BANF1
| Gene Symbol | BANF1 |
|---|---|
| Entrez Gene | 8815 |
| Alt Symbol | BAF, BCRP1, D14S1460, NGPS |
| Species | Human |
| Gene Type | protein-coding |
| Description | barrier to autointegration factor 1 |
| Other Description | barrier-to-autointegration factor|breakpoint cluster region protein 1 |
| Swissprots | O75531 Q6FGG7 O60558 |
| Accessions | EAW74483 EAW74484 EAW74485 EAW74486 O75531 AF044773 AAC08964 AF068235 AAD15901 AF070447 AAC23575 AK311961 BAG34901 BC005942 AAH05942 BC008705 BC107702 AAI07703 BG748852 BU940708 CR542140 CAG46937 NM_001143985 NP_001137457 NM_003860 NP_003851 |
| Function | Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. {ECO:0000269|PubMed:11005805, ECO:0000269|PubMed:12163470, ECO:0000269|PubMed:16680152}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:16495336}. Cytoplasm {ECO:0000269|PubMed:16495336}. Chromosome {ECO:0000269|PubMed:16495336}. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization. |
| Tissue Specificity | Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes. |
BAF CRISPR/Cas9 KO Plasmid (h) - sc-401654 from Santa Cruz Biotechnology
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BAF HDR Plasmid (h) - sc-401654-HDR from Santa Cruz Biotechnology
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BAF Double Nickase Plasmid (h) - sc-401654-NIC from Santa Cruz Biotechnology
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BAF Double Nickase Plasmid (h2) - sc-401654-NIC-2 from Santa Cruz Biotechnology
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BAF CRISPR Activation Plasmid (h) - sc-401654-ACT from Santa Cruz Biotechnology
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BAF CRISPR Activation Plasmid (h2) - sc-401654-ACT-2 from Santa Cruz Biotechnology
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BAF Lentiviral Activation Particles (h) - sc-401654-LAC from Santa Cruz Biotechnology
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BAF Lentiviral Activation Particles (h2) - sc-401654-LAC-2 from Santa Cruz Biotechnology
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BAF siRNA (h) - sc-43627 from Santa Cruz Biotechnology
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BAF shRNA Plasmid (h) - sc-43627-SH from Santa Cruz Biotechnology
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BAF shRNA (h) Lentiviral Particles - sc-43627-V from Santa Cruz Biotechnology
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BANF1 RNAi - H00008815-R01 from Novus Biologicals
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MISSION® esiRNA - EHU121211 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3856 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3856 from Sigma-Aldrich
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BANF1 siRNA (Human) - CRH5813 from Cohesion Biosciences
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BANF1 - MBS8231145 from MyBioSource
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BAF CRISPR/Cas9 KO Plasmid (h2) - sc-401654-KO-2 from Santa Cruz Biotechnology
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BAF HDR Plasmid (h2) - sc-401654-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human BANF1 (NM_003860.3) - SHH244818 from Creative biogene
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BANF1 shRNA Plasmid (Human) - CHH5813 from Cohesion Biosciences
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BANF1 miRNA 3'UTR clone - MiUTR3H-06285 from Creative biogene
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BANF1 miRNA 3'UTR clone - MiUTR3H-06286 from Creative biogene
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