TTF2

Gene Symbol	TTF2
Entrez Gene	8458
Alt Symbol	HuF2, ZGRF6
Species	Human
Gene Type	protein-coding
Description	transcription termination factor, RNA polymerase II
Other Description	F2\|RNA polymerase II termination factor\|human factor 2\|lodestar homolog\|lodestar protein\|transcription release factor 2\|transcription termination factor 2\|zinc finger, GRF-type containing 6
Swissprots	Q9UNY4 O75921 Q8N6I8 Q5VVU8 Q5T2K7 A8K4Q2
Accessions	EAW56666 EAW56667 Q9UNY4 AB209845 BAD93082 AF073771 AAC64044 AF080255 AAD49435 AI991355 AK291017 BAF83706 AW027795 BC015341 BC030058 AAH30058 BI857056 BQ422564 BU182600 H81916 XM_005271277 XP_005271334 XM_006710979 XP_006711042 XM_006710980 XP_006711043 XM_006710981 XP_006711044 XM_011542303 XP_011540605 XM_011542304 XP_011540606 XM_011542305 XP_011540607 XR_946782 NM_003594 NP_003585
Function	DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing. {ECO:0000269\|PubMed:10455150, ECO:0000269\|PubMed:12927788, ECO:0000269\|PubMed:15125840, ECO:0000269\|PubMed:9748214}.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:15125840}. Nucleus {ECO:0000269\|PubMed:15125840}. Note=Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis.
Top Pathways	Thyroid hormone synthesis

TTF2 (C-19) - sc-54429 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

TTF2 (S-19) - sc-54433 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-TTF2 antibody - ab155177 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TTF2 antibody (ab155177) at 1/5000 dilution + H1299 whole cell lysate at 30 µg

Anti-TTF2 antibody - ab28030 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Sheep
Clonality	Polyclonal

Anti-TTF2 antibody (ab28030) at 1/1000 dilution + HeLa nuclear extractSecondaryrabbit anti-sheep peroxidase linked at 1/10000 dilution

Anti-TTF2 antibody - ab112078 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-TTF2 antibody (ab112078) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-TTF2 antibody - ab56451 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-TTF2 antibody [EPR12756] - ab181116 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR12756

All lanes : Anti-TTF2 antibody [EPR12756] (ab181116) at 1/5000 dilutionLane 1 : Daudi cell line lysateLane 2 : HeLa cell line lysateLane 3 : Jurkat cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Anti-TTF2 antibody - N-terminal - ab175605 from Abcam

Host	Rabbit
Clonality	Polyclonal

Anti-TTF2 antibody - N-terminal (ab175605) at 1/1000 dilution + HL60 cell line lysates at 35 µg

Anti-TTF2 / HuF2 Antibody - LS-C332032 from LifeSpan Bioscience

Prices	$305.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TTF2 / HuF2 Antibody (aa225-275) - LS-C289807 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human TTF2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TTF2 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. TTF2 was also immunoprecipitated by rabbit anti-TTF2 antibody BL11089, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Anti-TTF2 / HuF2 Antibody (clone 3D11) - LS-C198567 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3D11

TTF2 monoclonal antibody (M02), clone 3D11 Western Blot analysis of TTF2 expression in PC-12.

TTF2 Antibody (1E8) - H00008458-M06 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1E8

Western Blot: TTF2 Antibody (1E8) [H00008458-M06] - TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in human placenta.

TTF2 Antibody (3D11) - H00008458-M02 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3D11

Western Blot: TTF2 Antibody (3D11) [H00008458-M02] - TTF2 monoclonal antibody (M02), clone 3D11 Analysis of TTF2 expression in PC-12.

TTF2 Antibody (2B6) - H00008458-M12 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2B6

TTF2 Antibody (3F7) - H00008458-M09 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3F7

Western Blot: TTF2 Antibody (3F7) [H00008458-M09] - Analysis of TTF2 expression in MES-SA/Dx5 (Cat # L021V1).

TTF2 Antibody (1E4.) - H00008458-M10A from Novus Biologicals

Prices	$359.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Clone	1E4.

Western Blot: TTF2 Antibody (1E4) [H00008458-M10A] - Analysis of TTF2 expression in U-2 OS (Cat # L022V1).

TTF2 Antibody - H00008458-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Monoclonal Anti-TTF2 antibody produced in mouse - SAB1409897 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2B6

<B>Immunoblotting</B><BR/>Monoclonal Anti-TTF2: <B>Cat. No. SAB1409897</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~129.6 kDa using MES-SA/Dx5 lysate.

Monoclonal Anti-TTF2 antibody produced in mouse - SAB1409896 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3F7

<B>Immunoblotting</B><BR/>Monoclonal Anti-TTF2: <B>Cat. No. SAB1409896</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~129.6 kDa using MES-SA/Dx5 lysate.

Anti-TTF2 antibody produced in rabbit - HPA005776 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-TTF2: <B>Cat. No. HPA005776</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing membranous and/or cytoplasmic staining of cells in ductus seminiferus.

Monoclonal Anti-TTF2 antibody produced in mouse - WH0008458M2 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3D11

<B>Immunoblotting</B><BR/>Monoclonal Anti-TTF2, <B>Cat. No. WH0008458M2</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~125 kDa using PC-12 lysate.

Monoclonal Anti-TTF2 antibody produced in mouse - SAB1404582 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1E8

<B>Immunofluorescence</B><BR/>Monoclonal Anti-TTF2: <B>Cat. No. SAB1404582</B>: Antibody concentration: 10 μg/mL using HeLa cell.

TTF2 Antibody - N-terminal region (ARP75197_P050) - ARP75197_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

TTF2-Antibody-monoclonal-M12 - AT4390a from Abgent, a WuXi AppTec company

Prices	$315.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2B6

Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (39.75 KDa) .

TTF2-Antibody-monoclonal-M09 - AT4389a from Abgent, a WuXi AppTec company

Prices	$315.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3F7

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