Gene Symbol | ACTN4 |
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Entrez Gene | 81 |
Alt Symbol | ACTININ-4, FSGS, FSGS1 |
Species | Human |
Gene Type | protein-coding |
Description | actinin, alpha 4 |
Other Description | F-actin cross-linking protein|actinin alpha4 isoform|alpha-actinin-4|focal segmental glomerulosclerosis 1|non-muscle alpha-actinin 4 |
Swissprots | O76048 D6PXK4 A4K467 O43707 |
Accessions | EAW56809 EAW56810 EAW56811 O43707 AA478431 AA847056 AK299955 BAG61782 AK304554 BAG65349 AK309361 AK309363 AK309364 AU118403 BC005033 AAH05033 BC015620 AAH15620 BM841805 BP332596 BX647098 D89980 BAA24447 DQ431186 ABD96103 DQ893439 ABM84365 DQ896777 ABM87776 GU987085 ADG03678 U48734 AAC17470 XM_005259281 XP_005259338 XM_005259282 XP_005259339 XM_006723406 XP_006723469 NM_004924 NP_004915 |
Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). {ECO:0000250|UniProtKB:P57780, ECO:0000269|PubMed:15772161, ECO:0000269|PubMed:22351778, ECO:0000305|PubMed:9508771}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:22351778, ECO:0000269|PubMed:9508771}. Cytoplasm {ECO:0000269|PubMed:22351778, ECO:0000269|PubMed:9508771}. Cell junction {ECO:0000250|UniProtKB:P57780}. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC- 2, and bladder cancer KU-7). {ECO:0000269|PubMed:17289661, ECO:0000269|PubMed:9508771}. |
Tissue Specificity | Widely expressed. {ECO:0000269|PubMed:9508771}. |
Top Pathways | Systemic lupus erythematosus, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Leukocyte transendothelial migration, Amoebiasis |
α-actinin-4 CRISPR/Cas9 KO Plasmid (h) - sc-400548 from Santa Cruz Biotechnology
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α-actinin-4 HDR Plasmid (h) - sc-400548-HDR from Santa Cruz Biotechnology
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α-actinin-4 Double Nickase Plasmid (h) - sc-400548-NIC from Santa Cruz Biotechnology
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α-actinin-4 Double Nickase Plasmid (h2) - sc-400548-NIC-2 from Santa Cruz Biotechnology
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α-actinin-4 CRISPR Activation Plasmid (h) - sc-400548-ACT from Santa Cruz Biotechnology
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α-actinin-4 CRISPR Activation Plasmid (h2) - sc-400548-ACT-2 from Santa Cruz Biotechnology
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α-actinin-4 Lentiviral Activation Particles (h) - sc-400548-LAC from Santa Cruz Biotechnology
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α-actinin-4 Lentiviral Activation Particles (h2) - sc-400548-LAC-2 from Santa Cruz Biotechnology
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α-actinin-4 siRNA (h) - sc-43101 from Santa Cruz Biotechnology
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α-actinin-4 shRNA Plasmid (h) - sc-43101-SH from Santa Cruz Biotechnology
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α-actinin-4 shRNA (h) Lentiviral Particles - sc-43101-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU027731 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2782 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2782 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08264 from Sigma-Aldrich
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ACTN4 siRNA (Human) - CRH0051 from Cohesion Biosciences
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Alpha Actinin 4 RNAi - H00000081-R01 from Novus Biologicals
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ACTN4 - MBS8200315 from MyBioSource
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shRNA set against Human ACTN4 (NM_004924.4) - SHH231066 from Creative biogene
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Human ACTN4 siRNA - orb260553 from Biorbyt
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ACTN4 shRNA Plasmid (Human) - CHH0051 from Cohesion Biosciences
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ACTN4 miRNA 3'UTR clone - MiUTR3H-06971 from Creative biogene
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