Gene Symbol | MTPAP |
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Entrez Gene | 55149 |
Alt Symbol | PAPD1, SPAX4 |
Species | Human |
Gene Type | protein-coding |
Description | mitochondrial poly(A) polymerase |
Other Description | PAP-associated domain-containing protein 1|TUTase 1|poly(A) RNA polymerase, mitochondrial|polynucleotide adenylyltransferase|terminal uridylyltransferase 1 |
Swissprots | D3DRX0 Q6P7E5 Q659E3 Q9NVV4 Q9HA74 |
Accessions | EAW86014 EAW86015 Q9NVV4 AB194709 BAD98252 AK001348 BAA91641 AK022188 BAB13981 AK125211 AY364242 AAQ76801 BC061703 AAH61703 DA086747 NM_018109 NP_060579 |
Function | Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA. {ECO:0000269|PubMed:15547249, ECO:0000269|PubMed:15769737, ECO:0000269|PubMed:18172165, ECO:0000269|PubMed:20970105, ECO:0000269|PubMed:21292163}. |
Subcellular Location | Cytoplasm. Mitochondrion. |
Tissue Specificity | Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle. {ECO:0000269|PubMed:15547249}. |
PAPD1 CRISPR/Cas9 KO Plasmid (h) - sc-412459 from Santa Cruz Biotechnology
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PAPD1 HDR Plasmid (h) - sc-412459-HDR from Santa Cruz Biotechnology
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PAPD1 Double Nickase Plasmid (h) - sc-412459-NIC from Santa Cruz Biotechnology
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PAPD1 Double Nickase Plasmid (h2) - sc-412459-NIC-2 from Santa Cruz Biotechnology
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PAPD1 CRISPR Activation Plasmid (h) - sc-412459-ACT from Santa Cruz Biotechnology
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PAPD1 CRISPR Activation Plasmid (h2) - sc-412459-ACT-2 from Santa Cruz Biotechnology
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PAPD1 Lentiviral Activation Particles (h) - sc-412459-LAC from Santa Cruz Biotechnology
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PAPD1 Lentiviral Activation Particles (h2) - sc-412459-LAC-2 from Santa Cruz Biotechnology
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PAPD1 siRNA (h) - sc-90573 from Santa Cruz Biotechnology
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PAPD1 shRNA Plasmid (h) - sc-90573-SH from Santa Cruz Biotechnology
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PAPD1 shRNA (h) Lentiviral Particles - sc-90573-V from Santa Cruz Biotechnology
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PAPD1 RNAi - H00055149-R01 from Novus Biologicals
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MISSION® esiRNA - EHU014891 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN14728 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND14728 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07028 from Sigma-Aldrich
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MTPAP siRNA (Human) - CRJ0506 from Cohesion Biosciences
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shRNA set against Human MTPAP (NM_018109.3) - SHH346620 from Creative biogene
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Human MTPAP siRNA - orb271007 from Biorbyt
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MTPAP shRNA Plasmid (Human) - CHJ0506 from Cohesion Biosciences
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MTPAP miRNA 3'UTR clone - MiUTR4H-TG05719 from Creative biogene
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