NUAK1
| Gene Symbol | NUAK1 |
|---|---|
| Entrez Gene | 9891 |
| Alt Symbol | ARK5 |
| Species | Human |
| Gene Type | protein-coding |
| Description | NUAK family, SNF1-like kinase, 1 |
| Other Description | AMP-activated protein kinase family member 5|AMPK-related protein kinase 5|NUAK family SNF1-like kinase 1|omphalocele kinase 1 |
| Swissprots | A7MD39 Q96KA8 O60285 |
| Accessions | EAW97768 EAW97769 O60285 AB011109 BAA25463 AK027302 BAB55026 AK289992 BAF82681 BC152462 AAI52463 BC160165 AAI60165 BG831390 BQ694341 CK001516 R61142 NM_014840 NP_055655 |
| Function | Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser- 392' and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppr |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:21317932}. Cytoplasm {ECO:0000269|PubMed:21317932}. |
| Tissue Specificity | Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. Highly up-regulated in colorectal cancer cell lines. {ECO:0000269|PubMed:14982852}. |
ARK5 CRISPR/Cas9 KO Plasmid (h) - sc-403053 from Santa Cruz Biotechnology
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ARK5 HDR Plasmid (h) - sc-403053-HDR from Santa Cruz Biotechnology
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ARK5 Double Nickase Plasmid (h) - sc-403053-NIC from Santa Cruz Biotechnology
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ARK5 Double Nickase Plasmid (h2) - sc-403053-NIC-2 from Santa Cruz Biotechnology
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ARK5 CRISPR Activation Plasmid (h) - sc-403053-ACT from Santa Cruz Biotechnology
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ARK5 CRISPR Activation Plasmid (h2) - sc-403053-ACT-2 from Santa Cruz Biotechnology
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ARK5 Lentiviral Activation Particles (h) - sc-403053-LAC from Santa Cruz Biotechnology
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ARK5 Lentiviral Activation Particles (h2) - sc-403053-LAC-2 from Santa Cruz Biotechnology
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ARK5 siRNA (h) - sc-60203 from Santa Cruz Biotechnology
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ARK5 shRNA Plasmid (h) - sc-60203-SH from Santa Cruz Biotechnology
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ARK5 shRNA (h) Lentiviral Particles - sc-60203-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU100071 from Sigma-Aldrich
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MISSION® siRNA - SIHK1465 from Sigma-Aldrich
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MISSION® siRNA - SIHK1466 from Sigma-Aldrich
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MISSION® siRNA - SIHK1467 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN15574 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15574 from Sigma-Aldrich
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NUAK1 siRNA (Human) - CRH6637 from Cohesion Biosciences
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ARK5/NUAK1 RNAi - H00009891-R02 from Novus Biologicals
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shRNA set against Human NUAK1 (NM_014840.2) - SHH355852 from Creative biogene
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shRNA set against Human NUAK1(NM_014840.2) - SHR009276 from Creative biogene
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NUAK1 shRNA Plasmid (Human) - CHH6637 from Cohesion Biosciences
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NUAK1 miRNA 3'UTR clone - MiUTR1H-06955 from Creative biogene
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