KHDRBS1

Gene Symbol KHDRBS1
Entrez Gene 10657
Alt Symbol Sam68, p62, p68
Species Human
Gene Type protein-coding
Description KH domain containing, RNA binding, signal transduction associated 1
Other Description GAP-associated tyrosine phosphoprotein p62 (Sam68)|KH domain-containing, RNA-binding, signal transduction-associated protein 1|p21 Ras GTPase-activating protein-associated p62|src-associated in mitosis 68 kDa protein
Swissprots D3DPP3 Q8NB97 Q07666 Q99760 Q6PJX7
Accessions EAX07574 EAX07575 EAX07576 EAX07577 Q07666 AK091346 BAC03643 AK293369 BAG56883 AK303218 BAG64305 AK312916 BAG35762 BC000717 AAH00717 BC010132 AAH10132 BC019109 AAH19109 CA442272 CN345570 DB365017 DC351638 DQ895323 ABM86249 M88108 AAA59990 U78971 AAB47504 NM_001271878 NP_001258807 NM_006559 NP_006550 NR_073498 NR_073499
Function Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed:22253824). {ECO:0000269|PubMed:22253824}.
Subcellular Location Nucleus {ECO:0000269|PubMed:1374686}. Membrane {ECO:0000269|PubMed:1374686}.
Tissue Specificity Ubiquitously expressed in all tissue examined. Isoform 1 is expressed at lower levels in brain, skeletal muscle, and liver whereas isoform 3 is intensified in skeletal muscle and in liver. {ECO:0000269|PubMed:9013542}.

SAM68 Antibody - 12538 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using SAM68 Antibody.

Sam 68 (7-1) - sc-1238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Sam 68 (7-1): sc-1238. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil showing nuclear staining.

Sam 68 (H-4) - sc-514468 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Sam 68 (H-4): sc-514468. Western blot analysis of Sam 68 expression in non-transfected 293T: sc-117752 (A), mouse Sam 68 transfected 293T: sc-125954 (B), Jurkat (C) and Raji (D) whole cell lysates.

Sam 68 (C-15) - sc-46627 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Sam 68 (C-17) - sc-46628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Sam 68 (C-17): sc-46628. Western blot analysis of Sam 68 expression in non-transfected: sc-117752 (A) and mouse Sam 68 transfected: sc-125954 (B) 293T whole cell lysates.

Sam 68 (C-20) - sc-333 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Sam 68 (C-20): sc-333. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.

Sam 68 (N-15) - sc-46630 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Sam 68 (N-15): sc-46630. Western blot analysis of Sam 68 expression in HeLa nuclear extract (A) and Raji (B) and Ramos (C) whole cell lysates.

Sam 68 (S-331) - sc-733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Sam 68 (S-331): sc-733. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.

Sam 68 (15) - sc-136062 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Sam 68 (15): sc-136062. Western blot analysis of Sam 68 expression in A-431 whole cell lysate.

Anti-SAM68 antibody - ab56836 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ab56836 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab56836 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SAM68 antibody - ab86239 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SAM68 with ab86239 at 1/1000 (0.2µg/ml). Detection: DAB.

Anti-SAM68 antibody - ab104259 from Abcam

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab104259, at 1/250 dilution, staining SAM68 in formalin-fixed, paraffin-embedded Human colon carcinoma by Immunohistochemistry using DAB staining.

Anti-SAM68 antibody [EPR3231] - ab76471 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3231
All lanes : Anti-SAM68 antibody [EPR3231] (ab76471) at 1/100000 dilutionLane 1 : Hela cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SAM68 antibody [EPR3232] - ab109197 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3232
Overlay histogram showing HeLa cells stained with ab109197 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109197, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-KHDRBS1 / SAM68 Antibody - LS-C145000 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-KHDRBS1 / SAM68 Antibody - LS-C153473 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-KHDRBS1 / SAM68 Antibody (N-Terminus) - LS-C31052 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
NIH 3T3 Cell Lysate

Anti-KHDRBS1 / SAM68 Antibody - LS-C155422 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE SAM68 antibody diluted at 1:1000

Anti-KHDRBS1 / SAM68 Antibody (aa104-153) - LS-C80363 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal heart cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-KHDRBS1 / SAM68 Antibody (aa331-348) IHC-plus™ - LS-B8706 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-KHDRBS1 / SAM68 / p62 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-KHDRBS1 / SAM68 Antibody (aa414-443, APC) - LS-C207145 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KHDRBS1 / SAM68 Antibody (aa414-443, AP) - LS-C207144 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KHDRBS1 / SAM68 Antibody (Tyr145) - LS-C177299 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of Sam 68 (Y145) pAb in extracts from HUVEC cells.

Anti-KHDRBS1 / SAM68 Antibody (aa414-443, Biotin) - LS-C207146 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KHDRBS1 / SAM68 Antibody (aa414-443) - LS-C158224 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Mouse Khdrbs1 Antibody western blot of mouse heart tissue lysates (35 ug/lane). The Khdrbs1 antibody detected the Khdrbs1 protein (arrow).
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