Gene Symbol | WRN |
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Entrez Gene | 7486 |
Alt Symbol | RECQ3, RECQL2, RECQL3 |
Species | Human |
Gene Type | protein-coding |
Description | Werner syndrome, RecQ helicase-like |
Other Description | DNA helicase, RecQ-like type 3|Werner syndrome ATP-dependent helicase|exonuclease WRN|recQ protein-like 2 |
Swissprots | A1KYY9 Q14191 |
Accessions | AAC41981 AAF06162 AAR05448 EAW63424 EAW63425 EAW63426 Q14191 AB209652 BAD92889 AF091214 AAC63361 AL833572 AY818673 AAX21098 BP279291 XM_011544639 XP_011542941 XM_011544640 XP_011542942 XR_949470 XR_949471 XR_949472 NM_000553 NP_000544 |
Function | Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation. {ECO:0000250, ECO:0000269|PubMed:11863428, ECO:0000269|PubMed:17563354, ECO:0000269|PubMed:18596042, ECO:0000269| |
Subcellular Location | Nucleus, nucleolus. Nucleus. Nucleus, nucleoplasm. Note=Gamma-irradiation leads to its translocation from nucleoli to nucleoplasm and PML regulates the irradiation- induced WRN relocation. |
WRN CRISPR/Cas9 KO Plasmid (h) - sc-401860 from Santa Cruz Biotechnology
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WRN HDR Plasmid (h) - sc-401860-HDR from Santa Cruz Biotechnology
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WRN Double Nickase Plasmid (h) - sc-401860-NIC from Santa Cruz Biotechnology
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WRN Double Nickase Plasmid (h2) - sc-401860-NIC-2 from Santa Cruz Biotechnology
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WRN CRISPR Activation Plasmid (h) - sc-401860-ACT from Santa Cruz Biotechnology
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WRN CRISPR Activation Plasmid (h2) - sc-401860-ACT-2 from Santa Cruz Biotechnology
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WRN Lentiviral Activation Particles (h) - sc-401860-LAC from Santa Cruz Biotechnology
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WRN Lentiviral Activation Particles (h2) - sc-401860-LAC-2 from Santa Cruz Biotechnology
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WRN siRNA (h) - sc-36843 from Santa Cruz Biotechnology
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WRN shRNA Plasmid (h) - sc-36843-SH from Santa Cruz Biotechnology
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WRN shRNA (h) Lentiviral Particles - sc-36843-V from Santa Cruz Biotechnology
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WRN RNAi - H00007486-R02 from Novus Biologicals
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MISSION® esiRNA - EHU048411 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1746 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1746 from Sigma-Aldrich
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WRN siRNA (Human) - CRH5125 from Cohesion Biosciences
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WRN - MBS8205733 from MyBioSource
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shRNA set against Human WRN (NM_000553.4) - SHH447452 from Creative biogene
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WRN shRNA Plasmid (Human) - CHH5125 from Cohesion Biosciences
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WRN miRNA 3'UTR clone - MiUTR3H-06956 from Creative biogene
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