| Gene Symbol | BRD4 |
|---|---|
| Entrez Gene | 23476 |
| Alt Symbol | CAP, HUNK1, HUNKI, MCAP |
| Species | Human |
| Gene Type | protein-coding |
| Description | bromodomain containing 4 |
| Other Description | bromodomain-containing 4|bromodomain-containing protein 4|chromosome-associated protein |
| Swissprots | Q4G0X8 O60885 O60433 Q96PD3 |
| Accessions | AAC27978 EAW84469 EAW84470 EAW84471 EAW84472 EAW84473 O60885 AF252390 AF386649 AAL26987 AK307664 BC000156 AAH00156 BC008354 AAH08354 BC030158 AAH30158 BC035266 AAH35266 BC038988 AAH38988 BC047888 AAH47888 BC067129 AAH67129 BC091649 AAH91649 DQ580084 KF974357 AHK61044 Y12059 CAA72780 XM_011527854 XP_011526156 XM_011527855 XP_011526157 XM_011527856 XP_011526158 NM_014299 NP_055114 NM_058243 NP_490597 |
| Function | Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical |
| Subcellular Location | Nucleus. Chromosome. Note=Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment. |
| Tissue Specificity | Ubiquitously expressed. {ECO:0000269|PubMed:12543779}. |
BRD4 CRISPR/Cas9 KO Plasmid (h) - sc-400519 from Santa Cruz Biotechnology
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BRD4 HDR Plasmid (h) - sc-400519-HDR from Santa Cruz Biotechnology
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BRD4 Double Nickase Plasmid (h) - sc-400519-NIC from Santa Cruz Biotechnology
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BRD4 Double Nickase Plasmid (h2) - sc-400519-NIC-2 from Santa Cruz Biotechnology
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BRD4 CRISPR Activation Plasmid (h) - sc-400519-ACT from Santa Cruz Biotechnology
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BRD4 CRISPR Activation Plasmid (h2) - sc-400519-ACT-2 from Santa Cruz Biotechnology
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BRD4 Lentiviral Activation Particles (h) - sc-400519-LAC from Santa Cruz Biotechnology
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BRD4 Lentiviral Activation Particles (h2) - sc-400519-LAC-2 from Santa Cruz Biotechnology
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BRD4 siRNA (h) - sc-43639 from Santa Cruz Biotechnology
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Brd4 shRNA Plasmid (h) - sc-43639-SH from Santa Cruz Biotechnology
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Brd4 shRNA (h) Lentiviral Particles - sc-43639-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU076751 from Sigma-Aldrich
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MISSION® siRNA - SIHK0193 from Sigma-Aldrich
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MISSION® siRNA - SIHK0194 from Sigma-Aldrich
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MISSION® siRNA - SIHK0192 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN4050 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND4050 from Sigma-Aldrich
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BRD4 siRNA (Human) - CRH8295 from Cohesion Biosciences
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BRD4 - MBS8226283 from MyBioSource
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shRNA set against Human BRD4 (NM_014299.2) - SHH247618 from Creative biogene
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shRNA set against Human BRD4(NM_058243.2) - SHG104263 from Creative biogene
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Human BRD4 siRNA - orb268797 from Biorbyt
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BRD4 shRNA Plasmid (Human) - CHH8295 from Cohesion Biosciences
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BRD4 miRNA 3'UTR clone - MiUTR1H-00941 from Creative biogene
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BRD4 miRNA 3'UTR clone - MiUTR3H-10196 from Creative biogene
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