TPRKB

Gene Symbol TPRKB
Entrez Gene 51002
Alt Symbol CGI-121
Species Human
Gene Type protein-coding
Description TP53RK binding protein
Other Description EKC/KEOPS complex subunit TPRKB|PRPK (p53-related protein kinase)-binding protein|PRPK-binding protein|TP53RK-binding protein
Swissprots Q8IWR7 Q8IWR6 D6W5H6 Q9Y3C4 Q9H3K4
Accessions CAJ42830 EAW99720 EAW99721 EAW99722 EAW99723 Q9Y3C4 AF060921 AAG43133 AF151879 AAD34116 AI759966 AK097254 AK291071 BAF83760 AK295239 BAG58232 AY157986 AAN76356 AY157987 AAN76357 AY643713 AAT64915 BC029492 AAH29492 DA688967 XM_005264343 XP_005264400 XM_005264345 XP_005264402 XM_006712026 XP_006712089 XM_006712027 XP_006712090 XM_011532877 XP_011531179 XM_011532878 XP_011531180 XM_011532879 XP_011531181 XM_011532880 XP_011531182 NM_016058 NP_057142
Function Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm. Nucleus.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:12659830}.

CGI-121 (N-14) - sc-242431 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-TPRKB antibody - ab96261 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-TPRKB antibody (ab96261) at 1/500 dilution + 293T whole cell lysate at 30 µg

Anti-TPRKB antibody - ab68245 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-TPRKB antibody (ab68245) at 1/500 dilutionLane 1 : TPRKB transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-TPRKB antibody [3H3] - ab128112 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H3
All lanes : Anti-TPRKB antibody [3H3] (ab128112) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.

Anti-TPRKB Antibody - LS-C125351 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-TPRKB Antibody (clone 2165C3a) - LS-C184720 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2165C3a

Anti-TPRKB Antibody (aa66-176) - LS-C306920 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-TPRKB Antibody (aa35-84) - LS-C107977 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TPRKB antibody LS-C107977 Western blot of THP-1 cell lysate.

Anti-TPRKB Antibody (clone 2165C3a) - LS-C342748 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2165C3a
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CGI-121 mAb.

Anti-TPRKB Antibody (clone 1E9) - LS-C336942 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E9
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Anti-TPRKB Antibody (aa1-175) - LS-C185835 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Ca922 xenograft using TPRKB antibody at 1:250 dilution.

Anti-TPRKB Antibody (clone 3E3) - LS-C175151 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E3
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody.

Anti-TPRKB Antibody (clone 3H3) - LS-C175160 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H3
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody.

TPRKB Antibody (3H3) - NBP2-03885 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H3
Western Blot: TPRKB Antibody (3H3) [NBP2-03885] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-TPRKB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

TPRKB Antibody - NBP1-31618 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TPRKB Antibody [NBP1-31618] - Sample(30 ug whole cell lysate)A:293T 12% SDS PAGE, antibody diluted at 1:500.

TPRKB Antibody - NBP1-56860 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TPRKB Antibody [NBP1-56860] - Titration: 0.2-1 ug/ml, Positive Control: THP-1 cell lysate.

TPRKB Antibody - H00051002-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: TPRKB Antibody [H00051002-D01P] - Analysis of TPRKB expression in transfected 293T cell line by TPRKB polyclonal antibody.Lane 1: TPRKB transfected lysate(19.70 KDa).Lane 2: Non-transfected lysate.

TPRKB Antibody - H00051002-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

TPRKB Antibody - H00051002-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Monoclonal Anti-CGI-121 antibody produced in mouse - WH0051002M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F6

Anti-TPRKB antibody produced in rabbit - SAB1410891 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-TPRKB: <B>Cat. No. SAB1410891</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~19.7 kDa using TPRKB transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-TPRKB antibody produced in rabbit - HPA035712 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-TPRKB: <B>Cat. No. HPA035712</B>: Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity often combined with nuclear and membranous staining in tubular cells.

Anti-TPRKB antibody produced in mouse - SAB1407428 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-TPRKB: <B>Cat. No. SAB1407428</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~19.3 kDa using TPRKB transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-TPRKB antibody produced in rabbit - SAB2105179 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-TPRKB, antibody produced in rabbit: <B>Cat. No. SAB2105179</B>: Immunoblotting analysis of TPRKB in THP-1 cell lysate with TPRKB anitbody at 1.0μg/mL.

TPRKB antibody - middle region (ARP56819_P050) - ARP56819_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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