SAB2103155</B>: Immunoblotting analysis of TRIT1 in fetal heart tissue lysate with TRIT1 anitbody at 1 μg/mL.

Anti-TRIT1 antibody produced in rabbit - AV39298 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: Jurkat (<B>Cat. No. AV39298</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.25 μg/mL in cell type Jurkat

Anti-TRIT1 antibody produced in rabbit - HPA024174 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-TRIT1: <B>Cat. No. HPA024174</B>: Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

TRIT1 antibody - middle region (ARP32883_P050) - ARP32883_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

TRIT1 antibody - C-terminal region (ARP39298_P050) - ARP39298_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

TRIT1 antibody - 70R-8332 from Fitzgerald

Prices	$345.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

TRIT1 antibody - 70R-7956 from Fitzgerald

Prices	$345.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

TRIT1 antibody - H00054802-B01 from Acris Antibodies

Prices	$360.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot analysis of TRIT1 expression in transfected 293T cell line (H00054802-T01) by TRIT1 MaxPab polyclonal antibody.Lane 1: TRIT1 tra

TRIT1 antibody - H00054802-B01P from Acris Antibodies

Prices	$360.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

TRIT1 Antibody - 25-100 from ProSci

Prices	$315.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Antibody used in WB on Human Heart at 0.2-1 ug/ml.

TRIT1 Antibody - 29-040 from ProSci

Prices	$315.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

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