BRSK1

Gene Symbol BRSK1
Entrez Gene 84446
Alt Symbol hSAD1
Species Human
Gene Type protein-coding
Description BR serine/threonine kinase 1
Other Description BR serine/threonine-protein kinase 1|SAD1 homolog|SAD1 kinase|SadB kinase short isoform|brain-selective kinase 1|brain-specific serine/threonine-protein kinase 1|protein kinase SAD1A|serine/threonine-protein kinase BRSK1|serine/threonine-protein kinase SAD-B|synapses of Amphids Defective homolog 1
Swissprots Q96JL4 Q5J5B5 F1DG44 Q96AV4 Q8TDC3 Q8TDC2 Q8NDR4 Q8NDD0
Accessions EAW72359 EAW72360 EAW72361 Q8TDC3 AB058714 BAB47440 AF479826 AAL87697 AF479827 AAL87698 AK124999 BAG54120 AL831945 CAD38595 AL834275 CAD38950 AY458602 AAS10354 AY505124 AAS86442 BC016681 AAH16681 HQ830199 ADX95745 XM_005259327 XP_005259384 XM_011527395 XP_011525697 XR_430213 NM_032430 NP_115806
Function Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma- tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, pro
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15150265}. Nucleus {ECO:0000269|PubMed:15150265}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Cell junction, synapse {ECO:0000250}. Note=Localizes to synaptic vesicles in neurons (By similarity). Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS- induced DNA damage. {ECO:0000250}.
Tissue Specificity Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle. {ECO:0000269|PubMed:15150265}.

BRSK1 (D10F2) Rabbit mAb - 5935 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10F2
Western blot analysis of extracts from rat and fetal rat brain using BRSK1 (D10F2) Rabbit mAb.

BRSK1 (B-1) - sc-376099 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BRSK1 (B-1): sc-376099. Western blot analysis of BRSK1 expression in H4 whole cell lysate.

BRSK1 (E-13) - sc-167221 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BRSK1 (E-13): sc-167221. Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of neuronal cells and glial cells.

BRSK1 (G-13) - sc-167222 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

BRSK1 (H-110) - sc-134644 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BRSK1 (H-110): sc-134644. Western blot analysis of BRSK1 expression in mouse brain tissue extract.

BRSK1 (N-22) - sc-130122 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
BRSK1 (N-22): sc-130122. Western blot analysis of BRSK1 expression in Hep G2 (A), Jurkat (B), K-562 (C) and Raji (D) whole cell lysates and human liver (E) and mouse liver (F) tissue extracts.

Anti-BRSK1 antibody - ab140436 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of BRSK1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140436 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-BRSK1 antibody which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-BRSK1 antibody - ab115475 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab115475, at 5µg/ml, staining BRSK1 in formalin-fixed, paraffin-embedded Human brain cortex tissue by immunohistochemistry using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-BRSK1 antibody - ab117251 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab117251, at 5 µg/ml, staining BRSK1 in Formalin-fixed, Paraffin-embedded Human brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-BRSK1 antibody - ab135993 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BRSK1 antibody (ab135993) at 1/500 dilution + lysates from COLO205 cells at 30 µg

Anti-BRSK1 antibody - N-terminal - ab71528 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BRSK1 antibody - N-terminal (ab71528) at 1/60 dilution + mouse liver tissue lysate at 35 µg

Anti-KIAA1811 / BRSK1 Antibody (C-Terminus) IHC-plus™ - LS-B180 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BRSK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B180 concentration 5 ug/ml.

Anti-KIAA1811 / BRSK1 Antibody (C-Terminus) - LS-C121128 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody - LS-C121129 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody - LS-C127086 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal

Anti-KIAA1811 / BRSK1 Antibody - LS-C187781 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody (C-Terminus, APC) - LS-C233203 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody (C-Terminus, AP) - LS-C233202 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody (C-Terminus, Biotin) - LS-C233204 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody (aa339-388) - LS-C200188 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from COLO205 cells using BRSK1 antibody.

Anti-KIAA1811 / BRSK1 Antibody (C-Terminus, PE) - LS-C233206 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody (aa108-139) - LS-C100945 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-KIAA181 antibody in mouse liver tissue lysate (35 ug/lane). KIAA181(arrow) was detected using the purified antibody.

Anti-KIAA1811 / BRSK1 Antibody (C-Terminus, FITC) - LS-C233205 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KIAA1811 / BRSK1 Antibody (aa361-410) - LS-C119165 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from 293 cells, using BRSK1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-KIAA1811 / BRSK1 Antibody (aa355-384, AP) - LS-C233207 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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