LRWD1

Gene Symbol LRWD1
Entrez Gene 222229
Alt Symbol CENP-33, ORCA
Species Human
Gene Type protein-coding
Description leucine-rich repeats and WD repeat domain containing 1
Other Description ORC-associated protein|centromere protein 33|leucine-rich repeat and WD repeat-containing protein 1|origin recognition complex associated|origin recognition complex-associated protein
Swissprots Q96GJ2 Q9UFC0 A8K4K2 Q8N0T9 B2R9G2 Q8WV43
Accessions EAW50250 Q9UFC0 AB209379 BAD92616 AK290967 BAF83656 AK313771 BAG36509 AL133057 CAB61382 BC009436 AAH09436 BC018769 AAH18769 BC030547 AAH30547 XM_005250204 XP_005250261 NM_152892 NP_690852
Function Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability. {ECO:0000269|PubMed:20850016, ECO:0000269|PubMed:20932478, ECO:0000269|PubMed:21029866, ECO:0000269|PubMed:22427655, ECO:0000269|PubMed:22645314}.
Subcellular Location Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}. Note=Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3.
Tissue Specificity Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS). {ECO:0000269|PubMed:17074343}.

LRWD1 (S-12) - sc-136741 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
LRWD1 (S-12): sc-136741. Western blot analysis of LRWD1 expression in LADMAC (A) and WI 38 (B) whole cell lysates and mouse thymus (C) tissue extract .

LRWD1 (A-12) - sc-136739 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 antibody - ab107779 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-LRWD1 antibody (ab107779) at 1 µg/ml + Testis (Human) Tissue Lysate - adult normal tissue (ab30257) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-LRWD1 antibody - ab85738 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LRWD1 antibody (ab85738) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg

Anti-LRWD1 antibody - C-terminal - ab153868 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LRWD1 antibody - C-terminal (ab153868) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-LRWD1 Antibody (aa1-30, APC) - LS-C262314 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa1-30, AP) - LS-C262313 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa1-30, Azide-free, HRP) - LS-C262317 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa1-30, FITC) - LS-C262316 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa1-30, Biotin) - LS-C262315 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa1-30, PE) - LS-C262318 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa432-482) - LS-C288646 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa4-33) - LS-C322754 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa200-250) - LS-C288645 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-LRWD1 Antibody (aa1-30) - LS-C165901 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
LRWD1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

LRWD1 Antibody - NBP1-85113 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: LRWD1 Antibody [NBP1-85113] - Staining of human cell line U-2 OS shows positivity in nucleus & nucleoli.

LRWD1 Antibody - NBP1-85112 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: LRWD1 Antibody [NBP1-85112] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.

LRWD1 Antibody - NBP1-03331 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LRWD1 Antibody [NBP1-03331] - Detection of Human LRWD1 on HeLa whole cell lysate using NBP1-03331. LRWD1 was successfully immunoprecipitated by rabbit anti-LRWD1 antibody NBP1-03330.

LRWD1 Antibody - NBP2-17176 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LRWD1 Antibody [NBP2-17176] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.

LRWD1 Antibody - NBP1-03330 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LRWD1 Antibody [NBP1-03330] - Detection of Human LRWD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-LRWD1 antibody NBP1-03330 used for IP at 3 mcg/mg lysate

Anti-LRWD1 (C-terminal) antibody produced in rabbit - SAB2701560 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LRWD1 (C-terminal): <B>Cat. No. SAB2701560</B>: Western Blot analysis of LRWD1 in extracts from <B>Lanes</B><BR/>A. HeLa <BR/>B. HepG2 using <B>Cat. No. SAB2701560</B> at a 1:1000 dilution.

Anti-LRWD1 antibody produced in rabbit - HPA029916 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-LRWD1: <B>Cat. No. HPA029916</B>: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Anti-LRWD1 antibody produced in rabbit - HPA021320 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-LRWD1: <B>Cat. No. HPA021320</B>: Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

LRWD1-Antibody-N-term - AP11807a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
LRWD1 Antibody (N-term) (Cat. #AP11807a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LRWD1 antibody detected the LRWD1 protein (arrow).

LRWD1 antibody [C2C3], C-term - GTX122562 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-LRWD1 antibody [C2C3], C-term used in Western blot. GTX122562
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