Gene Symbol | THOC3 |
---|---|
Entrez Gene | 84321 |
Alt Symbol | THO3, hTREX45 |
Species | Human |
Gene Type | protein-coding |
Description | THO complex 3 |
Other Description | TEX1 homolog|THO complex subunit 3 |
Swissprots | Q6NZ53 Q96J01 |
Accessions | EAW50534 Q96J01 AW511349 AW628745 BC006849 AAH06849 BC066325 AAH66325 BC068499 AAH68499 BG502664 BU634063 BU781626 HY009859 XM_011534666 XP_011532968 XM_011534667 XP_011532969 XM_011534668 XP_011532970 NM_032361 NP_115737 |
Function | Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. {ECO:0000269|PubMed:11979277, ECO:0000269|PubMed:15833825, ECO:0000269|PubMed:15998806, ECO:0000269|PubMed:17190602, ECO:0000269|PubMed:18974867}. |
Subcellular Location | Nucleus {ECO:0000305}. Nucleus speckle {ECO:0000305}. |
Top Pathways | Spliceosome, RNA transport |
THOC3 CRISPR/Cas9 KO Plasmid (h) - sc-408542 from Santa Cruz Biotechnology
|
||||||||
THOC3 HDR Plasmid (h) - sc-408542-HDR from Santa Cruz Biotechnology
|
||||||||
THOC3 Double Nickase Plasmid (h) - sc-408542-NIC from Santa Cruz Biotechnology
|
||||||||
THOC3 Double Nickase Plasmid (h2) - sc-408542-NIC-2 from Santa Cruz Biotechnology
|
||||||||
THOC3 CRISPR Activation Plasmid (h) - sc-408542-ACT from Santa Cruz Biotechnology
|
||||||||
THOC3 CRISPR Activation Plasmid (h2) - sc-408542-ACT-2 from Santa Cruz Biotechnology
|
||||||||
THOC3 Lentiviral Activation Particles (h) - sc-408542-LAC from Santa Cruz Biotechnology
|
||||||||
THOC3 Lentiviral Activation Particles (h2) - sc-408542-LAC-2 from Santa Cruz Biotechnology
|
||||||||
THOC3 siRNA (h) - sc-63125 from Santa Cruz Biotechnology
|
||||||||
THOC3 shRNA Plasmid (h) - sc-63125-SH from Santa Cruz Biotechnology
|
||||||||
THOC3 shRNA (h) Lentiviral Particles - sc-63125-V from Santa Cruz Biotechnology
|
||||||||
THOC3 RNAi - H00084321-R01 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU105031 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN21316 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND21316 from Sigma-Aldrich
|
||||||||
THOC3 siRNA (Human) - CRJ3443 from Cohesion Biosciences
|
||||||||
THOC3 - MBS8235279 from MyBioSource
|
||||||||
shRNA set against Human THOC3 (NM_032361.2) - SHH426928 from Creative biogene
|
||||||||
Human THOC3 siRNA - orb273944 from Biorbyt
|
||||||||
THOC3 shRNA Plasmid (Human) - CHJ3443 from Cohesion Biosciences
|
||||||||
THOC3 miRNA 3'UTR clone - MiUTR1H-10383 from Creative biogene
|