POLA1
| Gene Symbol | POLA1 |
|---|---|
| Entrez Gene | 5422 |
| Alt Symbol | NSX, POLA, p180 |
| Species | Human |
| Gene Type | protein-coding |
| Description | polymerase (DNA directed), alpha 1, catalytic subunit |
| Other Description | DNA polymerase alpha 1 catalytic subunit|DNA polymerase alpha catalytic subunit|DNA polymerase alpha catalytic subunit p180|DNA polymerase alpha p180 subunit|polymerase (DNA-directed), alpha (70kD) |
| Swissprots | P09884 Q86UQ7 |
| Accessions | AAA52318 AAP13534 CAA47340 CAZ65678 CCQ43547 EAW99028 P09884 BM664024 BU508486 BX648513 CN364591 DW462890 X06745 CAA29920 XM_005274552 XP_005274609 XM_006724499 XP_006724562 XM_011545540 XP_011543842 XM_011545541 XP_011543843 NM_016937 NP_058633 |
| Function | Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. {ECO:0000269|PubMed:9518481}. |
| Subcellular Location | Nucleus. |
| Top Pathways | DNA replication, Purine metabolism, Pyrimidine metabolism |
DNA pol α CRISPR/Cas9 KO Plasmid (h) - sc-402762 from Santa Cruz Biotechnology
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DNA pol α HDR Plasmid (h) - sc-402762-HDR from Santa Cruz Biotechnology
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DNA pol α Double Nickase Plasmid (h) - sc-402762-NIC from Santa Cruz Biotechnology
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DNA pol α Double Nickase Plasmid (h2) - sc-402762-NIC-2 from Santa Cruz Biotechnology
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DNA pol α CRISPR Activation Plasmid (h) - sc-402762-ACT from Santa Cruz Biotechnology
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DNA pol α CRISPR Activation Plasmid (h2) - sc-402762-ACT-2 from Santa Cruz Biotechnology
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DNA pol α Lentiviral Activation Particles (h) - sc-402762-LAC from Santa Cruz Biotechnology
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DNA pol α Lentiviral Activation Particles (h2) - sc-402762-LAC-2 from Santa Cruz Biotechnology
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DNA pol α siRNA (h) - sc-37771 from Santa Cruz Biotechnology
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DNA pol α shRNA Plasmid (h) - sc-37771-SH from Santa Cruz Biotechnology
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DNA pol α shRNA (h) Lentiviral Particles - sc-37771-V from Santa Cruz Biotechnology
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DNA polymerase alpha RNAi - H00005422-R01 from Novus Biologicals
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MISSION® esiRNA - EHU034181 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2419 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2419 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08064 from Sigma-Aldrich
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POLA1 siRNA (Human) - CRH3625 from Cohesion Biosciences
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Human POLA1 siRNA - orb264127 from Biorbyt
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POLA1 shRNA Plasmid (Human) - CHH3625 from Cohesion Biosciences
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POLA1 miRNA 3'UTR clone - MiUTR3H-03003 from Creative biogene
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