MED19

Gene Symbol MED19
Entrez Gene 219541
Alt Symbol DT2P1G7, LCMR1
Species Human
Gene Type protein-coding
Description mediator complex subunit 19
Other Description lung cancer metastasis-related protein 1|mediator of RNA polymerase II transcription subunit 19|mediator of RNA polymerase II transcription, subunit 19 homolog
Swissprots Q8IV02 A0JLT2 Q8IZD1
Accessions A0JLT2 EAW73774 EAW73775 EAW73776 AY148462 AAN16075 BC009723 AAH09723 BC037223 AAH37223 HE646678 CCF17579 U92995 XM_005273821 XP_005273878 NM_153450 NP_703151
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Subcellular Location Nucleus {ECO:0000305}.

Med19 (S-19) - sc-161839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Med19 (D-12) - sc-161837 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Med19 (T-20) - sc-161840 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MED19 antibody - ab49271 from Abcam

Prices $242.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab49271 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49271, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4% PFA at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

Anti-MED19 Antibody (aa161-188, APC) - LS-C237515 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED19 Antibody (aa161-188, Biotin) - LS-C237516 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED19 Antibody (aa161-188, AP) - LS-C237513 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED19 Antibody (aa161-188, PE) - LS-C237518 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED19 Antibody (aa161-188, Azide-free, HRP) - LS-C248790 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED19 Antibody (aa161-188, FITC) - LS-C237517 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED19 Antibody (aa160-188) - LS-C322936 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MED19 Antibody (aa68-117) - LS-C135344 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MED19 antibody LS-C135344 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml.

Anti-MED19 Antibody (aa118-167) - LS-C201658 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human Jurkat. MED19 antibody dilution 1.0 ug/ml.

Anti-MED19 Antibody (aa161-188) - LS-C158123 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MED19 Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The MED19 antibody detected the MED19 protein (arrow).

MED19 Antibody - NBP1-81320 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MED19 Antibody - NBP1-81319 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MED19 Antibody [NBP1-81319] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

MED19 Antibody - NBP1-79597 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MED19 Antibody [NBP1-79597] - Rat Liver Lysate, probed with MED19 Antibody, diluted at 1 ug/ml.

Anti-MED19 (176-190) antibody produced in rabbit - SAB1103492 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human MED19 was separated on SDS-PAGE, blotted with Anti-MED19 (176-190) (<B>Cat. No. SAB1103492</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500

Anti-MED19 antibody produced in rabbit - HPA040860 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED19: <B>Cat. No. HPA040860</B>: Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.

Anti-MED19 antibody produced in rabbit - HPA039912 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED19: <B>Cat. No. HPA039912</B>: Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.

MED19 Antibody - middle region (ARP55596_P050) - ARP55596_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MED19-Antibody-C-term - AP18645b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
MED19 Antibody (C-term) (Cat. #AP18645b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MED19 antibody detected the MED19 protein (arrow).

MED19 Antibody - C-terminal region (OAAB14184) - OAAB14184 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
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