POLA1

Gene Symbol POLA1
Entrez Gene 5422
Alt Symbol NSX, POLA, p180
Species Human
Gene Type protein-coding
Description polymerase (DNA directed), alpha 1, catalytic subunit
Other Description DNA polymerase alpha 1 catalytic subunit|DNA polymerase alpha catalytic subunit|DNA polymerase alpha catalytic subunit p180|DNA polymerase alpha p180 subunit|polymerase (DNA-directed), alpha (70kD)
Swissprots P09884 Q86UQ7
Accessions AAA52318 AAP13534 CAA47340 CAZ65678 CCQ43547 EAW99028 P09884 BM664024 BU508486 BX648513 CN364591 DW462890 X06745 CAA29920 XM_005274552 XP_005274609 XM_006724499 XP_006724562 XM_011545540 XP_011543842 XM_011545541 XP_011543843 NM_016937 NP_058633
Function Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. {ECO:0000269|PubMed:9518481}.
Subcellular Location Nucleus.
Top Pathways DNA replication, Purine metabolism, Pyrimidine metabolism

DNA pol α (D-4) - sc-373884 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA pol α (D-4): sc-373884. Western blot analysis of DNA pol α expression in Jurkat nuclear extract.

DNA pol α (G-16) - sc-5921 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DNA pol α (G-16): sc-5921. Western blot analysis of DNA pol α expression in Jurkat (A,D), K-562 (B) and KNRK (C) nuclear extracts. Antibodies tested include DNA pol α (G-16): sc-5921 (AC) and DNA pol α (N-19): sc-5920 (B).

DNA pol α (F-6) - sc-365040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA pol α (F-6): sc-365040. Western blot analysis of DNA pol α expression in HeLa (A) and Jurkat (B) nuclear extracts.

DNA pol α (G-12) - sc-365039 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA pol α (G-12): sc-365039. Western blot analysis of DNA pol α expression in HeLa (A) and Jurkat (B) nuclear extracts.

DNA pol α (H-230) - sc-48818 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DNA pol α (H-230): sc-48818. Western blot analysis of DNA pol α expression in MOLT-4 (A) and Ramos (B) nuclear extracts.

DNA pol α (G-5) - sc-365327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA pol α (G-5): sc-365327. Western blot analysis of DNA pol α expression in K-562 nuclear extract.

DNA pol α (D-7) - sc-137021 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DNA pol α (D-7): sc-137021. Western blot analysis of DNA pol &alpha expression in K-562 (A), Jurkat (B) and KNRK (C) nuclear extracts.

DNA pol α (N-19) - sc-5920 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DNA pol a (G-16): sc-5921. Western blot analysis of DNA pol a expression in Jurkat (A,D), K-562 (B) and KNRK (C) nuclear extracts. Antibodies tested include DNA pol a (G-16): sc-5921 (AC) and DNA pol a (N-19): sc-5920 (B).

Anti-DNA polymerase alpha antibody - ab65009 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DNA polymerase alpha antibody (ab65009) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from 293 cellsLane 3 : Extracts from HepG2 cellsLane 4 : Extracts from HepG2 cells with immunizing peptideLysates/proteins at 5 µg per lane.

Anti-DNA polymerase alpha antibody - ab57296 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (clone CL22-2-42B) - LS-C179886 from LifeSpan Bioscience

Prices $345.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone CL22-2-42B

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (aa775-825) - LS-C289559 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (aa1363-1463, Azide-free) - LS-C328284 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (aa81-130) - LS-C118771 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HepG2/293/Jurkat cells, using DNA Polymerase alpha Antibody. The lane on the right is treated with the synthesized peptide.

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (Lys111) - LS-C176726 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of DNA pol (K111) pAb in extracts from A549 cells.

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (clone 3C11) - LS-C197940 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C11
POLA monoclonal antibody (M01), clone 3C11 Western Blot analysis of POLA expression in HeLa NE.

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (aa1175-1225) - LS-C289561 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (aa1254-1462) - LS-C311491 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DNA polymerase alpha Antibody - NBP1-82449 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-82449] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.

DNA polymerase alpha Antibody - NBP1-47257 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DNA polymerase alpha Antibody - NBP1-47256 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitope

DNA polymerase alpha Antibody - NB100-92311 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: DNA polymerase alpha Antibody [NB100-92311] - Extracts from HepG2 cells.

DNA polymerase alpha Antibody (3C11) - H00005422-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C11

DNA polymerase alpha Antibody - H00005422-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

DNA polymerase alpha Polyclonal Antibody - bs-6166R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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